US3773467A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Microassay diagnostic test method
Assignee: Wilson Pharmaceutical and Chemical Corp
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Filing: 1971-11-17
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Granted: 1973-11-20
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Expires: 1991-11-17
Abstract
Abstract The method of determining the total iron binding capacity (TIBC) of human blood involves removing the iron content of an aliquot of blood, adding a solution of radioactive ferric cations to the iron free aliquot, incubating the mixture at a pH in the range of 7 to 9 to effectuate coupling of ferric cations and protein, measuring the initial radioactivity of the mixture, contacting the mixture with anion exchange resin to produce a solution free of unassociated radioactive ferric cations and measuring residual radioactivity of said solution free of unassociated radioactive ferric cations.
Inventors
- 👤 S Yang
- 👤 S Egozi
- 👤 S Yanari
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 16:55
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/90
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
15 words
A method according to claim 1 wherein said liquid portion of human blood is serum.
Claim 2
Dependent Claim
15 words
A method according to claim 1 wherein said liquid portion of human blood is plasma.
Claim 3
Dependent Claim
15 words
A method according to claim 1 wherein said acidic iron isolating agent is citric acid.
Claim 4
Dependent Claim
32 words
A method of determining the natural iron content of human blood protein which comprises subtracting from the TIBC content determined in accordance with claim 1 the UIBC determined by a conventional method.
Claim 5
Independent Claim
189 words
A method of determining the total iron binding capacity of the liquid portion of human blood containing transferrin protein molecules which comprises adjusting the acidity of an aliquot of said liquid portion of human blood to a pH in the range between 4 and 5.5 with a buffer solution containing citrate ion-iron isolating agent whose ferriccitrate complex has an affinity for anion resin, contacting said pH adjusted liquid with a strong base anion exchange resin, separating the iron-free liquid from said resin, mixing with said iron-free liquid an aqueous buffer solution containing citrate ions, bicarbonate ions and radioactive ferric ions, to produce a mixture having a pH in the range of 7 to 9, incubating the mixture to effectuate coupling of radioactive ferric ions and transferrin, measuring the initial radioactivity of the mixture, contacting said mixture containing iron saturated transferrin and the excess iron present in the form of iron ferric citrate complex with anion exchange resin to produce a solution containing iron essentially only in the form of iron saturated transferrin, and measuring residual radioactivity of said contacted anion resin or said solution containing said iron saturated transferrin.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-11-19 | 1977-05-21 | 1977-11-20 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-11-19 | 1981-05-21 | 1981-11-20 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-11-19 | 1985-05-21 | 1985-11-20 | $7700.00 | Window Closed / Lapsed |