US4717668A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Plastic roller bottle for suspension cultures
Assignee: Baxter International Inc
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Filing: 1986-12-12
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Granted: 1988-01-05
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Expires: 2006-12-12
Abstract
Abstract A plastic roller bottle is provided for suspension cultures for bacteriology or other cell growth or maintenance. The roller bottle comprises a closed bottle defining a longitudinal axis and having flexible plastic walls. Aseptically sealable means for access into the closed body are provided as well. A plurality of spaced reinforcement rings are defined in the flexible plastic walls about the longitudinal axis, to cause the body to normally retain a predetermined, generally cylindrical shape. The flexible plastic walls, apart from the rings, are made of a plastic formulation, and are of a thickness such that the oxygen and carbon dioxide diffusion which takes place through said walls is sufficient to support desired biochemical culturing processes taking place within the roller bottle without venting.
Inventors
- 👤 Michael R. Keilman
- 👤 Ronald P. Symbol
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 12:41
CPC Classifications
C; C12; C12M; C12M27/00; C12M27/10; C12M27/12; C12M23/00; C12M23/02; C12M23/08; C12M23/26; C12M37/00; Y; Y10; Y10S; Y10S215/00; Y10S215/03
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
104 words
A plastic culture bottle which comprises: a closed bottle defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being of a thickness, at least apart from said rings, such that the rate of oxygen and carbon dioxide diffusion through said plastic walls is sufficient to permit the culturing of microorganisms and cells of aerobic metabolism.
Claim 2
Dependent Claim
104 words
A plastic culture bottle which comprises: a closed bottle defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being of a thickness, at least apart from said rings, such that the rate of oxygen and carbon dioxide diffusion through said plastic walls is sufficient to permit the culturing of microorganisms and cells of aerobic metabolism.
Claim 3
Dependent Claim
111 words
A plastic bottle for suspension cultures, which comprises: a closed body defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness, at least apart from said rings, such that an oxygen diffusion of at least 40 cc. and a carbon dioxide diffusion of at least 150 cc. is provided per square meter per hour through said plastic walls.
Claim 4
Dependent Claim
111 words
A plastic bottle for suspension cultures, which comprises: a closed body defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness, at least apart from said rings, such that an oxygen diffusion of at least 40 cc. and a carbon dioxide diffusion of at least 150 cc. is provided per square meter per hour through said plastic walls.
Claim 5
Dependent Claim
23 words
The bottle of claim 2 in which from 400 to 1000 square cm. of said plastic walls apart from said rings are present.
Claim 6
Dependent Claim
41 words
The bottle of claim 2 in which said access means comprises at least one flexible, thermoplastic access tube communicating through said closed body at one end thereof, said access tube having a free end which is sealed to sterile connector means.
Claim 7
Dependent Claim
110 words
The bottle of claim 2 which comprises from 10 to 40 percent by weight of a polyolefin consisting essentially of propylene units; from 40 to 85 percent by weight of a block copolymer, having thermoplastic rubber characteristics, consisting essentially of (1) a central block, comprising 50 to 85 percent by weight of the copolymer molecule, of a rubber olefin polymer of generally equal proportions of ethylene-butylene units, and (2) terminal blocks of polystyrene; and from 10 to 40 percent by weight of a softening agent comprising poly(ethylene-vinyl acetate) containing essentially from 28 to 35 percent by weight of vinyl acetate units, said plastic formulation being essentially free of liquid plasticizers.
Claim 8
Dependent Claim
23 words
The bottle of claim 5 in which the thickness of said plastic walls, apart from said rings, is from 0.012 to 0.04 inch.
Claim 9
Dependent Claim
16 words
The bottle of claim 2 which is proportioned to be usable in a roller-type culturing apparatus.
Claim 10
Dependent Claim
162 words
A plastic bottle for suspension cultures, which comprises: a closed body defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness such that an oxygen diffusion of at least 40cc. and a carbon dioxide diffusion of at least 150cc. is provided per square meter per hour of said plastic walls, at least apart from said rings, there being from 400 to 1000 square centimeters of said plastic walls apart from said rings present, said aseptically sealable access means comprising at least one flexible, thermoplastic access tube communicating through said closed body at one end thereof, said access tube having a free end which is sealed to sterile connector means.
Claim 11
Dependent Claim
162 words
A plastic bottle for suspension cultures, which comprises: a closed body defining a longitudinal axis and having flexible plastic walls; aseptically sealable means for access into said closed body; and a plurality of spaced reinforcement rings defined in said flexible plastic walls about said longitudinal axis, to cause said body to normally retain a predetermined, generally cylindrical shape, the flexible plastic walls being made of a plastic formulation and being generally of a thickness such that an oxygen diffusion of at least 40cc. and a carbon dioxide diffusion of at least 150cc. is provided per square meter per hour of said plastic walls, at least apart from said rings, there being from 400 to 1000 square centimeters of said plastic walls apart from said rings present, said aseptically sealable access means comprising at least one flexible, thermoplastic access tube communicating through said closed body at one end thereof, said access tube having a free end which is sealed to sterile connector means.
Claim 12
Dependent Claim
23 words
The bottle of claim 8 in which the thickness of said plastic walls, apart from said rings, is from 0.012 to 0.04 inch.
Claim 13
Dependent Claim
110 words
The bottle of claim 9 which comprises from 10 to 40 percent by weight of a polyolefin consisting essentially of propylene units; from 40 to 85 percent by weight of a block copolymer, having thermoplastic rubber characteristics, consisting essentially of (1) a central block, comprising 50 to 85 percent by weight of the copolymer molecule, of a rubbery olefin polymer of generally equal proportions of ethylene-butylene units, and (2) terminal blocks of polystyrene; and from 10 to 40 percent by weight of a softening agent comprising poly(ethylene-vinyl acetate) containing essentially from 28 to 35 percent by weight of vinyl acetate units, said plastic formulation being essentially free of liquid plasticizers.
Claim 14
Dependent Claim
16 words
The bottle of claim 10 which is proportioned to be usable in a roller-type culturing apparatus.
References Cited (Backward Citations)
US1626425A
Cited
US3223595A
Cited
US3589983A
Cited
US3540700A
Cited
US3904059A
Cited
US3893887A
Cited
US3875000A
Cited
US4036698A
Cited
US4140162A
Cited
US4166768A
Cited
US4178209A
Cited
US4351900A
Cited
US4611643A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1991-01-04 | 1991-07-06 | 1992-01-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1995-01-04 | 1995-07-06 | 1996-01-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1999-01-04 | 1999-07-06 | 2000-01-05 | $7700.00 | Window Closed / Lapsed |