US4511652A
Expired / Lapsed
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High efficiency eukaryotic metallothionein promoter system
Assignee: University of California
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Filing: 1982-06-03
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Granted: 1985-04-16
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Expires: 2002-06-03
Abstract
Abstract Yeast metallothionein (copper chelatin) and DNA sequences having the gene encoding the polypeptide are provided. The DNA sequences find use in producing copper chelatin and in amplifying downstream flanking regions.
Inventors
- 👤 Seymour Fogel
- 👤 Juliet W. Welch
- 👤 Michael Karin
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 05:07
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/09; C12N15/63; C12N15/67; C07; C07K; C07K14/00; C07K14/825; C12N15/79; C12N15/80; C12N15/81
Claims Analysis
Total Claims: 23
Claim 1
Independent Claim
26 words
A DNA sequence encoding yeast copper chelatin or a copper chelating fragment thereof having naturally occurring flanking regions, each flanking region being less than 5 kbp.
Claim 2
Dependent Claim
26 words
A DNA sequence encoding yeast copper chelatin or a copper chelating fragment thereof having naturally occurring flanking regions, each flanking region being less than 5 kbp.
Claim 3
Dependent Claim
25 words
A DNA sequence according to claim 1, comprising the naturally occurring yeast expression regulatory signals for expression upstream from the sequence encoding yeast copper chelatin.
Claim 4
Dependent Claim
37 words
A DNA sequence according to claim 2, comprising regulatory signals for expression recognized by the yeast host and a gene encoding a polypeptide foreign to a yeast host downstream from said gene encoding said yeast copper chelatin.
Claim 5
Dependent Claim
34 words
A DNA sequence according to claim 3, wherein said gene encoding said yeast copper chelatin and said foreign gene and their regulatory signals encompass a total number of nucleotides of less than 2 kbp.
Claim 6
Dependent Claim
20 words
A multi-copy DNA sequence having tandem iterations of a DNA sequence according to any of claims 1, 2, or 4.
Claim 7
Dependent Claim
57 words
A DNA sequence comprising a pair of expression regulatory signals recognized by a yeast host separated by a gene encoding yeast copper chelatin and a foreign gene under the regulatory control and downstream from the downstream regulatory signals and having a flanking sequence of at least 100 bp substantially homologous to a portion of a yeast chromosome.
Claim 8
Dependent Claim
57 words
A DNA sequence comprising a pair of expression regulatory signals recognized by a yeast host separated by a gene encoding yeast copper chelatin and a foreign gene under the regulatory control and downstream from the downstream regulatory signals and having a flanking sequence of at least 100 bp substantially homologous to a portion of a yeast chromosome.
Claim 9
Dependent Claim
26 words
A DNA sequence according to claim 6, wherein said flanking region is homologous to a chromosomal portion coding for an enzyme involved in a metabolic process.
Claim 10
Dependent Claim
29 words
An extrachromosomal construct comprising a yeast replicon, regulatory signals recognized by yeast for expression and under the control of said regulatory signals, a gene encoding for yeast copper chelatin.
Claim 11
Dependent Claim
29 words
An extrachromosomal construct comprising a yeast replicon, regulatory signals recognized by yeast for expression and under the control of said regulatory signals, a gene encoding for yeast copper chelatin.
Claim 12
Dependent Claim
68 words
An extrachromosomal construct according to claim 8, comprising: (a) second expression regulatory signals recognized by yeast, said second expression regulatory signals being downstream from said yeast copper chelatin gene; and (b) a gene encoding a polypeptide foreign to yeast under the control of said second regulatory signals, wherein said foreign gene is less than 1 kb from the terminal codon of said gene encoding for yeast copper chelatin.
Claim 13
Dependent Claim
25 words
An extrachromosomal construct according to claim 9, wherein said regulatory signals and said two genes encompass a DNA segment of not more than 2 kbp.
Claim 14
Dependent Claim
22 words
An extrachromosomal construct according to any of claims 9 or 10, wherein said regulatory genes and said two genes are tandemly iterated.
Claim 15
Dependent Claim
15 words
An extrachromosomal construct according to claim 11, wherein said iteration is at least 15 units.
Claim 16
Dependent Claim
16 words
A yeast cell having an extrachromosomal construct according to any of claims 8, 9 or 10.
Claim 17
Dependent Claim
58 words
A yeast cell having iterated DNA sequences of: (a) first expression regulatory signals; (b) a gene encoding for yeast copper chelatin; (c) second expression regulatory signals; and (d) a gene foreign to said yeast cell, wherein said genes are under the regulatory control of the proximal regulatory signals and said regulatory signals are recognized by said yeast cell.
Claim 18
Dependent Claim
58 words
A yeast cell having iterated DNA sequences of: (a) first expression regulatory signals; (b) a gene encoding for yeast copper chelatin; (c) second expression regulatory signals; and (d) a gene foreign to said yeast cell, wherein said genes are under the regulatory control of the proximal regulatory signals and said regulatory signals are recognized by said yeast cell.
Claim 19
Dependent Claim
23 words
A yeast cell according to claim 14 wherein at least a portion of said iterated sequences are present on an extrachromosomal DNA element.
Claim 20
Dependent Claim
21 words
A yeast cell according to claim 14, wherein at least a portion of said iterated sequences are present in a chromosome.
Claim 21
Dependent Claim
17 words
A yeast cell according to any of claims 14, 15 or 16 having at least 15 iterations.
Claim 22
Dependent Claim
55 words
In a method for detecting yeast cells modified by introduction of a DNA segment containing a gene capable of expression in said yeast host; the improvement which comprises having as part of said DNA segment, a gene expressing yeast copper chelatin capable of expression in said yeast; and selecting for yeast cells resistant to copper.
Claim 23
Dependent Claim
55 words
In a method for detecting yeast cells modified by introduction of a DNA segment containing a gene capable of expression in said yeast host; the improvement which comprises having as part of said DNA segment, a gene expressing yeast copper chelatin capable of expression in said yeast; and selecting for yeast cells resistant to copper.
References Cited (Backward Citations)
US4387162A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-15 | 1988-10-15 | 1989-04-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-15 | 1992-10-15 | 1993-04-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-15 | 1996-10-15 | 1997-04-16 | $7700.00 | Window Closed / Lapsed |