US4717668A — Plastic roller bottle for suspension cultures

Claim 1

Independent Claim 104 words

A plastic culture bottle which comprises: a closed bottle defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being of a thickness, at least apart from said rings, such that the rate of oxygen and carbon dioxide diffusion through said plastic walls is sufficient to permit the culturing of microorganisms and cells of aerobic metabolism.

Claim 2

Dependent Claim 104 words

A plastic culture bottle which comprises: a closed bottle defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being of a thickness, at least apart from said rings, such that the rate of oxygen and carbon dioxide diffusion through said plastic walls is sufficient to permit the culturing of microorganisms and cells of aerobic metabolism.

Claim 3

Dependent Claim 111 words

A plastic bottle for suspension cultures, which comprises: a closed body defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness, at least apart from said rings, such that an oxygen diffusion of at least 40 cc. and a carbon dioxide diffusion of at least 150 cc. is provided per square meter per hour through said plastic walls.

Claim 4

Dependent Claim 111 words

A plastic bottle for suspension cultures, which comprises: a closed body defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness, at least apart from said rings, such that an oxygen diffusion of at least 40 cc. and a carbon dioxide diffusion of at least 150 cc. is provided per square meter per hour through said plastic walls.

Claim 5

Dependent Claim 23 words

The bottle of claim 2 in which from 400 to 1000 square cm. of said plastic walls apart from said rings are present.

Claim 6

Dependent Claim 41 words

The bottle of claim 2 in which said access means comprises at least one flexible, thermoplastic access tube communicating through said closed body at one end thereof, said access tube having a free end which is sealed to sterile connector means.

Claim 7

Dependent Claim 110 words

The bottle of claim 2 which comprises from 10 to 40 percent by weight of a polyolefin consisting essentially of propylene units; from 40 to 85 percent by weight of a block copolymer, having thermoplastic rubber characteristics, consisting essentially of (1) a central block, comprising 50 to 85 percent by weight of the copolymer molecule, of a rubber olefin polymer of generally equal proportions of ethylene-butylene units, and (2) terminal blocks of polystyrene; and from 10 to 40 percent by weight of a softening agent comprising poly(ethylene-vinyl acetate) containing essentially from 28 to 35 percent by weight of vinyl acetate units, said plastic formulation being essentially free of liquid plasticizers.

Claim 8

Dependent Claim 23 words

The bottle of claim 5 in which the thickness of said plastic walls, apart from said rings, is from 0.012 to 0.04 inch.

Claim 9

Dependent Claim 16 words

The bottle of claim 2 which is proportioned to be usable in a roller-type culturing apparatus.

Claim 10

Dependent Claim 162 words

A plastic bottle for suspension cultures, which comprises: a closed body defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness such that an oxygen diffusion of at least 40cc. and a carbon dioxide diffusion of at least 150cc. is provided per square meter per hour of said plastic walls, at least apart from said rings, there being from 400 to 1000 square centimeters of said plastic walls apart from said rings present, said aseptically sealable access means comprising at least one flexible, thermoplastic access tube communicating through said closed body at one end thereof, said access tube having a free end which is sealed to sterile connector means.

Claim 11

Dependent Claim 162 words

A plastic bottle for suspension cultures, which comprises: a closed body defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness such that an oxygen diffusion of at least 40cc. and a carbon dioxide diffusion of at least 150cc. is provided per square meter per hour of said plastic walls, at least apart from said rings, there being from 400 to 1000 square centimeters of said plastic walls apart from said rings present, said aseptically sealable access means comprising at least one flexible, thermoplastic access tube communicating through said closed body at one end thereof, said access tube having a free end which is sealed to sterile connector means.

Claim 12

Dependent Claim 23 words

The bottle of claim 8 in which the thickness of said plastic walls, apart from said rings, is from 0.012 to 0.04 inch.

Claim 13

Dependent Claim 110 words

The bottle of claim 9 which comprises from 10 to 40 percent by weight of a polyolefin consisting essentially of propylene units; from 40 to 85 percent by weight of a block copolymer, having thermoplastic rubber characteristics, consisting essentially of (1) a central block, comprising 50 to 85 percent by weight of the copolymer molecule, of a rubbery olefin polymer of generally equal proportions of ethylene-butylene units, and (2) terminal blocks of polystyrene; and from 10 to 40 percent by weight of a softening agent comprising poly(ethylene-vinyl acetate) containing essentially from 28 to 35 percent by weight of vinyl acetate units, said plastic formulation being essentially free of liquid plasticizers.

Claim 14

Dependent Claim 16 words

The bottle of claim 10 which is proportioned to be usable in a roller-type culturing apparatus.

Annuity Stage	Window Opens	Payment Deadline	Surcharge Period Until	Estimated Fee	Status
3.5 Years (Year 4)	1991-01-04	1991-07-06	1992-01-05	$2000.00	Window Closed / Lapsed
7.5 Years (Year 8)	1995-01-04	1995-07-06	1996-01-05	$3760.00	Window Closed / Lapsed
11.5 Years (Year 12)	1999-01-04	1999-07-06	2000-01-05	$7700.00	Window Closed / Lapsed

Plastic roller bottle for suspension cultures

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