US4695537A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Particles sensitized with detergent-treated antigen for agglutination immunoassay
Assignee: UNIVERSITY F TENNESSEE RESEARCH Corp A NON-PROFIT CORP
•
Filing: 1985-04-25
•
Granted: 1987-09-22
•
Expires: 2005-04-25
Abstract
Abstract An agglutination immunoassay for antibody wherein antigen for sensitizing a solid particulate support is treated with detergent. The resultant antigen-sensitized support is resistant to self-agglutination.
Inventors
- 👤 Preston H. Dorsett
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 15:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/543; G01N33/54313; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808
Claims Analysis
Total Claims: 30
Claim 1
Independent Claim
46 words
A composition, comprising: a solid particulate support and a detergent-treated antigen supported on said support, said antigen being an intracellular parasite antigen, said antigen having been treated with a detergent to provide a solid support sensitized with the detergent-treated antigen which is is resistant to self-agglutination.
Claim 2
Dependent Claim
46 words
A composition, comprising: a solid particulate support and a detergent-treated antigen supported on said support, said antigen being an intracellular parasite antigen, said antigen having been treated with a detergent to provide a solid support sensitized with the detergent-treated antigen which is is resistant to self-agglutination.
Claim 3
Dependent Claim
31 words
In a kit for determining antibody by an agglutination technique, the improvement comprising. said kit including in a reagent container the solid particulate sensitized with the detergent-treated antigen of claim 1.
Claim 4
Dependent Claim
21 words
The kit of claim 2 wherein said kit further includes a test card having a flat surface for receiving assay samples.
Claim 5
Dependent Claim
13 words
The kit of claim 3 wherein the solid particulate support is polystyrene latex.
Claim 6
Dependent Claim
25 words
The kit of claim 3 and further comprising in separate reagent containers a reactive serum control antibody and a non-reactive serum control free of antibody.
Claim 7
Dependent Claim
21 words
The composition of claim 1 wherein said antigen has been treated with a detergent prior to being supported on the support.
Claim 8
Dependent Claim
45 words
In a direct agglutination assay for an antibody employing solid particles sensitized with antigen, the improvement comprising: contacting a sample containing a suspected antibody with, the solid particles sensitized with the detergent-treated antigen of claim 2, said suspected antibody becoming immunobound by said detergent-treated antigen.
Claim 9
Dependent Claim
12 words
The assay of claim 7 wherein the antigen is a viral antigen.
Claim 10
Dependent Claim
13 words
The assay of claim 7 wherein the antigen is a rubella virus antigen.
Claim 11
Dependent Claim
13 words
The composition of claim 6 wherein the solid support is a synthetic polymer.
Claim 12
Dependent Claim
23 words
The composition of claim 10 wherein the synthetic polymer is selected from the group consisting of polyvinylchloride, polystyrene, aminated polystyrene and carboxylated polystyrene.
Claim 13
Dependent Claim
15 words
The composition of claim 10 wherein the antigen is covalently coupled to the solid support.
Claim 14
Dependent Claim
14 words
The composition of claim 10 wherein the antigen is adsorbed on the solid support.
Claim 15
Dependent Claim
13 words
The composition of claim 13 wherein the antigen is a herpes virus antigen.
Claim 16
Dependent Claim
13 words
The composition of claim 13 wherein the detergent is an alkali dodecyl sulfate.
Claim 17
Dependent Claim
12 words
The composition of claim 13 wherein the antigen is a viral antigen.
Claim 18
Dependent Claim
12 words
The composition of claim 16 wherein the antigen is rubella virus antigen.
Claim 19
Dependent Claim
11 words
The composition of claim 16 wherein the antigen is CMV antigen.
Claim 20
Dependent Claim
37 words
A process for producing a solid particulate support sensitized with a detergent-treated antigen, which is resistant to self-agglutination, comprising: contacting an intracellular parasite antigen with a detergent and supporting the detergent-treated antigen on a solid particulate support.
Claim 21
Dependent Claim
37 words
A process for producing a solid particulate support sensitized with a detergent-treated antigen, which is resistant to self-agglutination, comprising: contacting an intracellular parasite antigen with a detergent and supporting the detergent-treated antigen on a solid particulate support.
Claim 22
Dependent Claim
13 words
The process of claim 19 wherein the solid support is a synthetic polymer.
Claim 23
Dependent Claim
23 words
The process of claim 20 wherein the synthetic polymer is selected from the group consisting of polyvinylchloride, polystyrene, aminated polystyrene and carboxylated polystyrene.
Claim 24
Dependent Claim
15 words
The process of claim 20 wherein the antigen is covalently coupled to the solid support.
Claim 25
Dependent Claim
14 words
The process of claim 20 wherein the antigen is adsorbed on the solid support.
Claim 26
Dependent Claim
13 words
The process of claim 23 wherein the antigen is a herpes virus antigen.
Claim 27
Dependent Claim
13 words
The process of claim 23 wherein the detergent is an alkali dodecyl sulfate.
Claim 28
Dependent Claim
12 words
The process of claim 23 wherein the antigen is a viral antigen.
Claim 29
Dependent Claim
12 words
The process of claim 26 wherein the antigen is rubella virus antigen.
Claim 30
Dependent Claim
11 words
The process of claim 26 wherein the antigen is CMV antigen.
References Cited (Backward Citations)
US4060597A
Cited
US4311788A
Cited
US4292038A
Cited
US4362531A
Cited
US4600698A
Cited
US4536478A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-09-21 | 1991-03-23 | 1991-09-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-09-21 | 1995-03-23 | 1995-09-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-09-21 | 1999-03-23 | 1999-09-22 | $7700.00 | Window Closed / Lapsed |