US3929571A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for preparing L-lysine
Assignee: Ajinomoto Co Inc
•
Filing: 1974-05-20
•
Granted: 1975-12-30
•
Expires: 1994-05-20
Abstract
Abstract Better yields of L-lysine can be obtained by culturing an Llysine producing microorganism in a nutrient medium supplememted with an antibiotic, a surface active agent and/or an antioxidant.
Inventors
- 👤 Koji Kubota
- 👤 Yasuhiko Yoshihara
- 👤 Yoshio Hirose
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 12:10
CPC Classifications
C; C12; C12P; C12P13/00; C12P13/04; C12P13/08; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/83; Y10S435/84; Y10S435/843; Y10S435/859; Y10S435/861
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
120 words
IN A PROCESS OF PRODUCING L-LYSINE BY AEROBICALLY CULTURING AN L-LYSINE PRODUCING MICROORGANISM AT PH 5 TO 9 ON A MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN, INORGANIC SALTS AND MONOR ORGANIC NUTRIENTS REQUIRED FOR THE GROWTH OF SAID MICROORGANISM UNTIL L-LYSINE ACCUMULATES IN SAID MEDIUM, AND BY RECOVERING THE ACCUMULATED L-LYSINE, SAID MICROORGANISM BEING A STRAIN OF BREVIBACTERIUM, CORYNEBACTERIUM, MICROBACTERIUM, MICROCOCCUS, OR ARTHROBACTER, THE IMPRIVEMENT WHICH COMPRISES CULTURING SAID MUCROORGANISM IN SAID MEDIUM IN THE PRESENCE OF AN AMOUNT OF AN ADDITION AGENT SUFFICIENT TO IMPROVE THE YIELD OF SAID L-LYSINE AS COMPARED TO THE YIELD OF SAID L-LYSINE PRODUCED IN THE ABSENCE OF SAID AGENT, SAID AGENT BEING AN ANTIBIOTIC, A SURFACE ACTIVE AGENT, OR AN ANTI-OXIDANT.
Claim 2
Dependent Claim
120 words
IN A PROCESS OF PRODUCING L-LYSINE BY AEROBICALLY CULTURING AN L-LYSINE PRODUCING MICROORGANISM AT PH 5 TO 9 ON A MEDIUM CONTAINING ASSIMILABLE SOURCES OF CARBON AND NITROGEN, INORGANIC SALTS AND MONOR ORGANIC NUTRIENTS REQUIRED FOR THE GROWTH OF SAID MICROORGANISM UNTIL L-LYSINE ACCUMULATES IN SAID MEDIUM, AND BY RECOVERING THE ACCUMULATED L-LYSINE, SAID MICROORGANISM BEING A STRAIN OF BREVIBACTERIUM, CORYNEBACTERIUM, MICROBACTERIUM, MICROCOCCUS, OR ARTHROBACTER, THE IMPRIVEMENT WHICH COMPRISES CULTURING SAID MUCROORGANISM IN SAID MEDIUM IN THE PRESENCE OF AN AMOUNT OF AN ADDITION AGENT SUFFICIENT TO IMPROVE THE YIELD OF SAID L-LYSINE AS COMPARED TO THE YIELD OF SAID L-LYSINE PRODUCED IN THE ABSENCE OF SAID AGENT, SAID AGENT BEING AN ANTIBIOTIC, A SURFACE ACTIVE AGENT, OR AN ANTI-OXIDANT.
Claim 3
Dependent Claim
23 words
In a process as set forth in claim 1, the amount of said agent being sufficient to inhibit the growth of said microorganism.
Claim 4
Dependent Claim
15 words
In a process as set forth in claim 1, said microorganism being resistant to S-(2-aminoethyl)-cysteine.
Claim 5
Dependent Claim
42 words
In a process as set forth in claim 1, said microorganism requiring at least one nutrient selected from the group consisting of homoserine, threonine, methionine, alanine, proline, serine, isoleucine, valine, leucine, arginine, histidine, nicotinamide, panthothenic acid, thiamine, guanine, hypoxanthine and vitamine B12.
Claim 6
Dependent Claim
40 words
In a process as set forth in claim 1, said agent being selected from the group consisting of chloramphenicol, erythromycin, oleandomycin, kanamycin, streptomycin, kasugamycin, tetracycline, oxytetracycline, mytomycin C, actinomycin D, cycloserine, penicillin group compounds, cephalosporin group compounds, polymyxin and azaserine.
Claim 7
Dependent Claim
51 words
In a process as set forth in claim 1, said agent being a surface active agent selected from the group consisting of a sulfate of a higher alcohol, alkylbenzenesulfonate, alkyl phosphate, dialkyl sulfosuccinate, an alkylamine salt, a quaternary ammonium salt, polyoxyethylene alkyl ether, polyoxyethylene sorbitan monoalkylether, sorbitan monolaurate, imidazoline and betaine.
Claim 8
Dependent Claim
50 words
In a process as set forth in claim 1, said agent being an antioxidant selected from the group consisting of 4,4''-dihydroxy-3,3''-dimethyldiphenyl, 2,6-di-3-butylphenol, catechol, butylcatechol, protocatechuic acid, Alpha -tocopherol, pyrogallol, gallic acid, an ester of gallic acid, naphthol, aminophenol, naphthylamine, diphenylamine, di-2-butyl-p-phenylenediamine, 6-ethyoxy-2,3,4-trimethyl-1,2-dihydroquinoline, semi-carbazide, phenothiazine, tetraphenylhydrazine, thio-dipropionic acid and thio-diglycolic acid.
References Cited (Backward Citations)
US3707441A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-29 | 1979-06-30 | 1979-12-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-29 | 1983-06-30 | 1983-12-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-29 | 1987-06-30 | 1987-12-30 | $7700.00 | Window Closed / Lapsed |