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US4609707A
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US
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Synthesis of polymers containing integral antibodies
Assignee: Genetic Systems Corp
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Filing: 1984-11-07
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Granted: 1986-09-02
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Expires: 2004-11-07
Abstract
Abstract A method is disclosed for the de novo synthesis of antibody-containing polymers and the preparation of a class of polymerizable compounds used in the synthesis of such antibody-containing polymers. Antibody-containing polymers formed from monomer/antibody conjugates and nonderivatized polymerizable compounds can be varied in formation of the polymer to provide control of (a) molecular spacing, steric accessibility and the number of antibody molecules that are integrally incorporated into the polymer backbone, and (b) the chemical and physical structure of the polymer itself, thus enabling specific tailoring of antibody-containing polymers for particular end-use application. Also disclosed is a method for the selective removal of a compound from a solution or suspension thereof using monomer/receptor conjugates where the compound has the capacity to bind to the receptor in the conjugate. The bound compound is removed by polymerization - induced separation of the monomer/receptor--compound complex from solution.
Inventors
- 👤 Robert C. Nowinski
- 👤 Allan S. Hoffman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 11:13
CPC Classifications
C; C08; C08F; C08F290/00; C08F290/08; C08F290/14; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G01N2650/00; Y; Y10; Y10S; Y10S436/00; Y10S436/827; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/817
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
37 words
A method for the de novo synthesis of antibody-containing polymers, comprising: forming a monomer/antibody conjugate with the antibody covalently bonded to the monomer, and initiating polymerization of the monomer/antibody conjugate to form polymers integrally containing the antibody.
Claim 2
Independent Claim
37 words
A method for the de novo synthesis of antibody-containing polymers, comprising: forming a monomer/antibody conjugate with the antibody covalently bonded to the monomer, and initiating polymerization of the monomer/antibody conjugate to form polymers integrally containing the antibody.
Claim 3
Dependent Claim
24 words
The method of claim 1 wherein the monomer is bonded to a chemical intermediate "spacer arm" compound which is also bonded to the antibody.
Claim 4
Independent Claim
22 words
The method of claims 1 or 2 wherein polymerization is initiated with nonderivatized polymerizable compounds to form copolymers integrally containing the antibody.
Claim 5
Dependent Claim
25 words
The method of claim 1 wherein the antibody is selected from the group consisting of naturally occurring, monoclonal, polyclonal, chemically synthesized and recombinant DNA (rDNA)-derived.
Claim 6
Dependent Claim
18 words
The method of claim 1 wherein the monomer/antibody conjugate is soluble in water or water/polar organic solvent mixtures.
Claim 7
Independent Claim
34 words
The method of claims 1 or 2 wherein the monomer is a compound containing olefinic or acetylenic unsaturation and at least one reactive site for bonding with the antibody or intermediate "spacer arm" compound.
Claim 8
Independent Claim
31 words
The method of claims 1 or 2 wherein the monomer is a polyunsaturated, polymerizable oligomer having at least one reactive site for bonding with the antibody or intermediate "spacer arm" compound.
Claim 9
Dependent Claim
29 words
The method of claim 3 wherein the nonderivatized polymerizable compounds are selected from the group consisting of olefinically unsaturated compounds, acetylenically unsaturated compounds, polyunsaturated compounds and multi-functional cross-linking compounds,
Claim 10
Dependent Claim
35 words
A conjugate used in the de novo preparation of polymers containing one or more antibodies, comprising: a monomer containing at least one reactive site for bonding with an antibody; and an antibody convalently bonded thereto.
Claim 11
Dependent Claim
35 words
A conjugate used in the de novo preparation of polymers containing one or more antibodies, comprising: a monomer containing at least one reactive site for bonding with an antibody; and an antibody convalently bonded thereto.
Claim 12
Dependent Claim
25 words
The conjugate of claim 9 wherein the antibody is selected from the group consisting of naturally occurring monoclonal, polyclonal, chemically synthesized and recombinant DNA (rDNA)-derived.
Claim 13
Dependent Claim
23 words
The conjugate of claim 9 wherein the antibody is bonded to an intermediate "spacer arm" compound which is also bonded to the monomer.
Claim 14
Dependent Claim
17 words
The conjugate of claim 9 wherein the monomer is soluble in water or water/polar organic solvent mixtures.
Claim 15
Dependent Claim
16 words
The conjugate of claims 9 or 11 homopolymerized to form a polymer integrally containing the antibody.
Claim 16
Dependent Claim
40 words
The polymer or copolymer of claim 13 wherein the polymer is soluble in water or water/polar organic solvent mixtures, and capable of being formed into shaped solids, films, particles, tubes, fibers, coatings, gels, and filters, upon removal of the water.
Claim 17
Dependent Claim
28 words
The polymer or copolymer of claim 13 wherein the polymer is water-insoluble and capable of being formed into shaped solids, films, particles, tubes, fibers, coatings, gels and filters.
Claim 18
Dependent Claim
19 words
The conjugate of claims 9 or 11 copolymerized with nonderivatized monomer to form a copolymer integrally containing the antibody.
Claim 19
Dependent Claim
34 words
The conjugate of claims 9 or 11 wherein the monomer is a compound containing olefinic or acetylenic unsaturation and at least one reactive site for bonding with the antibody or intermediate "spacer arm" compound.
Claim 20
Dependent Claim
40 words
The polymer or copolymer of claim 16 wherein the polymer is soluble in water or water/polar organic solvent mixtures, and capable of being formed into shaped solids, films, particles, tubes, fibers, coatings, gels, and filters, upon removal of the water.
Claim 21
Dependent Claim
28 words
The polymer or copolymer of claim 16 wherein the polymer is water-insoluble and capable of being formed into shaped solids, films, particles, tubes, fibers, coatings, gels and filters.
Claim 22
Dependent Claim
30 words
The conjugate of claims 9 or 11 wherein the monomer is a polyunsaturated oligomer having at least one reactive site for bonding with the antibody or intermediate "spacer arm" compound.
Claim 23
Dependent Claim
28 words
The conjugate of claim 16 wherein the nonderivatized compound is selected from the group consisting of olefinically unsaturated compounds, acetylenically unsaturated compounds, polyunsaturated compounds and multi-functional cross-linking compounds.
Claim 24
Dependent Claim
21 words
The conjugate of claims 9 or 11 wherein the monomer is one selected from the group consisting of: ##STR10## where ##STR11##
Claim 25
Independent Claim
79 words
A method of selectively binding a compound for removal from a solution or suspension containing the compound, comprising: providing a monomer/reactant conjugate; providing a solid surface having at least one polymerizable compound bonded thereto capable of copolymerizing with the monomer/reactant conjugate; contacting the compound to be removed with the monomer/reactant conjugate to bind the compound to the reactant and form a monomer/reactant--compound complex; and polymerizing the monomer in the presence of the polymerizable compound bonded to the solid surface.
Claim 26
Independent Claim
79 words
A method of selectively binding a compound for removal from a solution or suspension containing the compound, comprising: providing a monomer/reactant conjugate; providing a solid surface having at least one polymerizable compound bonded thereto capable of copolymerizing with the monomer/reactant conjugate; contacting the compound to be removed with the monomer/reactant conjugate to bind the compound to the reactant and form a monomer/reactant--compound complex; and polymerizing the monomer in the presence of the polymerizable compound bonded to the solid surface.
Claim 27
Dependent Claim
32 words
The method of claim 23 wherein the polymerizable compound is bonded to the solid surface by chemically modifying the surface to render it reactable with pendant groups attached to the polymerizable compound.
Claim 28
Dependent Claim
21 words
The method of claim 24 wherein the solid surface includes aromatic groups reactive with pendant groups attached to a vinyl monomer.
Claim 29
Dependent Claim
17 words
The method of claim 24, including creating initiation sites on the solid surface from free radical polymerization.
References Cited (Backward Citations)
US2853457A
Cited
US3969287A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-09-01 | 1990-03-03 | 1990-09-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-09-01 | 1994-03-03 | 1994-09-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-09-01 | 1998-03-03 | 1998-09-02 | $7700.00 | Window Closed / Lapsed |