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US4575485A
Expired / Lapsed
US
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Ultrasonic enhanced immuno-reactions
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1983-08-29
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Granted: 1986-03-11
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Expires: 2003-08-29
Abstract
Abstract Rates of binding between members of a specific binding pair, e.g., ligand-receptor, are greatly enhanced by short-term ultrasonication of an aqueous medium containing the specific binding pair. The enhanced rates find particular use in specific binding protein assays.
Inventors
- 👤 N. Chung Sizto
- 👤 Cindy Gallup
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 08:20
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/5306; Y; Y10; Y10S; Y10S435/00; Y10S435/962; Y10S435/966; Y10S435/97; Y10S436/00; Y10S436/824
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
46 words
In a method for measuring the binding of members of a specific binding pair in an aqueous medium, the improvement which comprises ultrasonicating the medium containing the members of the specific binding pair for a sufficient time to enhance the rate of binding of said members.
Claim 2
Dependent Claim
46 words
In a method for measuring the binding of members of a specific binding pair in an aqueous medium, the improvement which comprises ultrasonicating the medium containing the members of the specific binding pair for a sufficient time to enhance the rate of binding of said members.
Claim 3
Dependent Claim
17 words
The method of claim 1 wherein the members of the specific binding pair are ligand and receptor.
Claim 4
Dependent Claim
20 words
The method of claim 1 wherein the medium is ultrasonicated at a frequency of about 5 to 10 3 kHz
Claim 5
Dependent Claim
20 words
The method of claim 1 wherein the medium is ultrasonicated for a period of about 30 seconds to 2 hours.
Claim 6
Dependent Claim
19 words
The method of claim 1 wherein the medium is ultrasonicated at a temperature of about 15° to 40° C.
Claim 7
Dependent Claim
34 words
The method of claim 1 wherein the medium further contains a signal producing system capable of producing a detectable signal which is dependent on the binding of the members of the specific binding pair.
Claim 8
Dependent Claim
20 words
The method of claim 1 wherein one of the members of the specific binding pair is bound to a support.
Claim 9
Dependent Claim
92 words
In an assay method for determining the presence in a sample of a first member of a specific binding pair wherein the sample is combined in an aqueous medium with at least a second member of the specific binding pair and components of a signal producing system capable of producing a detectable signal in relation to the amount of said first member, the improvement which comprises ultrasonicating the mixture for a time and at an intensity sufficient to enhance the rate of the binding of the members of the specific binding pair.
Claim 10
Dependent Claim
92 words
In an assay method for determining the presence in a sample of a first member of a specific binding pair wherein the sample is combined in an aqueous medium with at least a second member of the specific binding pair and components of a signal producing system capable of producing a detectable signal in relation to the amount of said first member, the improvement which comprises ultrasonicating the mixture for a time and at an intensity sufficient to enhance the rate of the binding of the members of the specific binding pair.
Claim 11
Dependent Claim
17 words
The method of claim 8 wherein the members of the specific binding pair are ligand and receptor.
Claim 12
Dependent Claim
11 words
The method of claim 9 wherein the ligand is a hapten.
Claim 13
Dependent Claim
11 words
The method of claim 9 wherein the ligand is an antigen.
Claim 14
Dependent Claim
11 words
The method of claim 9 wherein the receptor is an antibody.
Claim 15
Dependent Claim
20 words
The method of claim 8 wherein the medium is ultrasonicated at a frequency of about 5 to 10 3 kHz
Claim 16
Dependent Claim
20 words
The method of claim 8 wherein the medium is ultrasonicated for a period of about 30 seconds to 2 hours.
Claim 17
Dependent Claim
19 words
The method of claim 8 wherein the medium is ultrasonicated at a temperature of about 15° to 40° C.
Claim 18
Dependent Claim
29 words
The method of claim 8 wherein one of the components of the signal producing system is an enzyme conjugated to one of the members of the specific binding pair.
Claim 19
Dependent Claim
29 words
The method of claim 8 wherein one of the components of the signal producing system is a fluorescer conjugated to one of the members of the specific binding pair.
Claim 20
Dependent Claim
20 words
The method of claim 8 wherein one of the members of the specific binding pair is bound to a support.
Claim 21
Dependent Claim
12 words
The method of claim 18 wherein the support is a solid strip.
Claim 22
Dependent Claim
22 words
The method of claim 8 wherein at least one of the components of the signal producing system is bound to a dip-stick.
Claim 23
Dependent Claim
36 words
The method of claim 8 wherein components of the signal producing system comprise an enzyme bound to said dip-stick and a second enzyme where the substrate of one enzyme is the product of the other enzyme.
Claim 24
Dependent Claim
19 words
The method of claim 8 wherein the member of a specific binding pair in the sample is a protein.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-03-10 | 1989-09-09 | 1990-03-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-03-10 | 1993-09-09 | 1994-03-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-03-10 | 1997-09-09 | 1998-03-11 | $7700.00 | Window Closed / Lapsed |