US3193460A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and apparatus for culturing micro-organisms
Assignee: Eli Lilly and Co
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Filing: 1961-12-21
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Granted: 1965-07-06
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Expires: 1981-12-21
Abstract
No abstract record available.
Inventors
- 👤 Carl F Krabbc
- 👤 Frederick D Taylor
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 19:32
CPC Classifications
C; C12; C12M; C12M41/00; C12M41/12; C12M41/18; C12M25/00; C12M25/06; C12M27/00; C12M27/10; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822
Claims Analysis
Total Claims: 2
Claim 1
Independent Claim
167 words
THE METHOD OF PRODUCING A CULTURE OF A SURFACEGROWTH MICROORGANISM, WHICH COMPRISES (A) PLACING IN A CYLINDIRCAL CONTAINER A QUANTITY OF NORMALLY SOLID CULTURE MEDIUM WHICH LIQUEFIES UNDER HEAT, (B) STERILIZING THE CONTAINER AND CULTURE MEDIUM AT A TEMPERATURE WHICH LEAVES THE MEDIUM IN LIQUID STATE, (C) ROTATING THE CONTAINER ON ITS AXIS IN A HORIZONTAL POSITION TO FLOW THE LIQUID MEDIUM OVER THE CYLINDRICAL WALL OF THE CONTAINER, (D) MEANWHILE COOLING SAID WALL TO SOLIDIFY THE CULTURE MEDIUM ON SAID WALL TO FORM A SURFACE-GROWTH LAYER ON THE INNER SURFACE THEREOF, (E) INTRODUCING A QUANTITY OF LIQUID INOCULUM AND ROTATING THE CONTAINER TO DISTRIBUTE THE INOCULUM OVER THE SURFACE-GROWTH LAYER TO INOCULATE THE SAME, (F) SUBJECTING THE INOCULATED LAYER TO INCUBATION CONDITIONS TO PRODUCE CULTURE GROWTH THEREON, (G) INTRODUCING A QUANTITY OF HARVEST LIQUID AND ROTATING THE CONTAINER ON ITS AXIS TO WASH THE HARVEST LIQUID OVER THE GROWTH-CONTAINING SURFACE TO FORM A SUSPENSION OF CULTURE ORGANISMS IN THE HARVEST LIQUID, (H) AND RECOVERING SAID SUSPENSION.
Claim 2
Independent Claim
167 words
THE METHOD OF PRODUCING A CULTURE OF A SURFACEGROWTH MICROORGANISM, WHICH COMPRISES (A) PLACING IN A CYLINDIRCAL CONTAINER A QUANTITY OF NORMALLY SOLID CULTURE MEDIUM WHICH LIQUEFIES UNDER HEAT, (B) STERILIZING THE CONTAINER AND CULTURE MEDIUM AT A TEMPERATURE WHICH LEAVES THE MEDIUM IN LIQUID STATE, (C) ROTATING THE CONTAINER ON ITS AXIS IN A HORIZONTAL POSITION TO FLOW THE LIQUID MEDIUM OVER THE CYLINDRICAL WALL OF THE CONTAINER, (D) MEANWHILE COOLING SAID WALL TO SOLIDIFY THE CULTURE MEDIUM ON SAID WALL TO FORM A SURFACE-GROWTH LAYER ON THE INNER SURFACE THEREOF, (E) INTRODUCING A QUANTITY OF LIQUID INOCULUM AND ROTATING THE CONTAINER TO DISTRIBUTE THE INOCULUM OVER THE SURFACE-GROWTH LAYER TO INOCULATE THE SAME, (F) SUBJECTING THE INOCULATED LAYER TO INCUBATION CONDITIONS TO PRODUCE CULTURE GROWTH THEREON, (G) INTRODUCING A QUANTITY OF HARVEST LIQUID AND ROTATING THE CONTAINER ON ITS AXIS TO WASH THE HARVEST LIQUID OVER THE GROWTH-CONTAINING SURFACE TO FORM A SUSPENSION OF CULTURE ORGANISMS IN THE HARVEST LIQUID, (H) AND RECOVERING SAID SUSPENSION.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1968-07-05 | 1969-01-04 | 1969-07-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1972-07-05 | 1973-01-04 | 1973-07-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1976-07-05 | 1977-01-04 | 1977-07-06 | $7700.00 | Window Closed / Lapsed |