Patent US4710471A successfully refreshed from Google Patents.
US4710471A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Novel vector plasmids
Assignee: KH Neochem Co Ltd
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Filing: 1984-11-05
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Granted: 1987-12-01
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Expires: 2004-11-05
Abstract
Abstract Disclosed are recombinant plasmid vectors constructed of a plasmid autonomously replicable in cells of microorganisms belonging to the genus Corynebacterium or Brevibacterium and a DNA fragment containing a gene expressible by said microorganisms. The recombinant vector plasmids are autonomously replicable in glutamic acid--producing microorganisms and are useful to clone desired DNA fragments in such microorganisms.
Inventors
- 👤 Ryoichi Katsumata
- 👤 Akio Ozaki
- 👤 Tetsuo Oka
- 👤 Akira Furuya
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 09:33
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/74; C12N15/77
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
70 words
A recombinant vector plasmid which is constructed by introducing a DNA fragment containing an Eschcrichia coli, Bacillus or Staphylococcus gene into a plasmid autonomously replicable in and derived from a microorganism belonging to the genus Corynebacterium or Brevibacterium, wherein said recombinant vector plasmid is replicable in said microorganism belonging to the genus Corynebacterium or Brevibacterium and is detectable in said microorganism by expression of the phenotype of said introduced gene.
Claim 2
Dependent Claim
70 words
A recombinant vector plasmid which is constructed by introducing a DNA fragment containing an Eschcrichia coli, Bacillus or Staphylococcus gene into a plasmid autonomously replicable in and derived from a microorganism belonging to the genus Corynebacterium or Brevibacterium, wherein said recombinant vector plasmid is replicable in said microorganism belonging to the genus Corynebacterium or Brevibacterium and is detectable in said microorganism by expression of the phenotype of said introduced gene.
Claim 3
Dependent Claim
15 words
The recombinant vector plasmid according to claim 1, wherein the microorganism is a Corynebacterium glutamicum.
Claim 4
Dependent Claim
27 words
The recombinant vector plasmid according to claim 2, wherein the microorganism is Corynebacterium glutamicum 225-57, ATCC 31808, FERM P-5865; or Corynebacterium glutamicum 225-218, ATCC 31832, FERM P-5954.
Claim 5
Dependent Claim
40 words
Plasmid pCE54, or a plasmid constructed from plasmid pCE54 by deleting a DNA region from said plasmid which does not affect its function to replicate and its resistance to antibiotics or by inserting a foreign DNA fragment into said plasmid.
Claim 6
Dependent Claim
40 words
Plasmid pCE54, or a plasmid constructed from plasmid pCE54 by deleting a DNA region from said plasmid which does not affect its function to replicate and its resistance to antibiotics or by inserting a foreign DNA fragment into said plasmid.
Claim 7
Dependent Claim
41 words
Plasmid pCB101, or a plasmid constructed from the plasmid pCB101 by deleting a DNA region from said plasmid which does not affect its function to replicate and its resistance to antibiotics or by inserting a foreign DNA fragment into said plasmid.
Claim 8
Dependent Claim
41 words
Plasmid pCB101, or a plasmid constructed from the plasmid pCB101 by deleting a DNA region from said plasmid which does not affect its function to replicate and its resistance to antibiotics or by inserting a foreign DNA fragment into said plasmid.
Claim 9
Dependent Claim
41 words
Plasmid pEthrl, or a plasmid constructed from the plasmid pEthrl by deleting a DNA region from said plasmid which does not affect its function to replicate and its resistance to antibiotics or by inserting a foreign DNA fragment into said plasmid.
Claim 10
Dependent Claim
41 words
Plasmid pEthrl, or a plasmid constructed from the plasmid pEthrl by deleting a DNA region from said plasmid which does not affect its function to replicate and its resistance to antibiotics or by inserting a foreign DNA fragment into said plasmid.
Claim 11
Dependent Claim
18 words
A microorganism belonging to the genus Corynebacterium or Brevibacterium harboring the recombinant vector plasmid defined in claim 1.
Claim 12
Dependent Claim
12 words
The microorganism according to claim 7 wherein said microorganism is Corynebacterium glutamicum.
Claim 13
Dependent Claim
16 words
The recombinant vector plasmid according to claim 1, wherein said gene is an Escherichia coli gene.
References Cited (Backward Citations)
GB2076853A
Cited
FR2482622A1
Cited
EP0063763A1
Cited
EP0066129A2
Cited
EP0071023A2
Cited
EP0077548A2
Cited
EP0088166A2
Cited
EP0093611A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-30 | 1991-06-01 | 1991-12-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-30 | 1995-06-01 | 1995-12-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-30 | 1999-06-01 | 1999-12-01 | $7700.00 | Window Closed / Lapsed |