US4581331A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for the rapid detection of virus and viral antigens
Assignee: Michigan State University
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Filing: 1983-09-29
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Granted: 1986-04-08
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Expires: 2003-09-29
Abstract
Abstract A method for the rapid detection of virus and viral antigens associated with somatic cells in biological test samples is disclosed. The method comprises lysing the cells with a lytic agent to reveal virus or viral antigens for participation in a virus detection system. The lytic agent is chosen for its biological neutrality in the detection system, particularly its inability to produce a cytopathic effect in tissue culture.
Inventors
- 👤 James C. Richards
- 👤 Dennis L. Murray
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 22:10
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/569; G01N33/56983; C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/70; Y; Y10; Y10S; Y10S435/00; Y10S435/961; Y10S436/00; Y10S436/825
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
130 words
A method for the rapid detection and determination of virus or viral antigens suspected of being contained in somatic cells in a test sample, comprising: (i) forming a reaction mixture by contacting the test sample with a solution having contained therein a sapogenin glycoside capable of lysing the somatic cells to release virus or viral antigens contained therein or thereon: (ii) separating the reaction mixture into a first fraction containing virus and viral antigens and the sapogenin glycoside and a second fraction substantially free of virus or viral antigens: (iii) contacting an aliquot of the first fraction with a virus detection system capable of exhibiting an effect attributable to the presence of the virus or viral antigens in the first fraction, but which is not attributable to the sapogenin glycoside.
Claim 2
Independent Claim
130 words
A method for the rapid detection and determination of virus or viral antigens suspected of being contained in somatic cells in a test sample, comprising: (i) forming a reaction mixture by contacting the test sample with a solution having contained therein a sapogenin glycoside capable of lysing the somatic cells to release virus or viral antigens contained therein or thereon: (ii) separating the reaction mixture into a first fraction containing virus and viral antigens and the sapogenin glycoside and a second fraction substantially free of virus or viral antigens: (iii) contacting an aliquot of the first fraction with a virus detection system capable of exhibiting an effect attributable to the presence of the virus or viral antigens in the first fraction, but which is not attributable to the sapogenin glycoside.
Claim 3
Dependent Claim
12 words
The method of claim 1 wherein the sapogenin glycoside is detoxified saponin.
Claim 4
Dependent Claim
11 words
The method of claim 1 wherein the separating step comprises centrifugation.
Claim 5
Dependent Claim
14 words
The method of claim 3 wherein the centrifugation is carried out at about 3000×g.
Claim 6
Dependent Claim
18 words
The method of claim 1 wherein the detection system comprises tissue culture susceptible to infection by the virus.
Claim 7
Dependent Claim
11 words
The method of claim 1 wherein the detection system comprises immunoassay.
Claim 8
Dependent Claim
13 words
The method of claim 1 wherein the detection system comprises nucleic acid hybridization.
Claim 9
Dependent Claim
18 words
The method of claim 1 wherein the detection system comprises live animals susceptible to infection by the virus.
References Cited (Backward Citations)
US3935066A
Cited
SE416959B
Cited
US3928139A
Cited
US3883425A
Cited
US3875012A
Cited
US3932222A
Cited
US4131512A
Cited
US4212948A
Cited
US4358535A
Cited
US4358535B1
Cited
US4497900A
Cited
EP0097633A1
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-04-07 | 1989-10-07 | 1990-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-04-07 | 1993-10-07 | 1994-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-04-07 | 1997-10-07 | 1998-04-08 | $7700.00 | Window Closed / Lapsed |