US4626505A
Expired / Lapsed
US
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Selectable markers for yeast transformation
Assignee: EIDP Inc
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Filing: 1984-02-24
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Granted: 1986-12-02
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Expires: 2004-02-24
Abstract
Abstract Plasmid vectors capable of transforming Saccharomyces cerevisiae to phenotypes characterized by resistance to selective concentrations of sulfonylurea herbicides are disclosed.
Inventors
- 👤 Saverio C. Falco
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 00:24
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/945
Claims Analysis
Total Claims: 33
Claim 1
Independent Claim
37 words
A plasmid vector adapted for transformation of a host strain of qenus Saccharomyces, said vector comprising a first DNA segment capable of transforminq sensitive cells to a phenotype characterized by resistance to selective concentrations of sulfometuron methyl.
Claim 2
Dependent Claim
37 words
A plasmid vector adapted for transformation of a host strain of qenus Saccharomyces, said vector comprising a first DNA segment capable of transforminq sensitive cells to a phenotype characterized by resistance to selective concentrations of sulfometuron methyl.
Claim 3
Dependent Claim
14 words
A plasmid vector according to claim 1, wherein the host strain is Saccharomvces cerevisiae.
Claim 4
Dependent Claim
14 words
A plasmid vector according to claim 2, wherein said vector is a multicopy plasmid.
Claim 5
Dependent Claim
26 words
A plasmid vector according to claim 3, wherein the first DNA segment comprises at least one deoxyribonucleotide sequence corresponding to a wild-type Saccharomyces cerevisiae ILV2 gene.
Claim 6
Dependent Claim
38 words
A plasmid vector according to claim 2, wherein the first DNA segment comprises a deoxyribonucleotide sequence allelic to a Saccharomvces cerevisiae gene, which sequence encodes a gene product capable of conferring resistance to selective concentrations of sulfometuron methyl.
Claim 7
Dependent Claim
24 words
A plasmid vector according to claim 5, wherein the sequence is a mutant allele of ILV2, a S. cerevisiae structural gene encoding acetolactate synthase.
Claim 8
Dependent Claim
21 words
A plasmid vector according to claim 6, wherein the mutant allele is dominant with respect to a wild-type S. cerevisiae gene.
Claim 9
Dependent Claim
15 words
A plasmid vector according to claim 7, additionally comprising plasmid DNA derived from S. cerevisiae.
Claim 10
Dependent Claim
14 words
A plasmid vector according to claim 8, additionally comprising a yeast origin of replication.
Claim 11
Dependent Claim
14 words
A plasmid vector according to claim 9, additionally comprising a bacterial origin of replication.
Claim 12
Dependent Claim
17 words
A plasmid vector according to claim 10, wherein the bacterial origin of replication is derived from pBR322.
Claim 13
Dependent Claim
16 words
A plasmid vector accordinq to claim 11, wherein said plasmid vector is derived from plasmid YEp24.
Claim 14
Dependent Claim
9 words
A plasmid vector according to claim 12, plasmid pCP2-4-10.
Claim 15
Dependent Claim
16 words
A plasmid vector according to claim 13, wherein the host strain is S. cerevisiae ATCC 7754.
Claim 16
Dependent Claim
20 words
A plasmid vector according to claim 9, wherein the yeast origin of replication is derived from an endogenous yeast plasmid.
Claim 17
Dependent Claim
22 words
A plasmid vector according to claim 8, additionally comprising a second DNA segment comprising a deoxyribonucleotide sequence normally exogenous to S. cerevisiae.
Claim 18
Dependent Claim
14 words
A plasmid vector according to claim 5, wherein the selective concentration is 3 μg/mL.
Claim 19
Dependent Claim
14 words
A plasmid vector according to claim 17, wherein the selective concentration is 30 μg/mL.
Claim 20
Dependent Claim
31 words
A plasmid vector/host strain system for expression of DNA normally exogenous to S. cerevisiae, said system comprising a suitable S. cerevisiae host strain and a plasmid vector according to claim 5.
Claim 21
Dependent Claim
24 words
A plasmid vector/host strain system according to claim 19, wherein the host strain is S. cerevisiae ATCC 7754 and the plasmid vector is pCP2-4-10.
Claim 22
Dependent Claim
12 words
A transformant S. cerevisiae cell comprising a plasmid according to claim 5.
Claim 23
Dependent Claim
13 words
A transformant S. cerevisiae cell cekkm comprising a plasmid according to claim 1.
Claim 24
Dependent Claim
63 words
A method of introducing DNA normally exogenous to S. cerevisiae to a S. cerevisiae host cell, comprising: (a) transforming a suitable host strain population with a plasmid vector of claim 1; (b) exposing said population to a selective concentration of sulfometuron methyl or other sulfonylurea herbicide; and (c) selecting transformants capable of growth in the presence of the selective concentration of the herbicide.
Claim 25
Dependent Claim
16 words
A method according to claim 23, wherein the plasmid vector is as defined in claim 5.
Claim 26
Dependent Claim
21 words
A method according to claim 24, wherein the sulfonylurea growth inhibitor is sulfometuron methyl and the selective concentration is 30 μg/mL.
Claim 27
Dependent Claim
13 words
A method according to claim 25, wherein the plasmid vector is plasmid pCP2-4-10.
Claim 28
Dependent Claim
26 words
A DNA segment comprising a deoxyribonucleotide sequence allelic to a Saccharomyces cerevisiae gene, which sequence encodes a gene product resistant to selective concentrations of sulfometuron methyl.
Claim 29
Dependent Claim
26 words
A DNA segment comprising a deoxyribonucleotide sequence allelic to a Saccharomyces cerevisiae gene, which sequence encodes a gene product resistant to selective concentrations of sulfometuron methyl.
Claim 30
Dependent Claim
24 words
A DNA segment according to claim 27, wherein the sequence is a mutant allele of ILV2, a S. cerevisiae structural gene encoding acetolactate synthase.
Claim 31
Dependent Claim
13 words
A DNA segment according to claim 28, wherein the sequence is fragment CP2-4-10.
Claim 32
Dependent Claim
8 words
A transformant S. cerevisiae cell comprising plasmid pCP2-4-10.
Claim 33
Dependent Claim
8 words
A transformant S. cerevisiae cell comprising plasmid pCP2-4-10.
References Cited (Backward Citations)
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| 3.5 Years (Year 4) | 1989-12-01 | 1990-06-02 | 1990-12-02 | $2000.00 | Window Closed / Lapsed |
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