US4609550A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Bacillus cereus subspecies israelensis
Assignee: University of Western Ontario
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Filing: 1983-01-20
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Granted: 1986-09-02
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Expires: 2003-01-20
Abstract
Abstract Biologically pure mutants of Bacillus cereus subspecies israelensis (also called Bacillus thuringiensis sub. sp. israelensis) which are substantially non-spore-forming but which are capable of forming inclusion bodies toxic to Diptera larvae are described. A spore-free insecticide based on these inclusion bodies as well as the method of producing same are disclosed.
Inventors
- 👤 Philip C. Fitz-James
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 17:29
CPC Classifications
C; C12; C12P; C12P1/00; C12P1/04; A; A01; A01N; A01N63/00; A01N63/20; A01N63/22; C12N; C12N1/00; C12N1/20; C12N1/205; C12R; C12R2001/00; C12R2001/01; C12R2001/07; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832; Y10S435/834
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
43 words
A biologically pure mutant of Bacillus cereus subspecies israelensis which is substantially non-spore-forming and which is selected from the group consisting of mutants bearing deposit numbers 1178, 1179 and 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 2
Dependent Claim
43 words
A biologically pure mutant of Bacillus cereus subspecies israelensis which is substantially non-spore-forming and which is selected from the group consisting of mutants bearing deposit numbers 1178, 1179 and 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 3
Dependent Claim
52 words
A biologically pure Spo III mutant of Bacillus cereus subspecies israelensis which is substantially non-spore-forming while being capable of forming inclusion bodies toxic to insects of the order Diptera in the larval stage, said mutant bearing deposit number 1178 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 4
Dependent Claim
52 words
A biologically pure Spo III mutant of Bacillus cereus subspecies israelensis which is substantially non-spore-forming while being capable of forming inclusion bodies toxic to insects of the order Diptera in the larval stage, said mutant bearing deposit number 1178 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 5
Dependent Claim
52 words
A biologically pure Spo II mutant of Bacillus cereus subspecies israelensis which is substantially non-spore-forming while being capable of forming inclusion bodies toxic to insects of the order Diptera in the larval stage, said mutant bearing deposit number 1179 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 6
Dependent Claim
52 words
A biologically pure Spo II mutant of Bacillus cereus subspecies israelensis which is substantially non-spore-forming while being capable of forming inclusion bodies toxic to insects of the order Diptera in the larval stage, said mutant bearing deposit number 1179 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 7
Dependent Claim
66 words
A biologically pure Spo II mutant of Bacillus cereus subspecies israelensis which is substantially non-spore-forming while being capable of forming inclusion bodies toxic to insects of the order Diptera in the larval stage, said mutant bearing deposit number 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada, and deposit number ATCC 39,152 with the American Type Culture Collection, Rockville, Md., U.S.A.
Claim 8
Dependent Claim
66 words
A biologically pure Spo II mutant of Bacillus cereus subspecies israelensis which is substantially non-spore-forming while being capable of forming inclusion bodies toxic to insects of the order Diptera in the larval stage, said mutant bearing deposit number 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada, and deposit number ATCC 39,152 with the American Type Culture Collection, Rockville, Md., U.S.A.
Claim 9
Independent Claim
88 words
A method for producing a substantially spore-free insecticide active against insects of the order Diptera in the larval stage comprising growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, and separating the toxic inclusion bodies from the culture medium, said mutant being selected from the group of mutants bearing deposit numbers 1178, 1179 and 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 10
Independent Claim
88 words
A method for producing a substantially spore-free insecticide active against insects of the order Diptera in the larval stage comprising growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, and separating the toxic inclusion bodies from the culture medium, said mutant being selected from the group of mutants bearing deposit numbers 1178, 1179 and 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 11
Dependent Claim
40 words
A method as in claim 5 wherein the mutant bears deposit number 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada, and deposit number ATCC 39,152 with the American Type Culture Collection, Rockville, Md., U.S.A.
Claim 12
Independent Claim
100 words
A method for producing a substantially spore-free insecticide active against insects of the order Diptera in the larval stage comprising growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, separating fermentation products from the culture medium and subjecting the separated fermentation products to high shearing stress to liberate the toxic inclusion bodies, said mutant being selected from the group of mutants bearing deposit numbers 1178 and 1179 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 13
Independent Claim
100 words
A method for producing a substantially spore-free insecticide active against insects of the order Diptera in the larval stage comprising growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, separating fermentation products from the culture medium and subjecting the separated fermentation products to high shearing stress to liberate the toxic inclusion bodies, said mutant being selected from the group of mutants bearing deposit numbers 1178 and 1179 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 14
Dependent Claim
26 words
A method as in claim 7 wherein said mutant bears deposit number 1178 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 15
Dependent Claim
26 words
A method as in claim 7 wherein said mutant bears deposit number 1179 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 16
Dependent Claim
21 words
A method as in claim 5 or 6, wherein the toxic inclusion bodies are separated from the culture medium by centrifugation.
Claim 17
Dependent Claim
9 words
A freeze-dried culture of the mutant of claim 2.
Claim 18
Dependent Claim
9 words
A freeze-dried culture of the mutant of claim 3.
Claim 19
Dependent Claim
9 words
A freeze-dried culture of the mutant of claim 4.
Claim 20
Dependent Claim
99 words
An insecticide active against insects of the order Diptera in the larval stage comprising as an active ingredient an effective larva killing concentration of substantially spore-free toxic inclusion bodies prepared by growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, and separating the toxic inclusion bodies from the culture medium, said mutant being selected from the group of mutants bearing deposit numbers 1178, 1179 and 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 21
Dependent Claim
99 words
An insecticide active against insects of the order Diptera in the larval stage comprising as an active ingredient an effective larva killing concentration of substantially spore-free toxic inclusion bodies prepared by growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, and separating the toxic inclusion bodies from the culture medium, said mutant being selected from the group of mutants bearing deposit numbers 1178, 1179 and 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 22
Dependent Claim
111 words
An insecticide active against insects of the order Diptera in the larval stage comprising as an active ingredient an effective larva killing concentration of substantially spore-free toxic inclusion bodies prepared by growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, separating fermentation products from the culture medium and subjecting the separated fermentation products to high shearing stress to liberate the toxic inclusion bodies, said mutant being selected from the group of mutants bearing deposit number 1178 and 1179 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 23
Dependent Claim
111 words
An insecticide active against insects of the order Diptera in the larval stage comprising as an active ingredient an effective larva killing concentration of substantially spore-free toxic inclusion bodies prepared by growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, separating fermentation products from the culture medium and subjecting the separated fermentation products to high shearing stress to liberate the toxic inclusion bodies, said mutant being selected from the group of mutants bearing deposit number 1178 and 1179 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 24
Dependent Claim
12 words
An insecticide as in claim 14 of 15 further comprising a carrier.
Claim 25
Independent Claim
97 words
A method of controlling insects of the order Diptera in the larval stage comprising applying to larval habitats an effective concentration of substantially spore-free toxic inclusion bodies, prepared by growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, and separating the toxic inclusion bodies from the culture medium, said mutant being selected from the group of mutants bearing deposit number 1178, 1179 and 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 26
Independent Claim
97 words
A method of controlling insects of the order Diptera in the larval stage comprising applying to larval habitats an effective concentration of substantially spore-free toxic inclusion bodies, prepared by growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to the stage of formation of toxic inclusion bodies and lysis of the cells, and separating the toxic inclusion bodies from the culture medium, said mutant being selected from the group of mutants bearing deposit number 1178, 1179 and 1180 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 27
Independent Claim
107 words
A method of controlling insects or the order Diptera in the larval stage comprising applying to larval habitats an effective concentration of substantially spore-free toxic inclusion bodies, prepared by growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to stage of formation of toxic inclusion bodies and lysis of the cells, separating fermentation products from the culture medium and subjecting separated fermentation products to high shearing stress to liberate the toxic inclusion bodies, said mutant being selected from the group of mutants bearing deposit numbers 1178 and 1179 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 28
Independent Claim
107 words
A method of controlling insects or the order Diptera in the larval stage comprising applying to larval habitats an effective concentration of substantially spore-free toxic inclusion bodies, prepared by growing a biologically pure non-spore-forming mutant of Bacillus cereus subspecies israelensis in a culture medium to stage of formation of toxic inclusion bodies and lysis of the cells, separating fermentation products from the culture medium and subjecting separated fermentation products to high shearing stress to liberate the toxic inclusion bodies, said mutant being selected from the group of mutants bearing deposit numbers 1178 and 1179 with the culture collection of the University of Western Ontario, London, Ontario, Canada.
Claim 29
Dependent Claim
23 words
A method as in claim 17 or 18 wherein the concentration is at least 10 4 inclusion bodies/ml. in the treated larval habitats.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-09-01 | 1990-03-03 | 1990-09-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-09-01 | 1994-03-03 | 1994-09-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-09-01 | 1998-03-03 | 1998-09-02 | $7700.00 | Window Closed / Lapsed |