US4416813A
Expired / Lapsed
US
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Artificial carrier for immobilization of biological proteins
Assignee: Fujirebio Inc
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Filing: 1982-03-18
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Granted: 1983-11-22
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Expires: 2002-03-18
Abstract
Abstract A novel artificial carrier is disclosed which is prepared from gelatin, a water-soluble polysaccharide, a sodium metaphosphate and an aldehyde. The carrier can be used to immobilize antibodies, antigens or enzymes. Immobilized antigens and antibodies can be used as a diagnostic reagent in assays involving antigen-antibody reactions.
Inventors
- 👤 Mikio Ikeda
- 👤 Takayuki Tomizawa
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 09:23
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/02; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/544; G01N33/545; G03; G03C; G03C1/00; G03C1/005; G03C1/04; G03C1/047; G03C2001/0471; Y; Y10; Y10S; Y10S435/00; Y10S435/967; Y10S524/00; Y10S524/90; Y10S530/00; Y10S530/806; Y10S530/81; Y10S530/812; Y10S530/813; Y10S530/814
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
91 words
A process for producing an artificial carrier for immobilization of biological proteins which comprises: forming a solution of gelatin, a water soluble polysaccharide and sodium metaphosphate having the formula (NaPO 3 ) n , wherein n is a whole number from 3 to 6 at a temperature above the gelation temperature of said gelatin; mixing with agitation an acid with said solution to adjust the pH of said solution to between about 2.5 and 6 whereby particles are formed and treating said particles with an aldehyde to thereby insolubilize said particles.
Claim 2
Dependent Claim
91 words
A process for producing an artificial carrier for immobilization of biological proteins which comprises: forming a solution of gelatin, a water soluble polysaccharide and sodium metaphosphate having the formula (NaPO 3 ) n , wherein n is a whole number from 3 to 6 at a temperature above the gelation temperature of said gelatin; mixing with agitation an acid with said solution to adjust the pH of said solution to between about 2.5 and 6 whereby particles are formed and treating said particles with an aldehyde to thereby insolubilize said particles.
Claim 3
Dependent Claim
21 words
The process of claim 1, wherein the concentration of gelatin is between about 0.01 and 2% by weight of said solution.
Claim 4
Dependent Claim
22 words
The process of claim 1, wherein said gelatin has an isoelectric point at a pH value of between about 8 and 9.
Claim 5
Dependent Claim
21 words
The process of claim 1, wherein said water-soluble polysaccharide is present in an amount between about 0.01 and 2% by weight.
Claim 6
Dependent Claim
24 words
The process of claim 4, wherein said water-soluble polysaccharide is selected from the group consisting of arabic gum, carboxymethyl cellulose, sodium alginate, and agar.
Claim 7
Dependent Claim
21 words
The process of claim 1, wherein said sodium metaphosphate is selected from the group consisting of sodium trimetaphosphate and sodium hexametaphosphate.
Claim 8
Dependent Claim
16 words
The process of claim 1, wherein said reaction temperature is between about 25° and 50° C.
Claim 9
Dependent Claim
15 words
The process of claim 7, wherein said temperature is between about 35° and 50° C.
Claim 10
Dependent Claim
19 words
The process of claim 1, further comprising adjusting the pH of said solution to between about 4.0 and 5.5.
Claim 11
Dependent Claim
16 words
The process of claim 1 or 9, further comprising adjusting the pH with a weak acid.
Claim 12
Dependent Claim
14 words
The process of claim 1, further comprising adding a water-miscible solvent to said solution.
Claim 13
Dependent Claim
20 words
The process of claim 11, wherein said water-miscible solvent is selected from the group consisting of lower alcohols and acetone.
Claim 14
Dependent Claim
31 words
The process of claim 1, further comprising dissolving said water-soluble polysaccharide in a first solution and adding said first solution to a second solution comprising said gelatin and said sodium metaphosphate.
Claim 15
Dependent Claim
22 words
The process of claim 1, wherein said aldehyde is selected from the group consisting of glutaraldehyde, formaldehyde, glyoxal, crotonaldehyde, acrolein and acetoaldehyde.
Claim 16
Dependent Claim
10 words
The process of claim 14, wherein said aldehyde is glutaraldehyde.
Claim 17
Dependent Claim
25 words
The process of claim 14 or 15, wherein the amount of said aldehyde is between about 0.1 and 200% by dry weight of said gelatin.
Claim 18
Dependent Claim
31 words
The process of claim 1 further comprising cooling said solution to a temperature below 10° C. after the pH of the solution has been adjusted to between about 2.5 and 6.
Claim 19
Dependent Claim
14 words
The process of claim 1, further comprising adding an anionic surfactant to said solution.
Claim 20
Dependent Claim
30 words
The process of claim 18, wherein said anionic surfactant is selected from the group consisting of alkylsulfosuccinic acids, alkylsulfomaleic acids, alkylsulfuric acid esters, and polyoxyethylene alkyl ether sulfuric acid esters.
Claim 21
Dependent Claim
14 words
The process of claim 1, further comprising adding a nonionic surfactant to said solution.
Claim 22
Dependent Claim
33 words
The process of claim 20, wherein said nonionic surfactant is selected from the group consisting of polyoxyethylene fatty acid esters, polyoxyethylene alkyl ethers, polyoxyethylene alkyl phenyl esters, and polyethylene glycol fatty acid esters.
Claim 23
Dependent Claim
14 words
The process of claim 1, further comprising adding a coloring agent to said solution.
Claim 24
Dependent Claim
9 words
The product produced by the process of claim 1.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1986-11-21 | 1987-05-23 | 1987-11-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1990-11-21 | 1991-05-23 | 1991-11-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1994-11-21 | 1995-05-23 | 1995-11-22 | $7700.00 | Window Closed / Lapsed |