US4172827A
Expired / Lapsed
US
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Method for concentration and purification of antigens and antibodies
Assignee: General Electric Co
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Filing: 1975-08-27
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Granted: 1979-10-30
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Expires: 1995-08-27
Abstract
Abstract In carrying out a purification and/or concentration step of antigens or antibodies, a substrate is immersed in a first aqueous medium containing a specifically reacting antigen to coat said substrate with a monomolecular layer of said specifically reacting antigen. The resulting coated substrate is then immersed in a second aqueous medium containing immunologically reactive antibody specific to the antigen in the first aqueous medium to complex said immunologically reactive antibody with said specifically reacting antigen. The resulting substrate is then immersed in a reagent capable of cleaving the immunological bond between said immunologically reactive antibody and said specifically reactive antigen and forming a solution of said immunologically reactive antibody in the immunological bond-cleaving solution and leaving said specifically reacting antigen coated on said substrate. The method can be reversed for preparing a purified concentration of an immunologically reactive antigen whereby a specifically reacting antibody is substituted for the antigen and the corresponding immunologically reactive antigen is substituted for the antibody.
Inventors
- 👤 Ivar Giaever
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 02:24
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/551; G01N33/553; B; B82; B82Y; B82Y15/00; B82Y30/00; B82Y5/00; G01N33/54366; G01N33/54373; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S530/00; Y10S530/806; Y10S530/82; Y10S530/825; Y10S530/826; Y10T; Y10T428/00; Y10T428/26; Y10T428/263; Y10T428/264; Y10T428/265
Claims Analysis
Total Claims: 43
Claim 1
Independent Claim
113 words
A method of preparing a purified concentration of an immunologically reactive antibody comprising the steps of immersing a belt of substrate material in an aqueous medium containing a corresponding specifically reacting antigen to said antibody to coat said belt with a monomolecular layer of said antigen; immersing a portion of said belt in an aqueous medium containing said antibody to complex said antigen with said antibody; immersing said portion of said belt in a weak acid solution to cleave bonds between said antigen and said antibody, thus forming a solution of said antibody in said weak acid solution and leaving said antigen coated on said belt; and recovering said antibody from said solution.
Claim 2
Independent Claim
113 words
A method of preparing a purified concentration of an immunologically reactive antibody comprising the steps of immersing a belt of substrate material in an aqueous medium containing a corresponding specifically reacting antigen to said antibody to coat said belt with a monomolecular layer of said antigen; immersing a portion of said belt in an aqueous medium containing said antibody to complex said antigen with said antibody; immersing said portion of said belt in a weak acid solution to cleave bonds between said antigen and said antibody, thus forming a solution of said antibody in said weak acid solution and leaving said antigen coated on said belt; and recovering said antibody from said solution.
Claim 3
Dependent Claim
38 words
A method as defined in claim 1 of preparing a purified concentration of an immunologically reactive antigen wherein a specifically reacting antibody is substituted for the antigen and the corresponding immunologically reactive antigen is substituted for the antibody.
Claim 4
Dependent Claim
30 words
A method as defined in claim 1 wherein said antigen is a protein antigen, said antibody is a protein antibody specific to the antigen and said aqueous media are solutions.
Claim 5
Dependent Claim
30 words
A method as defined in claim 2 wherein said antibody is a protein antibody, said antigen is a protein antigen specific to the antibody and said aqueous media are solutions.
Claim 6
Independent Claim
100 words
A method of purifying an immunologically reactive antibody comprising the steps of immersing a substrate in an aqueous medium containing a corresponding specifically reacting antigen to said antibody to coat said substrate with a monomolecular layer of said antigen; immersing said substrate in an aqueous medium containing said antibody to complex said antigen with said antibody; immersing said substrate in a weak acid solution to cleave bonds between said antigen and said antibody, thus forming a solution of said antibody in said weak acid solution and leaving said antigen coated on said substrate; and recoverying said antibody from said solution.
Claim 7
Independent Claim
100 words
A method of purifying an immunologically reactive antibody comprising the steps of immersing a substrate in an aqueous medium containing a corresponding specifically reacting antigen to said antibody to coat said substrate with a monomolecular layer of said antigen; immersing said substrate in an aqueous medium containing said antibody to complex said antigen with said antibody; immersing said substrate in a weak acid solution to cleave bonds between said antigen and said antibody, thus forming a solution of said antibody in said weak acid solution and leaving said antigen coated on said substrate; and recoverying said antibody from said solution.
Claim 8
Dependent Claim
34 words
A method as defined in claim 5 of purifying an immunologically reactive antigen wherein a specifically reacting antibody is substituted for the antigen and the corresponding immunologically reactive antigen is substituted for the antibody.
Claim 9
Dependent Claim
30 words
A method as defined in claim 5 wherein said antigen is a protein antigen, said antibody is a protein antibody specific to the antigen and said aqueous media are solutions.
Claim 10
Dependent Claim
30 words
A method as defined in claim 6 wherein said antibody is a protein antibody, said antigen is a protein antigen specific to the antibody and said aqueous media are solutions.
Claim 11
Independent Claim
115 words
A method of purifying an immunologically reactive antibody comprising the steps of immersing a first substrate in an aqueous medium containing a corresponding specifically reacting antigen to said antibody, immersing said first substrate in an aqueous medium containing said antibody to complex said antigen with said antibody, applying a weak acid solution to a surface of a second substrate to cleave bonds between said antigen and said antibody, abutting a surface of said first substrate having said antigen and antibody thereon to said surface of said second substrate having said weak acid solution thereon, separating said substrates whereby said antibody adheres to said surface of said second substrate in monomolecular layer, and recovering said antibody.
Claim 12
Independent Claim
115 words
A method of purifying an immunologically reactive antibody comprising the steps of immersing a first substrate in an aqueous medium containing a corresponding specifically reacting antigen to said antibody, immersing said first substrate in an aqueous medium containing said antibody to complex said antigen with said antibody, applying a weak acid solution to a surface of a second substrate to cleave bonds between said antigen and said antibody, abutting a surface of said first substrate having said antigen and antibody thereon to said surface of said second substrate having said weak acid solution thereon, separating said substrates whereby said antibody adheres to said surface of said second substrate in monomolecular layer, and recovering said antibody.
Claim 13
Dependent Claim
28 words
A method as defined in claim 9 wherein a specifically reacting antibody is substituted for the antigen and the corresponding immunologically reactive antigen is substituted for the antibody.
Claim 14
Dependent Claim
30 words
A method as defined in claim 9 wherein said antigen is a protein antigen, said antibody is a protein antibody specific to the antigen and said aqueous media are solutions.
Claim 15
Dependent Claim
30 words
A method as defined in claim 10 wherein said antigen is a protein antigen, said antibody is a protein antibody specific to the antigen and said aqueous media are solutions.
Claim 16
Dependent Claim
27 words
A method as defined in claim 1 further including the steps or agitating said aqueous medium containing said immunologically reactive antibody, and agitating said weak acid solution.
Claim 17
Dependent Claim
30 words
A method as defined in claim 13, wherein said antigen is a protein antigen, said antibody is a protein antibody specific to the antigen and said aqueous media are solutions.
Claim 18
Dependent Claim
22 words
A method as defined in claim 13 wherein said agitating of said aqueous medium and said weak acid solution is performed continuously.
Claim 19
Dependent Claim
24 words
A method as defined in claim 13 further including the step of periodically draining and refreshing said aqueous medium containing said immunologically reactive antibody.
Claim 20
Dependent Claim
35 words
A method as defined in claim 16 wherein said purified immunologically reactive antibody is collected by draining said weak acid solution and further including the step of refreshing said weak acid solution after said draining.
Claim 21
Dependent Claim
40 words
A method as defined in claim 9 comprising the additional step of applying a metal coating to said surface of a second substrate prior to applying said weak acid solution thereto to cleave bonds between the antigen and the antibody.
Claim 22
Dependent Claim
14 words
A method as defined in claim 5 wherein said substrate is a metallized slide.
Claim 23
Dependent Claim
22 words
A method as defined in claim 19 wherein the metal comprising the substrate is selected from indium, gold, silver, tin and lead.
Claim 24
Dependent Claim
16 words
A method as defined in claim 20 wherein the substrate comprises indium on a glass slide.
Claim 25
Dependent Claim
16 words
A method as defined in claim 20 wherein the substrate comprises gold on a glass slide.
Claim 26
Dependent Claim
26 words
A method as defined in claim 20 wherein the substrate comprises a glass slide coated with a thin indium layer and overcoated with a gold layer.
Claim 27
Dependent Claim
27 words
A method as defined in claim 20 wherein the metallized slide also includes a metal oxide layer intermediate the metal of the slide and said monomolecular layer.
Claim 28
Dependent Claim
20 words
A method as defined in claim 24 wherein said metal is titanium and said oxide is an oxide of titanium.
Claim 29
Dependent Claim
30 words
A method as defined in claim 19 wherein said antigen is a protein antigen, said antibody is a protein antibody specific to the antigen and said aqueous media are solutions.
Claim 30
Dependent Claim
21 words
A method as defined in claim 1 wherein said substrate comprises a plurality of metallized slides affixed to a flexible belt.
Claim 31
Dependent Claim
22 words
A method as defined in claim 27 wherein the metal comprising the substrate is selected from indium, gold, silver, tin and lead.
Claim 32
Dependent Claim
16 words
A method as defined in claim 28 wherein the substrate comprises indium on a glass slide.
Claim 33
Dependent Claim
16 words
A method as defined in claim 28 wherein the substrate comprises gold on a glass slide.
Claim 34
Dependent Claim
26 words
A method as defined in claim 28 wherein the substrate comprises a glass slide coated with a thin indium layer and overcoated with a gold layer.
Claim 35
Dependent Claim
27 words
A method as defined in claim 28 wherein the metallized slide also includes a metal oxide layer intermediate the metal of the slide and said monomolecular layer.
Claim 36
Dependent Claim
20 words
A method as defined in claim 32 wherein said metal is titanium and said oxide is an oxide of titanium.
Claim 37
Dependent Claim
30 words
A method as defined in claim 27 wherein said antigen is a protein antigen, said antibody is a protein antibody specific to the antigen and said aqueous media are solutions.
Claim 38
Independent Claim
132 words
A method of preparing a purified concentration of an immunologically reactive antibody comprising the steps of immersing a belt of substrate material in an aqueous medium containing a corresponding specifically reacting antigen to said antibody to coat said belt with a monomolecular layer of said antigen; immersing a portion of said belt in an aqueous medium containing said antibody to complex said antigen with said antibody; immersing said portion of said belt in a solution of an immunological bond-cleaving reagent to cleave bonds between said antigen and said antibody, said solution being selected from the group of a base solution and a strong salt solution, thus forming a solution of said antibody in said bond-cleaving reagent solution and leaving said antigen coated on said belt; and recovering said antibody from said solution.
Claim 39
Independent Claim
132 words
A method of preparing a purified concentration of an immunologically reactive antibody comprising the steps of immersing a belt of substrate material in an aqueous medium containing a corresponding specifically reacting antigen to said antibody to coat said belt with a monomolecular layer of said antigen; immersing a portion of said belt in an aqueous medium containing said antibody to complex said antigen with said antibody; immersing said portion of said belt in a solution of an immunological bond-cleaving reagent to cleave bonds between said antigen and said antibody, said solution being selected from the group of a base solution and a strong salt solution, thus forming a solution of said antibody in said bond-cleaving reagent solution and leaving said antigen coated on said belt; and recovering said antibody from said solution.
Claim 40
Independent Claim
119 words
A method of purifying an immunologically reactive antibody comprising the steps of immersing a substrate in an aqueous medium containing a corresponding specifically reacting anitgen to said antibody to coat said substrate with a mono-molecular layer of said antigen; immersing said substrate in an aqueous medium containing said antibody to complex said antigen with said antibody; immersing said substrate in a solution of an immunological bond-cleaving reagent to cleave bonds between said antigen and said antibody said solution being selected from the group of a base solution and a strong salt solution, thus forming a solution of said antibody in said bond-cleaving reagent solution and leaving said antigen coated on said substrate; and recovering said antibody from said solution.
Claim 41
Independent Claim
119 words
A method of purifying an immunologically reactive antibody comprising the steps of immersing a substrate in an aqueous medium containing a corresponding specifically reacting anitgen to said antibody to coat said substrate with a mono-molecular layer of said antigen; immersing said substrate in an aqueous medium containing said antibody to complex said antigen with said antibody; immersing said substrate in a solution of an immunological bond-cleaving reagent to cleave bonds between said antigen and said antibody said solution being selected from the group of a base solution and a strong salt solution, thus forming a solution of said antibody in said bond-cleaving reagent solution and leaving said antigen coated on said substrate; and recovering said antibody from said solution.
Claim 42
Independent Claim
139 words
A method of purifying an immunologically reactive antibody comprising the steps of immersing a first substrate in an aqueous medium containing a corresponding specifically reacting antigen to said antibody, immersing said first substrate in an aqueous medium containing said antibody to complex said antigen with said antibody, applying a bond-cleaving reagent solution selected from the group of a base solution and a strong salt solution to a surface of a second substrate, to cleave bonds between said antibody and said antigen, abutting a surface of said first substrate having said antigen and antibody thereon to said first substrate having said antigen and antibody thereon to said surface of said second substrate having said bond-cleaving reagent solution thereon, separating said substrate whereby said antibody adheres to said surface of said second substrate in a monomolecular layer, and recovering said antibody.
Claim 43
Independent Claim
139 words
A method of purifying an immunologically reactive antibody comprising the steps of immersing a first substrate in an aqueous medium containing a corresponding specifically reacting antigen to said antibody, immersing said first substrate in an aqueous medium containing said antibody to complex said antigen with said antibody, applying a bond-cleaving reagent solution selected from the group of a base solution and a strong salt solution to a surface of a second substrate, to cleave bonds between said antibody and said antigen, abutting a surface of said first substrate having said antigen and antibody thereon to said first substrate having said antigen and antibody thereon to said surface of said second substrate having said bond-cleaving reagent solution thereon, separating said substrate whereby said antibody adheres to said surface of said second substrate in a monomolecular layer, and recovering said antibody.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1982-10-29 | 1983-04-30 | 1983-10-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1986-10-29 | 1987-04-30 | 1987-10-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1990-10-29 | 1991-04-30 | 1991-10-30 | $7700.00 | Window Closed / Lapsed |