US4673573A
Expired / Lapsed
US
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Novel fibrinolytic enzyme compounds
Assignee: BEECHA GROUP
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Filing: 1985-01-09
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Granted: 1987-06-16
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Expires: 2005-01-09
Abstract
Abstract A fibrinolytically active protein conjugate comprising at least one optionally blocked fibrinolytic enzyme linked by way of a site other than the catalytic site responsible for fibrinolytic activity to at least one human protein. Processes from making the conjugates and pharmaceutical compositions containing them are also described.
Inventors
- 👤 Harry Ferres
- 👤 Richard A. G. Smith
- 👤 Andrew J. Garman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 10:56
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; A; A61; A61K; A61K47/00; A61K47/50; A61K47/51; A61K47/68; A61K47/6801; A61K47/6803; A61K47/6811; A61K47/6815; A61P; A61P43/00; A61P7/00; A61P7/02; C12N11/00; C12N11/02; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
67 words
A fibrinolytically active protein conjugate comprising at least one optionally blocked fibrinolytic enzyme linked by way of a site other than the catalytic site responsible for the fibrinolytic activity to at least one intact human protein molecule through a bridging group generated by the reaction of a chemically introduced protein attachment group on one said protein with a chemically introduced linking group on the other said protein.
Claim 2
Dependent Claim
67 words
A fibrinolytically active protein conjugate comprising at least one optionally blocked fibrinolytic enzyme linked by way of a site other than the catalytic site responsible for the fibrinolytic activity to at least one intact human protein molecule through a bridging group generated by the reaction of a chemically introduced protein attachment group on one said protein with a chemically introduced linking group on the other said protein.
Claim 3
Dependent Claim
19 words
A protein conjugate according to claim 1 which comprises one optionally blocked fibrinolytic enzyme linked to one human protein.
Claim 4
Dependent Claim
19 words
A protein conjugate according to claim 1 wherein the human protein is a reversibly or irreversibly blocked fibrinolytic enzyme.
Claim 5
Dependent Claim
58 words
A protein conjugate according to claim 1 wherein the enzyme is linked to the human protein by a bridging group of formula (I) --A--R--B-- (I) in which each of A and B, which may be the same or different, represent ##STR24## or a bond, and R is a linking group containing one or more --(CH 2 )-- units.
Claim 6
Dependent Claim
19 words
A protein conjugate according to claim 1 wherein the fibrinolytic enzyme is selected from tissue-type plasminogen activator or urokinase.
Claim 7
Dependent Claim
74 words
A protein conjugate according to claim 1 which is selected from; Reversibly blocked urokinase linked to human albumin; Reversibly blocked urokinase linked to plasminogen; Urokinase linked to human serum albumin; Urokinase linked to reversibly blocked plasmin; Reversibly blocked urokinase linked to reversibly blocked plasmin; Tissue-type plasminogen activator linked to human serum albumin; Tissue-type plasminogen activator linked to immunoglobulin G; Tissue-type plasminogen activator linked to reversibly blocked plasmin; and Tissue-type plasminogen activator linked to fibrinogen.
Claim 8
Dependent Claim
89 words
A process for preparing a fibrinolytically active protein conjugate according to claim 1 which process comprises reacting together an optionally blocked fibrinolytic enzyme and a human protein, one of which has been modified to include a protein attachment group and the other of which has been modified to include a linking group having a moiety capable of reacting with the protein attachment group, said enzyme being modified at a site other than the the catalytic site responsible for fibrinolytic activity, and, thereafter, if desired, optionally blocking any fibrinolytic enzyme.
Claim 9
Dependent Claim
16 words
A process according to claim 7 wherein the protein attachment group is a reactive thiol group.
Claim 10
Dependent Claim
20 words
A pharmaceutical composition comprising a fibrinolytically active protein conjugate according to claim 1 in combination with a pharmaceutically acceptable carrier.
Claim 11
Dependent Claim
26 words
A method of treating thrombotic disease which comprises administering to the sufferer an effective non-toxic amount of a fibrinolytically active protein conjugate according to claim 1.
Claim 12
Dependent Claim
10 words
A protein conjugate according to claim 1 which is: ##STR25##
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-06-15 | 1990-12-15 | 1991-06-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-06-15 | 1994-12-15 | 1995-06-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-06-15 | 1998-12-15 | 1999-06-16 | $7700.00 | Window Closed / Lapsed |