US4668584A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for forming 2-D crystals of proteins and macromolecules
Assignee: General Electric Co
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Filing: 1985-12-23
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Granted: 1987-05-26
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Expires: 2005-12-23
Abstract
Abstract A method is provided for forming ordered macromolecular protein arrays by avoiding a binding pathway that leads to densely packed, disordered states during protein crystal growth, by a diffusion limited process or by allowing controlled growth to occur by removal of the initial supported protein layer to a different environment.
Inventors
- 👤 Egidijus E. Uzgiris
- 👤 James R. Yates
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 20:48
CPC Classifications
C; C07; C07K; C07K1/00; C07K1/14; C07K1/30; C07K1/306; Y; Y10; Y10S; Y10S530/00; Y10S530/861; Y10T; Y10T428/00; Y10T428/30; Y10T428/31504; Y10T428/31536; Y10T428/31725; Y10T428/31768
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
71 words
A non-homogeneous method for promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising (1) floating the supported phospholipid film in a buffered saline solution which has been injected with a drop of the macromolecular protein in a nonhomogeneous manner, and (2) allowing the injected macromolecular protein to diffuse toward the supported phospholipid film surface for a period of at least one hour.
Claim 2
Dependent Claim
71 words
A non-homogeneous method for promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising (1) floating the supported phospholipid film in a buffered saline solution which has been injected with a drop of the macromolecular protein in a nonhomogeneous manner, and (2) allowing the injected macromolecular protein to diffuse toward the supported phospholipid film surface for a period of at least one hour.
Claim 3
Dependent Claim
72 words
A non-homogeneous method of promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising (1) floating the supported phospholipid film in a buffered saline solution of the protein at a concentration of 10-200 micrograms/ml until a monolayer of the protein is formed, and (2) removing the resulting supported phospholipid film and floating it in a solution of the same protein at a lower concentration.
Claim 4
Dependent Claim
72 words
A non-homogeneous method of promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising (1) floating the supported phospholipid film in a buffered saline solution of the protein at a concentration of 10-200 micrograms/ml until a monolayer of the protein is formed, and (2) removing the resulting supported phospholipid film and floating it in a solution of the same protein at a lower concentration.
Claim 5
Dependent Claim
74 words
A non-homogeneous method of promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising (1) floating the supported phospholipid film in a buffered saline solution of the protein at a concentration of 100-200 micrograms/ml until a monolayer of the protein is formed, and (2) removing the resulting supported phospholipid film and floating it in a solution of a different protein at a concentration of 10-100 micrograms/ml.
Claim 6
Dependent Claim
74 words
A non-homogeneous method of promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising (1) floating the supported phospholipid film in a buffered saline solution of the protein at a concentration of 100-200 micrograms/ml until a monolayer of the protein is formed, and (2) removing the resulting supported phospholipid film and floating it in a solution of a different protein at a concentration of 10-100 micrograms/ml.
Claim 7
Dependent Claim
48 words
A non-homogeneous method of promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising removing the coated supported phospholipid film and floating it in a salt solution comprising an alkaline metal salt having a concentration of at least 10 mM.
Claim 8
Dependent Claim
48 words
A non-homogeneous method of promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising removing the coated supported phospholipid film and floating it in a salt solution comprising an alkaline metal salt having a concentration of at least 10 mM.
Claim 9
Dependent Claim
64 words
A non-homogeneous method for promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising (1) floating the supported phospholipid film in a buffered saline solution which has been superimposed over a layer of the protein, (2) allowing the macromolecular protein to diffuse toward the supported phospholipid film surface for a period of at least one hour.
Claim 10
Dependent Claim
64 words
A non-homogeneous method for promoting nucleation and crystal growth of at least one macromolecular protein monolayer on a supported phospholipid film comprising (1) floating the supported phospholipid film in a buffered saline solution which has been superimposed over a layer of the protein, (2) allowing the macromolecular protein to diffuse toward the supported phospholipid film surface for a period of at least one hour.
References Cited (Backward Citations)
US4172827A
Cited
US4489133A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-05-25 | 1990-11-24 | 1991-05-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-05-25 | 1994-11-24 | 1995-05-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-05-25 | 1998-11-24 | 1999-05-26 | $7700.00 | Window Closed / Lapsed |