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Patent US4695455A successfully refreshed from Google Patents.
US4695455A
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Cellular encapsulation of pesticides produced by expression of heterologous genes
Assignee: Mycogen Corp
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Filing: 1985-08-30
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Granted: 1987-09-22
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Expires: 2005-08-30
Abstract
Abstract Methods and compositions are provided for preparing and using pesticides, where the pesticides are encapsulated in non-proliferating cells. The methods involve introducing a heterologous gene into a cellular host, where expression of the heterologous gene results, directly or indirectly, in production of the pesticide. These cells are then killed under conditions which prolong the pesticidal activity when said cells are applied to the environment of a target pest. The killed cells can be used directly or after formulation for treatment of an agricultural host or environment of the host with the pesticide.
Inventors
- 👤 Andrew C. Barnes
- 👤 Susan G. Cummings
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 09:51
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/195; A; A01; A01N; A01N63/00; A01N63/50; C07K14/32; C07K14/325; C12; C12N; C12N1/00; C12N1/005; C12N1/20; C12N1/205; C12P; C12P1/00; C12P1/04; C12R; C12R2001/00; C12R2001/01; C12R2001/07
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
103 words
A pesticidal composition comprising pesticide-containing substantially intact microbial cells and an inert carrier wherein: (1) the microbial cells have been stabilized with a chemical reagent selected from the group consisting of halogenating agents, aldehydes, anti-infective, alcohols and histological fixatives under conditions such that the cells have prolonged pesticidal activity when applied to the environment of a target pest; and (2) the pesticide is a polypeptide, which remains intracellular after stabilisation, and is produced as a result of expression of a heterologous gene in the microbial cell, and which pesticide becomes accessible to a target pest upon ingestion of the cell by a pest.
Claim 2
Dependent Claim
103 words
A pesticidal composition comprising pesticide-containing substantially intact microbial cells and an inert carrier wherein: (1) the microbial cells have been stabilized with a chemical reagent selected from the group consisting of halogenating agents, aldehydes, anti-infective, alcohols and histological fixatives under conditions such that the cells have prolonged pesticidal activity when applied to the environment of a target pest; and (2) the pesticide is a polypeptide, which remains intracellular after stabilisation, and is produced as a result of expression of a heterologous gene in the microbial cell, and which pesticide becomes accessible to a target pest upon ingestion of the cell by a pest.
Claim 3
Dependent Claim
15 words
A pesticidal composition, according to claim 1, wherein said cells are prokaryotes or lower eukaryotes.
Claim 4
Dependent Claim
27 words
A pesticidal composition, according to claim 2, wherein said prokaryote cells are selected from the group consisting of Enterobacteriaceae, Bacillaceae, Rhizobiaceae, Spirillaceae, Lactobacillaceae, Pseudomonadaceae, Azotobacteraceae, and Nitrobacteraceae.
Claim 5
Dependent Claim
23 words
A pesticidal composition, according to claim 2, wherein said lower eukaryotic cells are selected from the group consisting of Phycomycetes, Ascomycetes, and Basidiomycetes.
Claim 6
Dependent Claim
17 words
A pesticidal composition, according to claim 1, wherein said cells are a pigmented bacteria, yeasts, or fungi.
Claim 7
Dependent Claim
13 words
A pesticidal composition, according to claim 1, wherein said microbial cells are Pseudomanas.
Claim 8
Dependent Claim
17 words
A pesticidal composition, according to claim 1, wherein said substantially intact microbial cells are stabilized with formalin.
Claim 9
Dependent Claim
17 words
A pesticidal composition, according to claim 1, wherein said substantially intact microbial cells are stabilized with iodine.
Claim 10
Dependent Claim
22 words
A pesticidal composition, according to claim 1, wherein said microbial cells are is Pseudomonas and said toxin is a B. thuringiensis toxin.
Claim 11
Dependent Claim
17 words
A pesticidal composition, according to claim 1, wherein said pesticide polypeptide is a Bacillus thuringiensis pesticide toxin.
Claim 12
Independent Claim
124 words
A method of protecting a host susceptible to an invertebrate pest which comprises applying to the environment of the pest an effective amount of a pesticidal composition which comprises pesticide-containing substantially intact microbial cells wherein: (1) the microbial cells have been stabilized with a chemical reagent selected from the group consisting of halogenating agents, aldehydes, anti-infectives, alcohols and histological fixatives under conditions such that the cells have prolonged pesticidal activity when applied to the environment of a target pest; and (2) the pesticide is a polypeptide, which remains intracellular after stabilisation, and is produced as a result of expression of a heterologous gene in the microbial cell, and which pesticide becomes accessible to the target pest upon ingestion of the cell by a pest.
Claim 13
Independent Claim
124 words
A method of protecting a host susceptible to an invertebrate pest which comprises applying to the environment of the pest an effective amount of a pesticidal composition which comprises pesticide-containing substantially intact microbial cells wherein: (1) the microbial cells have been stabilized with a chemical reagent selected from the group consisting of halogenating agents, aldehydes, anti-infectives, alcohols and histological fixatives under conditions such that the cells have prolonged pesticidal activity when applied to the environment of a target pest; and (2) the pesticide is a polypeptide, which remains intracellular after stabilisation, and is produced as a result of expression of a heterologous gene in the microbial cell, and which pesticide becomes accessible to the target pest upon ingestion of the cell by a pest.
Claim 14
Dependent Claim
15 words
A method, according to claim 11, wherein said microbial cells are prokaryotes or lower eukaryotes.
Claim 15
Dependent Claim
26 words
A method according to claim 12, wherein said prokaryotic cells are selected from the group consisting of Enterobacteriaceae, Bacillaceae, Rhizobiaceae, Spirillaceae, Lactobacillaceae, Pseudomonadaceae, Azotobacteraceae, and Nitrobacteraceae.
Claim 16
Dependent Claim
22 words
A method, according to claim 12, wherein said lower eukaryote microorganisms are selected from the group consisting of Phycomycetes, Ascomycetes, and Basidiomycetes.
Claim 17
Dependent Claim
23 words
A method according to claim 11, wherein said microbial cells are pigmented bacteria, yeasts, or fungi and said pesticide is an insecticidal polypeptide.
Claim 18
Dependent Claim
16 words
A method, according to claim 11, wherein said microbial cells are stabilized by treatment with formalin.
Claim 19
Dependent Claim
16 words
A method, according to claim 11, wherein said microbial cells are stabilized by treatment with iodine.
Claim 20
Dependent Claim
17 words
A method, according to claim 11, wherein said host susceptible to an invertebrate pest is a plant.
Claim 21
Dependent Claim
16 words
A method, according to claim 11, wherein said pesticide polypeptide is a Bacillus thuringiensis pesticide toxin.
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