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US4667018A
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Process for the purification of proteins using acidic polysaccharide gels
Assignee: OLEOFINA SA
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Filing: 1986-02-06
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Granted: 1987-05-19
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Expires: 2006-02-06
Abstract
Abstract A process for the purification of proteins from a fluid medium wherein said proteins have an isoelectric pH higher than the pH of said fluid medium, said process comprising passing the liquid medium over acidic polysaccharide gel particles of at least 0.5 mm in the shortest dimension, and recovering the desired proteins from the gel particles by elution with an aqueous salt solution having ions concentration of at least 5 grams per liter.
Inventors
- 👤 Jean-Paul Prieels
- 👤 Robert Peiffer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 20:29
CPC Classifications
A; A23; A23J; A23J1/00; A23J1/20; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/832; Y10S530/833
Claims Analysis
Total Claims: 31
Claim 1
Independent Claim
96 words
A process for the recovery of milk proteins have an from a fluid medium wherein said proteins have an isoelectric pH higher than the pH of said fluid medium, said process comprising the steps of: (a) passing the liquid medium over gel particles of an acidic polysaccharide under cationic conditions to keep the gel particles insoluble, said particles measuring not less than 0.5 mm in their shortest dimension and wherein the content of acidic polysaccharide in the gel is from about 1 to about 8% by weight; and (b) eluting the proteins from the gel particles.
Claim 2
Dependent Claim
96 words
A process for the recovery of milk proteins have an from a fluid medium wherein said proteins have an isoelectric pH higher than the pH of said fluid medium, said process comprising the steps of: (a) passing the liquid medium over gel particles of an acidic polysaccharide under cationic conditions to keep the gel particles insoluble, said particles measuring not less than 0.5 mm in their shortest dimension and wherein the content of acidic polysaccharide in the gel is from about 1 to about 8% by weight; and (b) eluting the proteins from the gel particles.
Claim 3
Dependent Claim
35 words
The process of claim 1, wherein the fluid medium is selected from the group consisting of milk and milk derivatives and wherein the recovered proteins are proteins having an isoelectric pH of at least 7.5.
Claim 4
Dependent Claim
19 words
The process of claim 1, wherein the acidic polysaccharide is selected from the group consisting of alginates and carrageenins.
Claim 5
Dependent Claim
41 words
The process of claim 1, wherein the gel particles are formed by contacting an aqueous solution of the polysaccharide salt with a cation selected from the group consisting of a suitable alkali metal, alkaline-earth metal, iron, ammonium and any combination thereof.
Claim 6
Dependent Claim
25 words
The process of claim 1, wherein the gel particles have a specific surface area of from about 2×10 -3 to about 0.2 m 2 /g.
Claim 7
Dependent Claim
26 words
The process of claim 1, wherein the amount of gel particles utilized is from about 1% to about 20% by weight based on the fluid medium.
Claim 8
Dependent Claim
29 words
The process of claim 1, wherein the gel particles contain not more than 3% by weight of a metal oxide wherein the metal oxide is insoluble under process conditions.
Claim 9
Dependent Claim
27 words
The process of claim 7, wherein the metal oxide is selected from the group consisting of titanium, zirconium, silicon and aluminum oxides, sepiolite and any combination thereof.
Claim 10
Dependent Claim
13 words
The process of claim 2, wherein the proteins comprise lactoferrin, lactoperoxydase and immunoglobulins.
Claim 11
Dependent Claim
75 words
A process for the purification of proteins from a fluid medium wherein said proteins have an isoelectric pH higher than the pH of said fluid medium, said process comprising: (a) passing the liquid medium over acidic polysaccharide gel particles of at least 0.5 mm in the shortest dimension; and (b) recovering the desired proteins from the gel particles by elution with an aqueous salt solution having ions concentration of at least 5 grams per liter.
Claim 12
Dependent Claim
75 words
A process for the purification of proteins from a fluid medium wherein said proteins have an isoelectric pH higher than the pH of said fluid medium, said process comprising: (a) passing the liquid medium over acidic polysaccharide gel particles of at least 0.5 mm in the shortest dimension; and (b) recovering the desired proteins from the gel particles by elution with an aqueous salt solution having ions concentration of at least 5 grams per liter.
Claim 13
Dependent Claim
35 words
The process of claim 10, wherein the fluid medium is selected from the group consisting of milk and milk derivatives and wherein the recovered proteins are proteins having an isoelectric pH of at least 7.5.
Claim 14
Dependent Claim
21 words
The process of claim 10, wherein the gel particiles contain from about 1 to about 8 wt. % of acidic polysaccharide.
Claim 15
Dependent Claim
19 words
The process of claim 12, wherein the acidic polysaccharide is selected from the group consisting of alginates and carrageenins.
Claim 16
Dependent Claim
41 words
The process of claim 10, wherein the gel particles are formed by contacting an aqueous solution of the polysaccharide salt with a cation selected from the group consisting of a suitable alkali metal, alkaline-earth metal, iron, ammonium and any combination thereof.
Claim 17
Dependent Claim
25 words
The process of claim 10, wherein the gel particles have a specific surface area of from about 2×10 -3 to about 0.2 m 2 /g.
Claim 18
Dependent Claim
26 words
The process of claim 12, wherein the amount of gel particles utilized is from about 1% to about 20% by weight based on the fluid medium.
Claim 19
Dependent Claim
29 words
The process of claim 10, wherein the gel particles contain not more than 3% by weight of a metal oxide wherein the metal oxide is insoluble under process conditions.
Claim 20
Dependent Claim
27 words
The process of claim 17, wherein the metal oxide is selected from the group consisting of titanium, zirconium, silicon and aluminum oxides, sepiolite and any combination thereof.
Claim 21
Dependent Claim
13 words
The process of claim 11, wherein the proteins comprise lactoferrin, lactoperoxydase and immunoglobulins.
Claim 22
Dependent Claim
71 words
A process for recovering proteins having an isoelectric pH of at least 7.5 from milk and milk derivatives, comprising the steps of: (a) passing the milk over gel particles of at least 0.5 mm in the shortest dimension and containing acidic polysaccharide to retain proteins found in the milk; and (b) eluting the proteins from the gel particles with a salt solution having a salt concentration of at least 5 g/l.
Claim 23
Dependent Claim
71 words
A process for recovering proteins having an isoelectric pH of at least 7.5 from milk and milk derivatives, comprising the steps of: (a) passing the milk over gel particles of at least 0.5 mm in the shortest dimension and containing acidic polysaccharide to retain proteins found in the milk; and (b) eluting the proteins from the gel particles with a salt solution having a salt concentration of at least 5 g/l.
Claim 24
Dependent Claim
19 words
The process of claim 20, wherein the acidic polysaccharide is selected from the group consisting of alginates and carrageenins.
Claim 25
Dependent Claim
39 words
The process of claim 21, wherein the gel particles are formed by contacting an aqueous solution of the acidic polysaccharide with a cation selected from the group consisting of alkali metal, alkaline-earth metal, iron, ammonium and any combination thereof.
Claim 26
Dependent Claim
25 words
The process of claim 20, wherein the gel particles have a specific surface area of from about 2×10 -3 to about 0.2 m 2 /g.
Claim 27
Dependent Claim
26 words
The process of claim 20, wherein the amount of gel particles utilized is from about 1% to about 20% by weight based on the fluid medium.
Claim 28
Dependent Claim
29 words
The process of claim 20, wherein the gel particles contain not more than 3% by weight of a metal oxide wherein the metal oxide is insoluble under process conditions.
Claim 29
Dependent Claim
27 words
The process of claim 25, wherein the metal oxide is selected from the group consisting of titanium, zirconium, silicon and aluminum oxides, sepiolite and any combination thereof.
Claim 30
Dependent Claim
13 words
The process of claim 20, wherein the proteins comprise lactoferrin, lactoperoxydase and immunoglobulins.
Claim 31
Dependent Claim
13 words
The process of claim 20 carried out in batch or under continuous conditions.
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