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US4582622A
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Magnetic particulate for immobilization of biological protein and process of producing the same
Assignee: Fujirebio Inc
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Filing: 1984-10-12
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Granted: 1986-04-15
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Expires: 2004-10-12
Abstract
Abstract A magnetic particulate comprising gelatin, water-soluble polysaccharide, sodium polymetaphosphate and ferromagnetic substance, which is used as a carrier for immobilization of biological proteins such as antigens, antibodies or enzymes, and a process of producing the magnetic particulate.
Inventors
- 👤 Mikio Ikeda
- 👤 Shiro Sakamoto
- 👤 Kazumasa Suzuki
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 20:08
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54313; G01N33/54326; G01N33/5434; B; B03; B03C; B03C1/00; B03C1/005; B03C1/01; G01N2446/00; G01N2446/20; G01N2446/30; G03; G03C; G03C1/00; G03C1/005; G03C1/04; G03C1/047; G03C2001/0471; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/813
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
59 words
A magnetic particulate for immobilization of biological proteins which comprises 5% to 30% by weight of gelatin, 1% to 5% by weight of a water-soluble polysaccharide, 0.01% to 1% by weight of sodium polymetaphosphate having the formula (NaPO 3 ) n , 0.00001% to 2% by weignt of ferromagnetic substance and 62% to 94% by weight of a water.
Claim 2
Dependent Claim
59 words
A magnetic particulate for immobilization of biological proteins which comprises 5% to 30% by weight of gelatin, 1% to 5% by weight of a water-soluble polysaccharide, 0.01% to 1% by weight of sodium polymetaphosphate having the formula (NaPO 3 ) n , 0.00001% to 2% by weignt of ferromagnetic substance and 62% to 94% by weight of a water.
Claim 3
Dependent Claim
21 words
The magnetic particulate of claim 1, wherein said gelatin has an isoelectric point at a pH value between 8.4 and 9.1.
Claim 4
Dependent Claim
25 words
The magnetic particulate of claim 1, wherein said water-soluble polysaccharide is selected from the group consisting of gum arabic, carboxymethyl cellulose, sodium alginate and agar.
Claim 5
Dependent Claim
26 words
The magnetic particulate of claim 1, wherein said sodium polymetaphosphate has the formula (NaPO 3 ) n wherein n is an integer of 3 to 6.
Claim 6
Dependent Claim
47 words
The magnetic particulate of claim 1, wherein said ferromagnetic substance is a ferrite having the formula MFe 2 O 4 wherein M is a divalent metal selected from the group consisting of Fe ++ , Mn ++ , Cu ++ , Ni ++ and Co ++ .
Claim 7
Dependent Claim
60 words
A process for producing a magnetic particulate for immobilization of biological proteins which comprises the steps of: preparing an aqueous colloidal solution containing gelatin, a water-soluble polysaccharide, sodium polymetaphosphate and ferromagnetic substance, adjusting the pH of said colloidal solution to between 2.5 and 6.0 with an acid, and forming a water-insoluble particulate by adding an aldehyde to said colloidal solution.
Claim 8
Dependent Claim
60 words
A process for producing a magnetic particulate for immobilization of biological proteins which comprises the steps of: preparing an aqueous colloidal solution containing gelatin, a water-soluble polysaccharide, sodium polymetaphosphate and ferromagnetic substance, adjusting the pH of said colloidal solution to between 2.5 and 6.0 with an acid, and forming a water-insoluble particulate by adding an aldehyde to said colloidal solution.
Claim 9
Dependent Claim
20 words
The process of claim 6, wherein said gelatin has an isoelectric point at a pH value between 8.4 and 9.1.
Claim 10
Dependent Claim
21 words
The process of claim 6, wherein the concentration of gelatin is between 0.01% and 2% by weight of said colloidal solution.
Claim 11
Dependent Claim
22 words
The process of claim 6, wherein the concentration of water-soluble polysaccharide is between 0.01% and 2% by weight of said colloidal solution.
Claim 12
Dependent Claim
24 words
The process of claim 9, wherein said water-soluble polysaccharide is selected from the group consisting of gum arabic, carboxymethyl cellulose, sodium alginate and agar.
Claim 13
Dependent Claim
22 words
The process of claim 6, wherein the concentration of sodium polymetaphosphate is between 0.001% and 2% by weight of said colloidal solution.
Claim 14
Dependent Claim
27 words
The process of claim 11, wherein said sodium polymetaphosphate has the formula (NaPO 3 ) n , wherein n is an integer of from 3 to 6.
Claim 15
Dependent Claim
24 words
The process of claim 6, wherein said ferromagnetic substance is present in an amount between 0.01% and 5% by weight of said colloidal solution.
Claim 16
Dependent Claim
47 words
The process of claim 13, wherein said ferromagnetic substance is a ferrite having the formula MFe 2 O 4 , wherein M is a divalent metal selected from the group consisting of Fe ++ , Mn ++ , Cu ++ , Ni ++ and Co ++ .
Claim 17
Dependent Claim
18 words
The process of claim 6, wherein said aldehyde is selected from the group consisting of glutaraldehyde and formaldehyde.
References Cited (Backward Citations)
US4416813A
Cited
US4519931A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-04-14 | 1989-10-14 | 1990-04-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-04-14 | 1993-10-14 | 1994-04-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-04-14 | 1997-10-14 | 1998-04-15 | $7700.00 | Window Closed / Lapsed |