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US4661445A
Expired / Lapsed
US
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Competitive ELISA for the detection of HTLV-III antibodies
Assignee: United States Department of Health and Human Services
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Filing: 1985-05-24
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Granted: 1987-04-28
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Expires: 2005-05-24
Abstract
Abstract A competitive enzyme-linked immunosorbent assay (ELISA) for the detection of antibodies is disclosed. This ELISA technique is more sensitive, more specific, and more accurate than known ELISA techniques. The competitive ELISA of this invention is particularly suited to the detection of human T-cell leukemia-lymphoma virus type III (HTLV-III). Antigenic or viral fragments are bound to a solid support. The bound fragments are then incubated with a heterologous antiserum which binds to the bound fragments. Test sera is then added to the bound fragments and tested for absorbance.
Inventors
- 👤 W. Carl Saxinger
- 👤 Robert C. Gallo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 09:48
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; G01N33/56988; G01N33/543; G01N33/54393; Y; Y10; Y10S; Y10S435/00; Y10S435/81; Y10S435/974; Y10S435/975; Y10S436/00; Y10S436/807; Y10S436/808; Y10S436/809; Y10S436/811; Y10S436/825
Claims Analysis
Total Claims: 4
Claim 1
Independent Claim
72 words
A method for the detection of HTLV-III antibodies in a test sample comprising coating microtiter plates with purified HTLV-III antigen to form antigen-coated wells; incubating a portion of said antigen coated wells with heterologous anti-HTLV-III; adding test sera to said wells; screening said wells for the amount of binding of HTLV-III antibodies by comparing said wells containing said heterologous anti-HTLV-III to control wells, said control wells being incubated without said heterologous anti-THLV-III.
Claim 2
Independent Claim
72 words
A method for the detection of HTLV-III antibodies in a test sample comprising coating microtiter plates with purified HTLV-III antigen to form antigen-coated wells; incubating a portion of said antigen coated wells with heterologous anti-HTLV-III; adding test sera to said wells; screening said wells for the amount of binding of HTLV-III antibodies by comparing said wells containing said heterologous anti-HTLV-III to control wells, said control wells being incubated without said heterologous anti-THLV-III.
Claim 3
Dependent Claim
56 words
A test kit for the detection of HTLV-III antibodies comprising in combination an insoluble surface or support containing microtiter wells; HTLV-III fragments which are bound to said support; and heterologous antiserum which specifically binds to said HTLV-III fragments; and means for detecting the amount of antibodies in a test sample which bind to said HTLV-III fragments.
Claim 4
Dependent Claim
56 words
A test kit for the detection of HTLV-III antibodies comprising in combination an insoluble surface or support containing microtiter wells; HTLV-III fragments which are bound to said support; and heterologous antiserum which specifically binds to said HTLV-III fragments; and means for detecting the amount of antibodies in a test sample which bind to said HTLV-III fragments.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-04-27 | 1990-10-27 | 1991-04-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-04-27 | 1994-10-27 | 1995-04-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-04-27 | 1998-10-27 | 1999-04-28 | $7700.00 | Window Closed / Lapsed |