Patent US4567150A successfully refreshed from Google Patents.
US4567150A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for determining transferrin and composition therefor
Assignee: Sclavo SpA
•
Filing: 1984-01-18
•
Granted: 1986-01-28
•
Expires: 2004-01-18
Abstract
Abstract A method for determining the iron-binding capability of transferrin, consisting in adding to the sample being tested a reagent composed of a solution of a ferric salt adsorbed onto a suspension of magnesium carbonate, centrifuging the mixture so obtained and measuring the iron blocked by transferrin in the supernatant. The method is preferably carried out by utilizing a particular composition consisting of a single reagent composed of a suspension of a solution of a ferric salt absorbed on magnesium carbonate, possibly a medium capable of maintaining the pH between 8 and 9, if desired, a colorimetric reagent for the determination of iron.
Inventors
- 👤 Alessandro Tabacco
- 👤 Paolo Tarli
- 👤 Paolo Neri
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 20:50
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/90; Y; Y10; Y10S; Y10S436/00; Y10S436/91
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
70 words
A method for determining transferrin comprising the steps of adding to the sample being tested a reagent comprising a solution of a ferric salt adsorbed on an aqueous suspension buffered to a pH of between 8 and 9 of a solid substrate selected from the group consisting of basic magnesium carbonate and calcium carbonate, centrifuging the mixture thus obtained, and measuring the iron bonded by transferrin in the supernatant liquor.
Claim 2
Independent Claim
70 words
A method for determining transferrin comprising the steps of adding to the sample being tested a reagent comprising a solution of a ferric salt adsorbed on an aqueous suspension buffered to a pH of between 8 and 9 of a solid substrate selected from the group consisting of basic magnesium carbonate and calcium carbonate, centrifuging the mixture thus obtained, and measuring the iron bonded by transferrin in the supernatant liquor.
Claim 3
Dependent Claim
17 words
Method for determining transferrin according to claim 1, characterized in that the solid substrate is calcium carbonate.
Claim 4
Dependent Claim
29 words
Method for determining transferrin according to claim 2, characterized in that the buffer is a member selected from the group consisting of phosphoric acid, phosphates, barbituric acid and barbiturates.
Claim 5
Dependent Claim
44 words
A composition adapted to determining transferring consisting of a solution of a ferric salt combined with a solid adsorbent phase selected from the group consisting of magnesium carbonate and calcium carbonate and a buffer means capable of maintaining the pH between 8 and 9.
Claim 6
Dependent Claim
44 words
A composition adapted to determining transferring consisting of a solution of a ferric salt combined with a solid adsorbent phase selected from the group consisting of magnesium carbonate and calcium carbonate and a buffer means capable of maintaining the pH between 8 and 9.
Claim 7
Dependent Claim
31 words
Composition adapted to determining transferrin according to claim 4, characterized in that the buffer means is a member selected from the group consisting of phosphoric acid, phosphates, barbituric acid and barbiturates.
Claim 8
Dependent Claim
51 words
A composition adapted to determining transferrin consisting of a solution of ferric salt combined with a solid adsorbent phase selected from the group consisting of magnesium carbonate and calcium carbonate, a buffer means capable of maintaining the pH between 8 and 9, and a colorimetric reagent for the determination of iron.
Claim 9
Dependent Claim
51 words
A composition adapted to determining transferrin consisting of a solution of ferric salt combined with a solid adsorbent phase selected from the group consisting of magnesium carbonate and calcium carbonate, a buffer means capable of maintaining the pH between 8 and 9, and a colorimetric reagent for the determination of iron.
Claim 10
Dependent Claim
23 words
A composition as defined in claim 6 which includes from 0.5-10 g/dl of basic magnesium carbonate and from 1-100 mg/dl of ferric ion.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-01-27 | 1989-07-29 | 1990-01-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-01-27 | 1993-07-29 | 1994-01-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-01-27 | 1997-07-29 | 1998-01-28 | $7700.00 | Window Closed / Lapsed |