Patent US1108027A successfully refreshed from Google Patents.
US4387162A
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Hybrid plasmids and microorganisms containing them
Assignee: Bpifrance Financement SA
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Filing: 1979-11-14
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Granted: 1983-06-07
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Expires: 1999-11-14
Abstract
Abstract A hybrid plasmid comprising at least the DNA of a bacterial plasmid, all or part of the DNA of the 2μ plasmid of yeast and a segment of DNA including the gene URA 3 + of yeast. These plasmids are useful as vectors of exogenous DNA in yeasts.
Inventors
- 👤 Michel Aigle
- 👤 Hughes Blanc
- 👤 Philippe Fournier
- 👤 Claude Gerbaud
- 👤 Michel Guerineau
- 👤 Henri Heslot
- 👤 Francois Lacroute
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 11:24
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/88; C12N15/00; C12N15/09; C12N15/63; C12N15/79; C12N15/80; C12N15/81
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
47 words
A hybrid plasmid comprising a DNA of a bacterial plasmid, all or part of the DNA of a 2μ plasmid of yeast, and a DNA segment of about 1.1 kb including the gene URA 3 + of yeast which is limited by two Hind III restriction sites.
Claim 2
Dependent Claim
47 words
A hybrid plasmid comprising a DNA of a bacterial plasmid, all or part of the DNA of a 2μ plasmid of yeast, and a DNA segment of about 1.1 kb including the gene URA 3 + of yeast which is limited by two Hind III restriction sites.
Claim 3
Dependent Claim
30 words
A hybrid plasmid according to claim 1, wherein the DNA segment, including the gene URA 3 + of yeast, is inserted in the DNA of the 2μ plasmid of yeast.
Claim 4
Dependent Claim
55 words
A hybrid plasmid according to claim 2, wherein the DNA segment including the gene URA 3 + of yeast, is inserted in the DNA of the 2μ plasmid of yeast, between the restriction sites Hind III (1) and Hind III (3), with corresponding loss of segment of the DNA of the 2μ plasmid of yeast.
Claim 5
Dependent Claim
28 words
A hybrid plasmid according to claim 1, wherein the DNA segment including the gene URA 3 + of yeast is inserted in the DNA of the bacterial plasmid.
Claim 6
Dependent Claim
24 words
A hybrid plasma according to claim 2 wherein the DNA of the bacterial plasmid includes an insertion of a DNA of a eucaryote organism.
Claim 7
Dependent Claim
38 words
A hybrid plasmid according to claim 4, wherein the DNA of the bacterial plasmid includes the insertion of a DNA of a eucaryote organism as well as the DNA segment including the gene URA 3 + of yeast.
Claim 8
Dependent Claim
23 words
A hybrid plasmid according to claim 1 wherein the DNA of bacterial plasmid is the DNA of the plasmid pCR1 or pBR 322.
Claim 9
Dependent Claim
30 words
A plasmid according to claim 1 wherein the DNA of the 2μ plasmid of yeast is inserted in the DNA of the bacterial plasmid between the Eco R1 restrictions sites.
Claim 10
Dependent Claim
14 words
A microorganism including a hybrid plasmid according to any of claims 1 to 8.
Claim 11
Dependent Claim
6 words
A yeast according to claim 9.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-06-06 | 1986-12-06 | 1987-06-07 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-06-06 | 1990-12-06 | 1991-06-07 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-06-06 | 1994-12-06 | 1995-06-07 | $7700.00 | Window Closed / Lapsed |