Patent US4668771A successfully refreshed from Google Patents.
US4668771A
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Method for separating bovine lactoferrin from cow's milk and purifying same
Assignee: Snow Brand Milk Products Co Ltd
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Filing: 1985-12-13
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Granted: 1987-05-26
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Expires: 2005-12-13
Abstract
Abstract This invention discloses a method for separating a highly pure form of bovine lactoferrin from cow's milk and purifying same which comprises preparing an affinity-chromatographic column by fixing a monoclonal antibody against bovine lactoferrin to an insoluble carrier; passing milk or a solution of bovine lactoferrin derived from cow'milk through the affinity-chromatographic column; and then eluting the bovine lactoferrin adsorbed to the affinity-chromatographic column.
Inventors
- 👤 Hiroshi Kawakami
- 👤 Hiroshi Shinmoto
- 👤 Shunichi Dosako
- 👤 Kenkichi Ahiko
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 13:30
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/79; C07K16/00; C07K16/18; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/808; Y10S530/809; Y10S530/827; Y10S530/832
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
63 words
A method for separating a highly pure form of bovine lactoferrin from cow's milk and purifying same which comprises preparing an affinity-chromatographic column by fixing a monoclonal antibody against bovine lactoferrin to an insoluble carrier; passing cow's milk or a solution of bovine lactoferrin derived from cow's milk through the affinity-chromatographic column; and then eluting the bovine lactoferrin adsorbed to the affinity-chromatographic column.
Claim 2
Independent Claim
63 words
A method for separating a highly pure form of bovine lactoferrin from cow's milk and purifying same which comprises preparing an affinity-chromatographic column by fixing a monoclonal antibody against bovine lactoferrin to an insoluble carrier; passing cow's milk or a solution of bovine lactoferrin derived from cow's milk through the affinity-chromatographic column; and then eluting the bovine lactoferrin adsorbed to the affinity-chromatographic column.
Claim 3
Dependent Claim
33 words
A method as claimed in claim 1 wherein the monoclonal antibody against bovine lactoferrin is produced by a hybridoma obtained by fusing spleen lymphocytes from a bovine lactoferrin-immunized mouse with mouse myeloma cells.
Claim 4
Dependent Claim
29 words
A method as claimed in claim 1 wherein the affinity-chromatographic column is prepared by fixing a monoclonal antibody against bovine lactoferrin to a carrier consisting of an affinity gel.
Claim 5
Dependent Claim
39 words
A method as claimed in claim 1 wherein the bovine lactoferrin adsorbed to the affinity-chromatographic column is eluted at a pH of 4.7 or less and the pH of the eluted bovine lactoferrin fraction is imxediately returned to neutrality.
Claim 6
Dependent Claim
19 words
A method as claimed in claim 4 wherein the column is eluted at a pH of 4.3 to 2.7.
References Cited (Backward Citations)
US4229342A
Cited
US4436658A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-05-25 | 1990-11-24 | 1991-05-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-05-25 | 1994-11-24 | 1995-05-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-05-25 | 1998-11-24 | 1999-05-26 | $7700.00 | Window Closed / Lapsed |