Patent US3525673A successfully refreshed from Google Patents.
Patent US4581847A successfully refreshed from Google Patents.
US4581847A
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Tryptophan overproducer mutants of cereal crops
Assignee: DeKalb Genetics Corp
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Filing: 1984-09-04
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Granted: 1986-04-15
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Expires: 2004-09-04
Abstract
Abstract This invention is directed to the production of novel plant genotypes or lines which produce increased free tryptophan levels and are resistant to inhibition by analogs which normally inhibit the activity of the enzyme anthranilate synthase before alteration. This invention further relates to genes encoding such enzymes, and to processes for utilizing these novel genes and enzymes. Further products of the invention include plants, plant tissues and seeds which exhibit resistance to such analogs and overproduce tryptophan resulting from expression of genes encoding analog resistant anthranilate synthase enzyme.
Inventors
- 👤 Kenneth A. Hibberd
- 👤 Paul C. Anderson
- 👤 Melanie Barker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 19:19
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/88; A; A01; A01H; A01H5/00; A01H5/10; A01H6/00; A01H6/46; A01H6/4684; C12N15/00; C12N15/01; C12N15/09; C12N15/63; C12N15/79; C12N15/82; C12N15/8241; C12N15/8242; C12N15/8243; C12N15/8251; C12N15/8254; C12N5/00; C12N5/04
Claims Analysis
Total Claims: 58
Claim 1
Independent Claim
48 words
A maize seed having an endogenious free tryptophan content of at least about one-tenth milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 2
Dependent Claim
48 words
A maize seed having an endogenious free tryptophan content of at least about one-tenth milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 3
Dependent Claim
52 words
The maize seed according to claim 1, having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 4
Dependent Claim
62 words
A tryptophan overproducing maize seed capable of germinating into a plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 5
Dependent Claim
62 words
A tryptophan overproducing maize seed capable of germinating into a plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 6
Dependent Claim
103 words
A tryptophan overproducing maize seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptopha,n, as measured after six days incubation.
Claim 7
Dependent Claim
103 words
A tryptophan overproducing maize seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptopha,n, as measured after six days incubation.
Claim 8
Dependent Claim
15 words
A maize seed deposited with In Vitro International, Inc. and assigned IVI accession No. 10010.
Claim 9
Dependent Claim
15 words
A maize seed deposited with In Vitro International, Inc. and assigned IVI accession No. 10010.
Claim 10
Dependent Claim
11 words
A maize seed resulting from crossing C-28 with another maize line.
Claim 11
Dependent Claim
11 words
A maize seed resulting from crossing C-28 with another maize line.
Claim 12
Dependent Claim
49 words
A hybrid maize seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 13
Dependent Claim
49 words
A hybrid maize seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 14
Dependent Claim
53 words
The hybrid mazie seed according to claim 7, having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight and capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 15
Dependent Claim
63 words
A tryptophan overproducing hybrid maize seed capable of germinating into a plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 16
Dependent Claim
63 words
A tryptophan overproducing hybrid maize seed capable of germinating into a plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 17
Dependent Claim
104 words
A tryptophan overproducing hybrid maize seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation.
Claim 18
Dependent Claim
104 words
A tryptophan overproducing hybrid maize seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation.
Claim 19
Dependent Claim
55 words
A maize plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 20
Dependent Claim
55 words
A maize plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 21
Dependent Claim
63 words
The maize plant according to claim 11, wherein the plant is capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 22
Dependent Claim
56 words
A tryptophan overproducing maize plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 23
Dependent Claim
56 words
A tryptophan overproducing maize plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 24
Dependent Claim
107 words
A tryptophan overproducing maize plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation.
Claim 25
Dependent Claim
107 words
A tryptophan overproducing maize plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation.
Claim 26
Dependent Claim
18 words
A maize plant derived from seeds deposited with In Vitro International, Inc. and assigned IVI accession No. 10010.
Claim 27
Dependent Claim
18 words
A maize plant derived from seeds deposited with In Vitro International, Inc. and assigned IVI accession No. 10010.
Claim 28
Dependent Claim
20 words
A maize plant having an average endogenous free tryptophan content of at least about 0.04 milligrams per gram fresh weight.
Claim 29
Dependent Claim
20 words
A maize plant having an average endogenous free tryptophan content of at least about 0.04 milligrams per gram fresh weight.
Claim 30
Dependent Claim
24 words
The maize plant according to claim 16, having an average endogenous free tryptophan content of at least about one-tenth milligram per gram fresh weight.
Claim 31
Dependent Claim
56 words
A hybrid maize plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 32
Dependent Claim
56 words
A hybrid maize plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 33
Dependent Claim
64 words
The hybrid maize plant according to claim 18, wherein the plant is capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 34
Dependent Claim
57 words
A tryptophan overproducing hybrid maize plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 35
Dependent Claim
57 words
A tryptophan overproducing hybrid maize plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 36
Dependent Claim
108 words
A tryptophan overproducing hybrid maize plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation.
Claim 37
Dependent Claim
108 words
A tryptophan overproducing hybrid maize plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation.
Claim 38
Dependent Claim
61 words
A maize tissue culture capable of generating a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 39
Dependent Claim
61 words
A maize tissue culture capable of generating a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight.
Claim 40
Dependent Claim
64 words
The maize tissue culture according to claim 22, wherein the plant is capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, wherein the seed is capable of germinating into a plant capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight.
Claim 41
Dependent Claim
62 words
A tryptophan overproducing maize tissue culture capable of generating a plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 42
Dependent Claim
62 words
A tryptophan overproducing maize tissue culture capable of generating a plant capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, wherein the plant is capable of transmitting the gene encoding the anthranilate synthase to its progeny.
Claim 43
Dependent Claim
112 words
A tryptophan overproducing maize tissue culture capable of generating a plant capable of producing seed having an embryo capable of producing in the presence 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation.
Claim 44
Dependent Claim
112 words
A tryptophan overproducing maize tissue culture capable of generating a plant capable of producing seed having an embryo capable of producing in the presence 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, wherein the seed is capable of germinating into a plant capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about 60 percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation.
Claim 45
Dependent Claim
19 words
A maize tissue culture derived from seeds deposited with In Vitro International, Inc. and assigned IVI accession No. 10010.
Claim 46
Dependent Claim
19 words
A maize tissue culture derived from seeds deposited with In Vitro International, Inc. and assigned IVI accession No. 10010.
Claim 47
Dependent Claim
21 words
A maize tissue culture having an average endogenous free tryptophan content of at least about 0.78 milligrams per gram dry weight.
Claim 48
Dependent Claim
21 words
A maize tissue culture having an average endogenous free tryptophan content of at least about 0.78 milligrams per gram dry weight.
Claim 49
Independent Claim
136 words
A method for producing a tryptophan overproducing maize plant, comprising the steps of: (a) isolating an appropriate donor tissue from a maize plant and growing said tissue in an initiation/maintenance medium to initiate and maintain the growth of plant tissue cultures; (b) growing said plant tissue cultures in the presence of a tryptophan analog at a level which inhibits from about 60% to about 80% of growth during the first four weeks; (c) repeated subculturing the plant tissue cultures of step (b) in the presence of the tryptophan analog; (d) identifying a stable analog resistant plant cell line from step (c) which grows at a rate comparable to that of a plant tissue culture grown in the absence of a tryptophan analog; and (e) regenerating a maize plant from said stable analog resistant plant cell line.
Claim 50
Independent Claim
136 words
A method for producing a tryptophan overproducing maize plant, comprising the steps of: (a) isolating an appropriate donor tissue from a maize plant and growing said tissue in an initiation/maintenance medium to initiate and maintain the growth of plant tissue cultures; (b) growing said plant tissue cultures in the presence of a tryptophan analog at a level which inhibits from about 60% to about 80% of growth during the first four weeks; (c) repeated subculturing the plant tissue cultures of step (b) in the presence of the tryptophan analog; (d) identifying a stable analog resistant plant cell line from step (c) which grows at a rate comparable to that of a plant tissue culture grown in the absence of a tryptophan analog; and (e) regenerating a maize plant from said stable analog resistant plant cell line.
Claim 51
Independent Claim
38 words
A method for producing hybrid maize seed, comprising crossing a first maize line capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, and another maize line.
Claim 52
Independent Claim
38 words
A method for producing hybrid maize seed, comprising crossing a first maize line capable of producing seed having an endogenous free tryptophan content of at least about one-tenth milligram per gram dry seed weight, and another maize line.
Claim 53
Independent Claim
38 words
A method for producing hybrid maize seed, comprising crossing a first maize line capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, and another maize line.
Claim 54
Independent Claim
38 words
A method for producing hybrid maize seed, comprising crossing a first maize line capable of producing seed having an endogenous free tryptophan content of at least about one milligram per gram dry seed weight, and another maize line.
Claim 55
Independent Claim
52 words
A method for producing hybrid maize seed, comprising crossing a first maize line capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, and another maize line.
Claim 56
Independent Claim
52 words
A method for producing hybrid maize seed, comprising crossing a first maize line capable of expressing a gene coding for an anthranilate synthase which retains from about 60 to about 80 percent of its activity in the presence of 5-methyltryptophan at a concentration of about 10 -5 M, and another maize line.
Claim 57
Independent Claim
62 words
A method for producing hybrid maize seed, comprising crossing a first maize line capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, and another maize line.
Claim 58
Independent Claim
62 words
A method for producing hybrid maize seed, comprising crossing a first maize line capable of producing seed having an embryo capable of producing in the presence of 5-methyltryptophan at a concentration of 10 -4 M roots of at least about percent of the length of roots produced in the absence of 5-methyltryptophan, as measured after six days incubation, and another maize line.
References Cited (Backward Citations)
US4443971A
Cited
USPTO Annuity & Maintenance Windows
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| 3.5 Years (Year 4) | 1989-04-14 | 1989-10-14 | 1990-04-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-04-14 | 1993-10-14 | 1994-04-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-04-14 | 1997-10-14 | 1998-04-15 | $7700.00 | Window Closed / Lapsed |