Patent US4705753A successfully refreshed from Google Patents.
US4705753A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Biologically active acrylonitrile-based copolymeric membrane
Assignee: Individual
•
Filing: 1984-06-08
•
Granted: 1987-11-10
•
Expires: 2004-06-08
Abstract
Abstract A membrane is produced by dissolving, in a water-miscible solvent, a water insoluble copolymer of acrylontrile with at least one monomer selected from the group consisting of an aminostyrene, a vinyl pyridine and an N-hydroxy-containing-substituent-acrylamide, casting said solution to form a thin layer of solution, contacting said solution with water thereby to coagulate the copolymer into a film, and washing away from the copolymer film the solution of solvent and water. The amine or hydroxy group of the copolymer is then activated and coupled with a ligand such as glucose isomerase, chymotrypsin or Protein A. The coupled membranes can then be used for biological separations and reactions.
Inventors
- 👤 Harry P. Gregor
- 👤 Paul I. Dalven
- 👤 James R. Hildebrandt
- 👤 Leonard T. Hodgins
- 👤 Anthony J. Laccetti
- 👤 Abraham Shamir
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 15:26
CPC Classifications
B; B01; B01D; B01D67/00; B01D67/0081; B01D67/0093; B01D67/00931; A; A61; A61K; A61K38/00; A61K38/005; B01D71/00; B01D71/06; B01D71/40; B01D71/42; B01D71/421; B01J; B01J20/00; B01J20/28; B01J20/28014; B01J20/28033; C; C07; C07K; C07K1/00; C07K1/14; C07K1/16; C07K1/22; C07K14/00; C07K14/435; C07K14/78; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
141 words
A process for producing a biologically active membrane comprising, in a water-miscible solvent forming a solution of a water insoluble copolymer of acrylonitrile with about 1 to 20.5 mole percent of at least one monomer selected from the group consisting of (a) an aminostyrene, (b) a vinyl pyridine and (c) an N-hydroxy-containing substituted-acrylamide, casting said solution to form a thin layer of solution, contacting said solution with water thereby to coagulate the copolymer into a film, washing away from the copolymer film the solution of solvent and water, activating the copolymer with (a) trichloro-s-triazine or by diazotization if the monomer is aminostyrene (b) cyanogenbromide if the monomer is vinyl pyridine or (c) trichloro-s-triazine if the monomer is an N-hydroxy-containing substituted-acrylamide, and then coupling to the activated copolymer a ligand selected from the group consisting of glucose isomerase, chymotrypsin and Protein A.
Claim 2
Dependent Claim
141 words
A process for producing a biologically active membrane comprising, in a water-miscible solvent forming a solution of a water insoluble copolymer of acrylonitrile with about 1 to 20.5 mole percent of at least one monomer selected from the group consisting of (a) an aminostyrene, (b) a vinyl pyridine and (c) an N-hydroxy-containing substituted-acrylamide, casting said solution to form a thin layer of solution, contacting said solution with water thereby to coagulate the copolymer into a film, washing away from the copolymer film the solution of solvent and water, activating the copolymer with (a) trichloro-s-triazine or by diazotization if the monomer is aminostyrene (b) cyanogenbromide if the monomer is vinyl pyridine or (c) trichloro-s-triazine if the monomer is an N-hydroxy-containing substituted-acrylamide, and then coupling to the activated copolymer a ligand selected from the group consisting of glucose isomerase, chymotrypsin and Protein A.
Claim 3
Dependent Claim
21 words
A process according to claim 1, including the further step of binding an antibody to Protein A coupled to the membrane.
Claim 4
Dependent Claim
12 words
A process according to claim 1, wherein the monomer is an aminostyrene.
Claim 5
Dependent Claim
12 words
A process according to claim 1, wherein the monomer is vinyl pyridine.
Claim 6
Dependent Claim
13 words
A process according to claim 1, wherein the monomer is an N-hydroxy-containing substituted-acrylamide.
Claim 7
Dependent Claim
12 words
A process according to claim 1, wherein the ligand is glucose isomerase.
Claim 8
Dependent Claim
11 words
A process according to claim 1, wherein the ligand is chymotrypsin.
Claim 9
Dependent Claim
12 words
A process according to claim 1, wherein the ligand is Protein A.
Claim 10
Dependent Claim
11 words
A biologically active membrane produced by the process of claim 1.
References Cited (Backward Citations)
US3834990A
Cited
US3808305A
Cited
US3964973A
Cited
US3939041A
Cited
US4033817A
Cited
US4371612A
Cited
US4352884A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-11-09 | 1991-05-11 | 1991-11-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-11-09 | 1995-05-11 | 1995-11-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-11-09 | 1999-05-11 | 1999-11-10 | $7700.00 | Window Closed / Lapsed |