US4623623A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for producing L-lysine by fermentation
Assignee: KH Neochem Co Ltd
•
Filing: 1985-06-03
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Granted: 1986-11-18
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Expires: 2005-06-03
Abstract
Abstract A process is disclosed for producing L-lysine by culturing a microorganism obtained by protoplast fusion and having an ability to produce L-lysine in a nutrient medium, forming and accumulating L-lysine in the resulting culture liquor, and recovering the L-lysine therefrom. Also, there are disclosed a number of microorganisms for producing L-lysine which are obtained by protoplast fusion.
Inventors
- 👤 Toshihide Nakanishi
- 👤 Tomoki Azuma
- 👤 Toshihiko Hirao
- 👤 Kiyoji Hattori
- 👤 Minoru Sakurai
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 08:45
CPC Classifications
C; C12; C12N; C12N15/00; C12N15/02; C12N15/03; C12P; C12P13/00; C12P13/04; C12P13/08; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/84; Y10S435/843
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
78 words
A process for producing L-lysine, which comprises culturing a protoplast fusion strain H-3057 (FERM BP-148) obtained by protoplast fusion between Corynebacterium glutamicum H-3122 (FERM BP-150) and Brevibacterium lactofermentum H-3126 (FERM BP-152), a protoplast fusion strain H-3055 (FERM BP-147) obtained by protoplast fusion between Corynebacterium glutamicum H-3122 (FERM BP-150) and Corynebacterium glutamicum H-3119 (FERM BP-149), or Corynebacterium glutamicum H-3149 (FERM BP-158) in a nutrient medium, forming and accumulating L-lysine in the resulting culture liquor, and recovering the L-lysine therefrom.
Claim 2
Dependent Claim
78 words
A process for producing L-lysine, which comprises culturing a protoplast fusion strain H-3057 (FERM BP-148) obtained by protoplast fusion between Corynebacterium glutamicum H-3122 (FERM BP-150) and Brevibacterium lactofermentum H-3126 (FERM BP-152), a protoplast fusion strain H-3055 (FERM BP-147) obtained by protoplast fusion between Corynebacterium glutamicum H-3122 (FERM BP-150) and Corynebacterium glutamicum H-3119 (FERM BP-149), or Corynebacterium glutamicum H-3149 (FERM BP-158) in a nutrient medium, forming and accumulating L-lysine in the resulting culture liquor, and recovering the L-lysine therefrom.
Claim 3
Dependent Claim
21 words
The process according to claim 1, wherein said culturing is conducted at 20° to 40° C. for 1 to 6 days.
Claim 4
Dependent Claim
95 words
A biologically pure culture of a protoplast fusion strain H-3057 (FERM BP-148) having a resistance to streptomycin and rifampicin obtained by protoplast fusion between Corynebacterium glutamicum H-3122 (FERM BP-150) and Brevibacterium lactofermentum H-3126 (FERM BP-152), a protoplast fusion strain H-3055 (FERM BP-147) having a resistance to streptomycin and rifampicin obtained by protoplast fusion between Corynebacterium glutamicum H-3122 (FERM BP-150) and Corynebacterium glutamicum H-3119 (FERM BP-149), or a microorganism strain H-3149 (FERM BP-158) having a resistance to streptomycin, rifampicin and 6-azauracil derived from the protoplast fusion strain H-3055, which culture possesses the ability to produce L-lysine.
Claim 5
Dependent Claim
95 words
A biologically pure culture of a protoplast fusion strain H-3057 (FERM BP-148) having a resistance to streptomycin and rifampicin obtained by protoplast fusion between Corynebacterium glutamicum H-3122 (FERM BP-150) and Brevibacterium lactofermentum H-3126 (FERM BP-152), a protoplast fusion strain H-3055 (FERM BP-147) having a resistance to streptomycin and rifampicin obtained by protoplast fusion between Corynebacterium glutamicum H-3122 (FERM BP-150) and Corynebacterium glutamicum H-3119 (FERM BP-149), or a microorganism strain H-3149 (FERM BP-158) having a resistance to streptomycin, rifampicin and 6-azauracil derived from the protoplast fusion strain H-3055, which culture possesses the ability to produce L-lysine.
References Cited (Backward Citations)
US2979439A
Cited
US3707441A
Cited
US3687810A
Cited
US3905867A
Cited
US3825472A
Cited
US3871960A
Cited
US3929571A
Cited
AU487641B2
Cited
US4066501A
Cited
US4169763A
Cited
US4172764A
Cited
US4275157A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-11-17 | 1990-05-19 | 1990-11-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-11-17 | 1994-05-19 | 1994-11-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-11-17 | 1998-05-19 | 1998-11-18 | $7700.00 | Window Closed / Lapsed |