US4687664A
Expired / Lapsed
US
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Method of inactivating reproducible pathogens
Assignee: Oesterreichisches Institut fuer Haemoderivate
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Filing: 1984-04-30
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Granted: 1987-08-18
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Expires: 2004-04-30
Abstract
Abstract There is described a method of inactivating reproducible pathogens in preparations containing plasmatic enzymes and proenzymes, activated or non-activated coagulation factors, such as Factors II, V, VII, VIII, IX, X, XIII, "FEIBA", prothrombin complex preparations, plasmatic inhibitors, immunoglobulins or other blood products, such as fibronectin and fibrinogen. In order to break and overcome the protective effect of proteins on pathogens by simultaneously preserving the biological activity and the molecular integrity of the proteins, the preparation is adjusted to a salt concentration of more than 0.5 molar by the addition of ammonium sulfate and is thermally treated, whereupon the ammonium sulfate is removed from the preparation.
Inventors
- 👤 Anton Philapitsch
- 👤 Gunter Wober
- 👤 Johann Eibl
- 👤 Otto Schwarz
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 15:29
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/13; A61K31/14; A61K35/00; A61K35/12; A61K35/14; A61K35/16; A61K38/00; A61K38/16; A61K38/17; A61K38/36; A61K38/37; A61K38/43; A61K38/46; A61K38/48; A61K38/482; A61K38/4846; A61K38/55; A61K39/00; A61K39/395; A61L; A61L2/00; A61L2/02; A61L2/04; A61L2103/00; A61L2103/05; A61L2103/09
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
68 words
A method of inactivating reproducible pathogens in a preparation containing plasmatic enzymes on proenzymes, activated or non-activated coagulation factors, prothrombin complex preparations, plasmatic inhibitors, immunoglobulins or other blood products comprising adding ammonium sulfate to said preparation to attain a salt concentration of more than 0.5 molar, thermally treating said preparation for a period of time sufficient to inactivate the pathogens and removing said ammonium sulfate from said preparation.
Claim 2
Independent Claim
68 words
A method of inactivating reproducible pathogens in a preparation containing plasmatic enzymes on proenzymes, activated or non-activated coagulation factors, prothrombin complex preparations, plasmatic inhibitors, immunoglobulins or other blood products comprising adding ammonium sulfate to said preparation to attain a salt concentration of more than 0.5 molar, thermally treating said preparation for a period of time sufficient to inactivate the pathogens and removing said ammonium sulfate from said preparation.
Claim 3
Dependent Claim
20 words
A method as set forth in claim 1, wherein said preparation has a protein content of from 0.001% to 30%.
Claim 4
Dependent Claim
23 words
A method as set forth in claim 1, wherein said preparation having a 2 to 4 molar ammonium sulfate concentration is thermally treated.
Claim 5
Dependent Claim
31 words
A method as set forth in claim 1, wherein a protein and ammonium sulfate containing precipitate is recovered from said preparation by adding ammonium sulfate, and said precipitate is thermally treated.
Claim 6
Dependent Claim
53 words
A method as set forth in claim 1, wherein said preparation is lyophilized after adding said ammonium sulfate so as to obtain a lyophilisate which is then thermally treated, and an aqueous solvent is added to said thermally treated lyophilisate so as to reconstitute a solution from which said ammonium sulfate is removed.
Claim 7
Dependent Claim
36 words
A method as set forth in claim 1, wherein said thermal treatment is carried out during a period of from 1 second to 100 hours and at a temperature of from 40° C. to 121° C.
Claim 8
Dependent Claim
26 words
A method as set forth in claim 1, wherein the pH of said preparation during treatment is maintained in a range of from 5 to 11.
Claim 9
Dependent Claim
24 words
A method as set forth in claim 7, wherein the pH of said preparation is maintained in a range of from 6.5 to 8.5.
Claim 10
Dependent Claim
22 words
A method as set forth in claim 1, wherein protein stabilizing substances are added to said preparation prior to said thermal treatment.
Claim 11
Dependent Claim
15 words
A method as set forth in claim 9, wherein said protein stabilizing substance is glycine.
Claim 12
Dependent Claim
21 words
A method as set forth in claim 1, wherein antimicrobial substances are added to said preparation prior to said thermal treatment.
Claim 13
Dependent Claim
14 words
A method as set forth in claim 11, wherein said antimicrobial substance is caprylate.
Claim 14
Dependent Claim
24 words
A method as set forth in claim 1, wherein said removal of ammonium sulfate from said preparation is effected by means of semi-permeable membranes.
Claim 15
Dependent Claim
26 words
A method as set forth in claim 1 wherein said activated or non-activated coagulation factors include Factors II, V, VII, VIII, IX, X, XIII and "FEIBA".
Claim 16
Dependent Claim
17 words
A method as set forth in claim 1 wherein said other blood products include fibronectin and fibrinogen.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-08-17 | 1991-02-16 | 1991-08-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-08-17 | 1995-02-16 | 1995-08-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-08-17 | 1999-02-16 | 1999-08-18 | $7700.00 | Window Closed / Lapsed |