US3883425A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Detoxification of saponins
Assignee: WADLEY TECHNOLOGIES Inc A CORP OF TX
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Filing: 1973-12-10
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Granted: 1975-05-13
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Expires: 1993-12-10
Abstract
Abstract Saponin material extracted from plants is purified and made nontoxic to microbial organisms by removing constituents from an aqueous solution of the saponin extract which have an apparent molecular weight of less than about 600 in the aqueous solution. In a preferred embodiment, an aqueous solution of saponin is prepared and then passed through a filter membrane having average pore sizes no smaller than about 11 angstroms in diameter and no larger than about 24 angstroms in diameter while adding amounts of pure aqueous solvent to the solution approximately equal in volume to the liquid passing through the membrane. The toxic material will be in the liquid passed through the membrane.
Inventors
- 👤 Gordon L Dorn
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 22:45
CPC Classifications
B; B01; B01D; B01D61/00; B01D61/14; B01D61/145
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
40 words
IN A METHOD OF BOTAINING PURIFIED SAPONIN FROM A PLANT SOURCE WHEREIN A COMPONENT RICH IN SAPONIN AND WHICH WILL HEMOLYZE RED BLOOD CELLS IS EXTRACTED FROM SAID PLANT SOURCE, THE IMPROVEMENT COMPRISING: REMOVING ANTIMICROBIAL TOXIC CONSTITUENTS FROM SAID SAPONIN
Claim 2
Dependent Claim
40 words
IN A METHOD OF BOTAINING PURIFIED SAPONIN FROM A PLANT SOURCE WHEREIN A COMPONENT RICH IN SAPONIN AND WHICH WILL HEMOLYZE RED BLOOD CELLS IS EXTRACTED FROM SAID PLANT SOURCE, THE IMPROVEMENT COMPRISING: REMOVING ANTIMICROBIAL TOXIC CONSTITUENTS FROM SAID SAPONIN
Claim 3
Dependent Claim
43 words
The method of claim 1 wherein said antimicrobial toxic constituents are removed from said saponin extract by providing an aqueous solution of said saponin extract and thereafter separating toxic materials therefrom which have an apparent molecular weight in said solution below about 600.
Claim 4
Dependent Claim
19 words
The method of claim 2 wherein said antimicrobial toxic constituents are removed from said aqueous solution by gel filtration.
Claim 5
Dependent Claim
18 words
The method of claim 2 wherein said antimicrobial toxic constituents are removed from said aqueous solution by ultrafiltration.
Claim 6
Dependent Claim
10 words
The purified saponin extract produced by the process of claim
Claim 7
Independent Claim
89 words
A method of removing antimicrobial toxins from saponin extract of a plant which will hemolyze red blood cells comprising: a. providing an aqueous solution of said saponin extract; b. passing said aqueous solution through a filter membrane having a mean pore size no smaller than about 11 angstroms and no larger than about 24 angstroms to thereby cause said toxins to pass therethrough, but yet exclude said saponin molecules; and c. separating the excluded saponin molecules from the remaining portions of said solution which pass through said filter membrane.
Claim 8
Independent Claim
89 words
A method of removing antimicrobial toxins from saponin extract of a plant which will hemolyze red blood cells comprising: a. providing an aqueous solution of said saponin extract; b. passing said aqueous solution through a filter membrane having a mean pore size no smaller than about 11 angstroms and no larger than about 24 angstroms to thereby cause said toxins to pass therethrough, but yet exclude said saponin molecules; and c. separating the excluded saponin molecules from the remaining portions of said solution which pass through said filter membrane.
Claim 9
Dependent Claim
50 words
The method of claim 6 carried out by an ultrafiltration technique wherein volumes of aqueous solvent used to form said aqueous solution are added to said aqueous solution in volumetric increments equal to the volume of the solution passing through said filter membrane as said solution passes through said membrane.
Claim 10
Dependent Claim
21 words
The method of claim 6 wherein said aqueous solution comprises from about 5 to about 50% (W/V) of said saponin extract.
Claim 11
Dependent Claim
12 words
The method of claim 8 wherein said aqueous solvent is distilled water.
Claim 12
Dependent Claim
13 words
The method of claim 8 wherein said aqueous solvent is normal saline solution.
Claim 13
Dependent Claim
11 words
The purified saponin extract produced by the process of claim 6.
Claim 14
Independent Claim
46 words
A method of removing antimicrobial toxins from saponin extract of a plant which will hemolyze red blood cells comprising: a. providing an aqueous solution of saiD saponin extract; and b. removing toxic materials therefrom which have an apparent molecular weight of said solution below about 600.
Claim 15
Independent Claim
46 words
A method of removing antimicrobial toxins from saponin extract of a plant which will hemolyze red blood cells comprising: a. providing an aqueous solution of saiD saponin extract; and b. removing toxic materials therefrom which have an apparent molecular weight of said solution below about 600.
Claim 16
Dependent Claim
27 words
The method of claim 12 wherein said toxic materials have an apparent molecular weight in aqueous solution in the range of from about 140 to about 600.
Claim 17
Dependent Claim
32 words
The method of claim 12 further comprising using the product of claim 12 to hemolyze red blood cells in a blood sample, and thereafter subjecting said sample to a bacteria culturing process.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-05-12 | 1978-11-11 | 1979-05-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-05-12 | 1982-11-11 | 1983-05-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-05-12 | 1986-11-11 | 1987-05-13 | $7700.00 | Window Closed / Lapsed |