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US4640893A
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Novel rhodamine derivatives as fluorogenic substrates for proteinases
Assignee: University of Illinois at Chicago
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Filing: 1985-09-27
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Granted: 1987-02-03
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Expires: 2005-09-27
Abstract
Abstract The present invention relates to a new class of novel Rhodamine derivatives. These derivatives which are bisamide substitution products are nonfluorescent. Whereas the monoamide substitution products exhibit high fluorescence. Cleavage of a single specified amide bond by bond-specific proteinases will therefore convert the nonfluorescent bisamide derivative into a highly fluorescent monoamide derivative.
Inventors
- 👤 Walter F. Mangel
- 👤 Stephen Leytus
- 👤 L. Lee Melhado
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 17:01
CPC Classifications
C; C07; C07D; C07D493/00; C07D493/02; C07D493/10; C07K; C07K5/00; C07K5/04; C07K5/06; C07K5/06139; C07K5/06165; C09; C09B; C09B11/00; C09B11/04; C09B11/10; C09B11/24; Y; Y10; Y10S; Y10S436/00; Y10S436/80
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
65 words
A method of performing an enzymatic assay which comprises reacting a compound of the general formula ##STR7## wherein R 1 and R 2 , which are the same or different, are selected from the group consisting of amino acids, amino acid derivatives, blocked amino acids, blocked amino acid derivatives, lipophilic carbon containing compounds, and peptides with an enzyme capable of cleaving the amino-blocking group bond.
Claim 2
Independent Claim
65 words
A method of performing an enzymatic assay which comprises reacting a compound of the general formula ##STR7## wherein R 1 and R 2 , which are the same or different, are selected from the group consisting of amino acids, amino acid derivatives, blocked amino acids, blocked amino acid derivatives, lipophilic carbon containing compounds, and peptides with an enzyme capable of cleaving the amino-blocking group bond.
Claim 3
Dependent Claim
20 words
A method according to claim 1 wherein R 1 and R 2 are amino acids, blocked amino acids, or peptides.
Claim 4
Dependent Claim
14 words
A method according to claim 2 wherein R 1 and R 2 are peptides.
Claim 5
Dependent Claim
16 words
A method according to claim 3 wherein R 1 and R 2 are dipeptides and tripeptides.
Claim 6
Dependent Claim
30 words
A method according to claim 1 wherein R 1 and R 2 comprise a peptide in which the amino acid bound to the amino constituent of said formula is arginine.
Claim 7
Independent Claim
55 words
A method for attaching a blocked fluorogenic compound to a protein which comprises placing a compound of the formula ##STR8## wherein R 1 is blocking group and R 2 is an antigen-specific antibody in the presence of a protein which will react with the antigen-specific antibody, and allowing the antibody to react with the protein.
Claim 8
Independent Claim
55 words
A method for attaching a blocked fluorogenic compound to a protein which comprises placing a compound of the formula ##STR8## wherein R 1 is blocking group and R 2 is an antigen-specific antibody in the presence of a protein which will react with the antigen-specific antibody, and allowing the antibody to react with the protein.
Claim 9
Independent Claim
55 words
A method for attaching a blocked fluorogenic compound to a protein which comprises placing a compound of the formula ##STR9## wherein R 2 is blocking group and R 1 is an antigen-specific antibody in the presence of a protein which will react with the antigen-specific antibody, and allowing the antibody to react with the protein.
Claim 10
Independent Claim
55 words
A method for attaching a blocked fluorogenic compound to a protein which comprises placing a compound of the formula ##STR9## wherein R 2 is blocking group and R 1 is an antigen-specific antibody in the presence of a protein which will react with the antigen-specific antibody, and allowing the antibody to react with the protein.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1990-02-02 | 1990-08-04 | 1991-02-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1994-02-02 | 1994-08-04 | 1995-02-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1998-02-02 | 1998-08-04 | 1999-02-03 | $7700.00 | Window Closed / Lapsed |