Patent US4613569A successfully refreshed from Google Patents.
US4613569A
Expired / Lapsed
US
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Stabilized composition of tetrazolium salts
Assignee: Roche Diagnostics GmbH
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Filing: 1984-03-27
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Granted: 1986-09-23
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Expires: 2004-03-27
Abstract
Abstract The present invention provides stabilized compositions of tetrazolium salts for analytical purposes, wherein they contain 1 to 10 moles of a complex-forming acid which is soluble in polar solvents per mole of tetrazolium salt. The present invention also provides a process for the detection of reducing materials, wherein one of the above stabilized compositions is added to a test batch.
Inventors
- 👤 Edda Geisler
- 👤 Helmut Feuerstein
- 👤 Hans Lange
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 23:06
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/52; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81; Y10S436/00; Y10S436/903; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444; Y10T436/145555; Y10T436/146666; Y10T436/20; Y10T436/200833; Y10T436/201666; Y10T436/25; Y10T436/2525
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
78 words
Method for the detection of reducing materials by means of tetrazolium salts which method comprises adding a tetrazolium salt stabilized by the addition of 1 to 10 moles of a complex-forming acid selected from the group consisting of boric acid and an organic hydroxypolycarboxylic acid which is soluble in polar solvents, per mole of tetrazolium salts, to a solution buffered to the reaction conditions of the other reagents; subsequently adding the sample thereto and measuring the color change.
Claim 2
Dependent Claim
78 words
Method for the detection of reducing materials by means of tetrazolium salts which method comprises adding a tetrazolium salt stabilized by the addition of 1 to 10 moles of a complex-forming acid selected from the group consisting of boric acid and an organic hydroxypolycarboxylic acid which is soluble in polar solvents, per mole of tetrazolium salts, to a solution buffered to the reaction conditions of the other reagents; subsequently adding the sample thereto and measuring the color change.
Claim 3
Dependent Claim
17 words
Method as claimed in claim 1, further comprising adding diaphorase as an activator for the tetrazolium salts.
Claim 4
Dependent Claim
17 words
Method as claimed in claim 1, further comprising adding PMS as an activator for the tetrazolium salts.
Claim 5
Dependent Claim
23 words
The method as claimed in claim 1 wherein 1 to 2 moles of the complex-forming acid is used per mole of tetrazolium salt.
Claim 6
Dependent Claim
14 words
The method as claimed in claim 1, wherein the complex-forming acid is boric acid.
Claim 7
Dependent Claim
17 words
The method as claimed in claim 1, wherein said complex forming acid is an organic hydroxypolycarboxylic acid.
Claim 8
Dependent Claim
15 words
The method as claimed in claim 1, wherein the complex forming acid is citric acid.
Claim 9
Dependent Claim
14 words
The method as claimed in claim 1, wherein the tetrazolium salt is 3-(4',5'-dimethylthiazolyl-2)-2,4-diphenyltetrazolium bromide.
Claim 10
Dependent Claim
14 words
The method as claimed in claim 1, wherein the tetrazolium salt is 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride.
Claim 11
Dependent Claim
14 words
The method as claimed in claim 1, wherein the tetrazolium salt is 2,2'-di-(p-nitrophenyl)-5,5'-diphenyl-3,3'-(3,3'-dimethoxy-4,4'-diphenylene)-ditetrazolium chloride.
Claim 12
Dependent Claim
18 words
The method as claimed in claim 1, wherein the stabilized composition is in the form of a solution.
Claim 13
Dependent Claim
14 words
The method as claimed in claim 1, wherein the stabilized composition is a lyophilizate.
Claim 14
Dependent Claim
17 words
The method as claimed in claim 1, wherein the stabilized composition is impregnated into an absorbent carrier.
References Cited (Backward Citations)
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-09-22 | 1990-03-24 | 1990-09-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-09-22 | 1994-03-24 | 1994-09-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-09-22 | 1998-03-24 | 1998-09-23 | $7700.00 | Window Closed / Lapsed |