US4489133A
Expired / Lapsed
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Two-dimensional crystallization technique
Assignee: Leland Stanford Junior University
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Filing: 1982-11-16
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Granted: 1984-12-18
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Expires: 2002-11-16
Abstract
Abstract Methods and compositions involving two-dimensional ordering of organic polar solvent soluble organic macromolecules. Lipid monolayer forming molecules are bound covalently or non-covalently to organic macromolecules soluble in polar solvents, whereby the macromolecules are allowed to become oriented in two-dimensions to form an ordered, low free energy, including crystalline, layer of macromolecules. The macromolecules may then be further combined with other moieties to create additional layers, to be cross-linked or otherwise modified, and/or separated from the lipid monolayer. The resulting ordered layers can be used for a variety of purposes, in crystallographic techniques for structure determination and structural relationship determinations, in optics, as polymers, films, and the like.
Inventors
- 👤 Roger D. Kornberg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 07:59
CPC Classifications
C; C30; C30B; C30B7/00; C30B7/14; A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1274; B; B05; B05D; B05D1/00; B05D1/18; B05D1/185; B82; B82Y; B82Y30/00; B82Y40/00; C30B29/00; C30B29/54; B05D1/20; B05D1/202; Y; Y10; Y10T; Y10T428/00; Y10T428/30; Y10T428/31504; Y10T428/31536; Y10T428/31725; Y10T428/31768
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
117 words
A method for ordering molecules in a lower free energy state as a monomolecular layer, wherein said molecules are characterized by being at least partially soluble in a polar medium, capable of forming an ordered array, capable of binding to a surfactant having lipophilic and hydrophilic regions, wherein said surfactant includes means for binding to said molecule in said hydrophilic region; said method comprising: combining under binding conditions a solution of said molecules in a polar solvent with a monolayer of said surfactant situated at about the surface of said solution, whereby said molecules bind to said surfactant; and incubating the mixture for a time sufficient to allow for ordering of said molecules as a monomolecular layer.
Claim 2
Independent Claim
117 words
A method for ordering molecules in a lower free energy state as a monomolecular layer, wherein said molecules are characterized by being at least partially soluble in a polar medium, capable of forming an ordered array, capable of binding to a surfactant having lipophilic and hydrophilic regions, wherein said surfactant includes means for binding to said molecule in said hydrophilic region; said method comprising: combining under binding conditions a solution of said molecules in a polar solvent with a monolayer of said surfactant situated at about the surface of said solution, whereby said molecules bind to said surfactant; and incubating the mixture for a time sufficient to allow for ordering of said molecules as a monomolecular layer.
Claim 3
Dependent Claim
17 words
A method according to claim 1, wherein said monolayer of surfactant is bound to a solid surface.
Claim 4
Independent Claim
22 words
A method according to any of claims 1 or 2, wherein said molecules are macromolecules of at least about 5,000 molecular weight.
Claim 5
Dependent Claim
12 words
A method according to claim 3, wherein said molecules are poly(amino acids).
Claim 6
Dependent Claim
17 words
A method according to claim 3, wherein said molecules are polynucleotides and said means is an oligonucleotide.
Claim 7
Dependent Claim
52 words
A method according to claim 3, including the additional step of combining said monolayer of molecules with a solution of second molecules in a polar solvent, and incubating the mixture for a sufficient time for said second molecules to bind to said molecules as an ordered second monomolecular layer of second molecules.
Claim 8
Independent Claim
101 words
A method for orienting organic receptors in a lower free energy state as a monomolecular layer, wherein said receptors are members of a specific binding pair consisting of ligand and receptor, by employing surfactants having lipophilic and hydrophilic regions and ligand bound to said surfactant in said hydrophilic region; combining a solution of said receptors in a polar medium under ligand-receptor binding conditions with a monolayer of said surfactant situated at about the surface of said solution; incubating the mixture for a sufficient time for said receptors to bind to said ligand and assume an ordered array as a monomolecular layer.
Claim 9
Independent Claim
101 words
A method for orienting organic receptors in a lower free energy state as a monomolecular layer, wherein said receptors are members of a specific binding pair consisting of ligand and receptor, by employing surfactants having lipophilic and hydrophilic regions and ligand bound to said surfactant in said hydrophilic region; combining a solution of said receptors in a polar medium under ligand-receptor binding conditions with a monolayer of said surfactant situated at about the surface of said solution; incubating the mixture for a sufficient time for said receptors to bind to said ligand and assume an ordered array as a monomolecular layer.
Claim 10
Dependent Claim
11 words
A method according to claim 7, wherein said receptors are antibodies.
Claim 11
Dependent Claim
7 words
A composition prepared according to claim 1.
Claim 12
Dependent Claim
7 words
A composition prepared according to claim 7.
References Cited (Backward Citations)
US4172827A
Cited
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