US3732147A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Colorimetric determination of dehydrogenases
Assignee: Bayer Corp
•
Filing: 1971-09-16
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Granted: 1973-05-08
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Expires: 1991-09-16
Abstract
Abstract Test composition and method for assaying dehydrogenase enzymes in test fluids which comprises contacting the fluid with a test reagent comprising a coenzyme, a substrate for the dehydrogenase enzyme, a terminal acceptor dye and Meldola''s Blue as an intermediary hydrogen carrier. A preferable embodiment of the present invention comprises incorporating the test composition with a solid carrier therefor and using the same as a dip and read test device.
Inventors
- 👤 A Fosker
- 👤 P Mill
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 08:10
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
28 words
A composition according to claim 1 wherein the terminal acceptor dye is selected from the group consisting of 2-(p-iodophenyl)-3(p-nitrophenyl)-5-phenyl tetrazolium chloride 2-(p-iodophenyl)-3(p-nitrophenyl)-5-phenyl tetrazolium bromide and 2-(p-iodophenyl)-3(p-nitrophenyl)-5-phenyl tetrazolium iodide.
Claim 2
Dependent Claim
20 words
A composition according to claim 1 wherein the enzyme being determined is lactic dehydrogenase and the substrate is lactic acid.
Claim 3
Dependent Claim
19 words
A composition according to claim 1 wherein the enzyme being determined is glucose-6-phosphate dehydrogenase and the substrate is glucose-6-phosphate.
Claim 4
Dependent Claim
19 words
A composition according to claim 1 wherein the enzyme being determined is 6-phosphogluconate dehydrogenase and the substrate is 6-phosphogluconate.
Claim 5
Dependent Claim
22 words
A test device for the determination of a dehydrogenase enzyme comprising a carrier with which is incorporated the composition of claim 1.
Claim 6
Dependent Claim
14 words
A test device according to claim 6 wherein the carrier is a bibulous paper.
Claim 7
Independent Claim
57 words
A method for the determination of a dehydrogenase enzyme, which comprises contacting the fluid containing the enzyme with a predetermined proportion of a composition comprising a coenzyme selected from the group consisting of dinucleotide and nicotinamide adenine dinucleotide phosphate, a corresponding substrate for said enzyme, a terminal acceptor dye and Meldola''s Blue as an intermediary hydrogen carrier.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-05-07 | 1976-11-06 | 1977-05-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-05-07 | 1980-11-06 | 1981-05-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-05-07 | 1984-11-06 | 1985-05-08 | $7700.00 | Window Closed / Lapsed |