US3875000A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Cell culture apparatus
Assignee: Merck and Co Inc
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Filing: 1974-04-05
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Granted: 1975-04-01
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Expires: 1994-04-05
Abstract
Abstract A tissue cell culture apparatus wherein a culture bottle is connected to supply and collection manifolds via lengths of flexible tubing and wherein the bottle is rotated in reciprocal fashion so that the flexible tubing does not become pinched or broken.
Inventors
- 👤 Ben Kaneda
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 00:07
CPC Classifications
C; C12; C12M; C12M27/00; C12M27/10; C12M27/12; C12M23/00; C12M23/40; C12M23/48; C12M29/00
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
104 words
TISSUE CULTURE APPARATUS COMPRISING A TISSUE CULTURE BOTTLE, SUPPORT AND DRIVE MEANS SUPPORTING SAID BOTTLE WITH ITS LONGITUDINAL AXIS HORIZONTAL, SAID SUPPORT AND DIRVE MEANS BEING OPERATIVE TO ROTATE SAID BOTTLE ABOUT ITS LINGITUDINAL AXIS RECIPROCALLY FIST IN ONE DIRECTION AND THEN IN THE OPPOSITE DIRECTION, FLUID CONDUIT MEANS FOR EFFECTING TRANSFER OF FLUIDS BETWEEN THE INTERIOR OF THE BOTTLE AND AN EXTERIOR MANIFOLD DURING ROTATION OF SAID BOTTLE SAID FLUID CONDUIT MEANS COMPRISING AT LEAST ONE FLEXIBLE TUBE EXTENDING BETWEEN ONE END OF SAID BOTTLE AND SAID MANIFOLD, SAID FLEXIBLE TUBE BEING FIXEDLY SECURED AT EACH END, RESPECTIVELY, TO SAID BOTTLE AND TO
Claim 2
Dependent Claim
104 words
TISSUE CULTURE APPARATUS COMPRISING A TISSUE CULTURE BOTTLE, SUPPORT AND DRIVE MEANS SUPPORTING SAID BOTTLE WITH ITS LONGITUDINAL AXIS HORIZONTAL, SAID SUPPORT AND DIRVE MEANS BEING OPERATIVE TO ROTATE SAID BOTTLE ABOUT ITS LINGITUDINAL AXIS RECIPROCALLY FIST IN ONE DIRECTION AND THEN IN THE OPPOSITE DIRECTION, FLUID CONDUIT MEANS FOR EFFECTING TRANSFER OF FLUIDS BETWEEN THE INTERIOR OF THE BOTTLE AND AN EXTERIOR MANIFOLD DURING ROTATION OF SAID BOTTLE SAID FLUID CONDUIT MEANS COMPRISING AT LEAST ONE FLEXIBLE TUBE EXTENDING BETWEEN ONE END OF SAID BOTTLE AND SAID MANIFOLD, SAID FLEXIBLE TUBE BEING FIXEDLY SECURED AT EACH END, RESPECTIVELY, TO SAID BOTTLE AND TO
Claim 3
Dependent Claim
30 words
Tissue culture apparatus according to claim 1 wherein said support and drive means is operative to rotate said bottle through a total rotational sector of approximately 360* in each direction.
Claim 4
Dependent Claim
45 words
Tissue culture apparatus according to claim 1 wherein said support and drive means is arranged to rotate said bottle through sectors of approximately 180* in each direction from a neutral position with said flexible tube extending in non-twisted condition between said bottle and said manifold.
Claim 5
Dependent Claim
30 words
Tissue culture apparatus according to claim 1 wherein said support and drive means comprises a pair of elongated rollers extending parallel to each other and supporting the bottle on them.
Claim 6
Dependent Claim
26 words
Tissue culture apparatus according to claim 1 wherein at least two flexible tubes are provided between associated stationary manifolds and corresponding conduits extending into said bottle.
Claim 7
Dependent Claim
72 words
In a method for culturing tissue cells, the steps of supplying nutrients and gases to a bottle containing culture medium and tissue cells, withdrawing fluids from the bottle and passing said fluids through flexible conduits extending between the bottle and stationary manifolds, while supporting said bottle with its longitudinal axis in a horizontal direction and reciprocally rotating said bottle about said axis, first in one direction and then in the opposite direction.
Claim 8
Dependent Claim
72 words
In a method for culturing tissue cells, the steps of supplying nutrients and gases to a bottle containing culture medium and tissue cells, withdrawing fluids from the bottle and passing said fluids through flexible conduits extending between the bottle and stationary manifolds, while supporting said bottle with its longitudinal axis in a horizontal direction and reciprocally rotating said bottle about said axis, first in one direction and then in the opposite direction.
Claim 9
Dependent Claim
34 words
A method according to claim 6 wherein said bottle is rotated by causing at least one roller, on which it is supported, to rotate first in one direction and then in the opposite direction.
Claim 10
Dependent Claim
20 words
a method according to claim 6 wherein said bottle is rotated in each direction over a total of approximately 360*.
Claim 11
Dependent Claim
38 words
A method according to claim 6 wherein said bottle is rotated in each direction a total of approximately 180* from a neutral position where said flexible conduits extend between said bottle and said manifolds in a non-twisted condition.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-03-31 | 1978-09-30 | 1979-04-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-03-31 | 1982-09-30 | 1983-04-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-03-31 | 1986-09-30 | 1987-04-01 | $7700.00 | Window Closed / Lapsed |