Patent US4663161A successfully refreshed from Google Patents.
US4663161A
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US
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Liposome methods and compositions
Assignee: BERSTEIN IRVING
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Filing: 1985-04-22
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Granted: 1987-05-05
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Expires: 2005-04-22
Abstract
Abstract The present disclosure relates to novel liposome compositions and methods for their preparation. Utilization of the present invention provides an efficient reconstitution of membrane proteins into large (0.1 to 2 micron diameter) phospholipid vesicles with a large, internal aqueous space. The method has been exemplified with the use of glycoproteins of influenza (A/PR8/34) and Sendai (parainfluenza type I) viruses.
Inventors
- 👤 Raphael J. Mannino
- 👤 Susan G. Fogerite
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 21:43
CPC Classifications
A; A61; A61K; A61K9/00; A61K9/10; A61K9/127; A61K9/1271; A61K39/00; C; C12; C12N; C12N2810/00; C12N2810/50; C12N2810/60; C12N2810/6072; Y; Y10; Y10S; Y10S424/00; Y10S424/812; Y10S436/00; Y10S436/829; Y10T; Y10T428/00; Y10T428/29; Y10T428/2982; Y10T428/2984
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
67 words
A membrane protein containing phospholipid vesicle composition characterized in having (A) a large internal aqueous space; (B) a size distribution in the range of from about 0.1 to about 2 microns; (C) the bilayer of said vesicles containing membrane proteins substantially integrated in the lipid bilayer morphologically indistinguishable from those formed on the native organism; and (D) the biological activity of said membrane protein is essentially preserved.
Claim 2
Dependent Claim
67 words
A membrane protein containing phospholipid vesicle composition characterized in having (A) a large internal aqueous space; (B) a size distribution in the range of from about 0.1 to about 2 microns; (C) the bilayer of said vesicles containing membrane proteins substantially integrated in the lipid bilayer morphologically indistinguishable from those formed on the native organism; and (D) the biological activity of said membrane protein is essentially preserved.
Claim 3
Dependent Claim
13 words
The composition of claim 1 wherein said membrane protein is a viral glycoprotein.
Claim 4
Dependent Claim
12 words
The composition of claim 2 essentially free of any unassociated viral glycoprotein.
Claim 5
Dependent Claim
11 words
The composition of claim 2 wherein said virus is Sendai virus.
Claim 6
Dependent Claim
12 words
The composition of claim 2 wherein said virus is influenza A virus.
Claim 7
Dependent Claim
28 words
A composition suitable for parenteral administration comprising a minor, effective amount of phospholipid vesicles of claim 1 and a major amount of a pharmaceutically acceptable parenteral carrier material.
Claim 8
Dependent Claim
13 words
The composition of claim 6 wherein said composition is useful as an immunogen.
Claim 9
Dependent Claim
33 words
A method for inducting immunity in a host which method comprises administering parenterally, on mucosal surfaces, or through the alimuntary tract, including administrative combinations thereof, to such host the composition of claim 7.
Claim 10
Dependent Claim
12 words
The method of claim 8 wherein said composition comprises a viral glycoprotein.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-05-04 | 1990-11-03 | 1991-05-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-05-04 | 1994-11-03 | 1995-05-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-05-04 | 1998-11-03 | 1999-05-05 | $7700.00 | Window Closed / Lapsed |