Patent US4564600A successfully refreshed from Google Patents.
US4564600A
Expired / Lapsed
US
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Allergens and antibodies from allergen extracts and pooled serum
Assignee: Individual
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Filing: 1982-10-26
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Granted: 1986-01-14
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Expires: 2002-10-26
Abstract
Abstract Allergenic factors are isolated from antigen extracts by preparing a column with pooled serum from patients with allergy to a given allergen system and passing over this column a pool of allergen extracts from different sources. Allergen specific immunoglobulin antibodies in purified form are obtained by forming an antigen-specific antibody complex from serum containing the antibodies and a material containing the antigen, recovering the complex and disassociating it, forming a second complex of the antigen with an excess of a second antibody so as to realize a combination of the second complex, free first specific antibody and free second specific antibody and then separating the second complex from the free first and second antibodies.
Inventors
- 👤 Majid Ali
- 👤 Madhava P. Ramanarayanan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:28
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/35; A61K39/395; A61K39/39575; Y; Y10; Y10S; Y10S424/00; Y10S424/805; Y10S424/81; Y10S436/00; Y10S436/824; Y10S530/00; Y10S530/862; Y10S530/868
Claims Analysis
Total Claims: 22
Claim 1
Independent Claim
42 words
A method of isolating allergenic components from allergen extracts and of obtaining purified antigen-specific antibodies comprising forming an antigen-specific antibody complex from pooled serum containing said antibodies and pooled material containing said antigen, and separating said complex from said serum and material.
Claim 2
Independent Claim
42 words
A method of isolating allergenic components from allergen extracts and of obtaining purified antigen-specific antibodies comprising forming an antigen-specific antibody complex from pooled serum containing said antibodies and pooled material containing said antigen, and separating said complex from said serum and material.
Claim 3
Independent Claim
99 words
A method of isolating allergenic components from allergen extracts and of obtaining purified antigen-specific antibodies comprising forming a first antigen-specific antibody complex from serum containing said antibodies and a material containing said antigen, separating said first complex from said serum and material, dissociating said first complex, forming a second complex by mixing the dissociated first complex with an excess of a second antibody specific for said antigen so as to obtain a mixture of said second complex of antigen-second antibody, free first antibody and free second antibody, and separating said second complex from said free first and second antibodies.
Claim 4
Independent Claim
99 words
A method of isolating allergenic components from allergen extracts and of obtaining purified antigen-specific antibodies comprising forming a first antigen-specific antibody complex from serum containing said antibodies and a material containing said antigen, separating said first complex from said serum and material, dissociating said first complex, forming a second complex by mixing the dissociated first complex with an excess of a second antibody specific for said antigen so as to obtain a mixture of said second complex of antigen-second antibody, free first antibody and free second antibody, and separating said second complex from said free first and second antibodies.
Claim 5
Dependent Claim
31 words
The method of claim 2 so as to obtain purified specific IgE and IgG comprising (a) pooling serum of a plurality of allergic individuals, (b) pooling a plurality of allergen extracts,
Claim 6
Dependent Claim
66 words
(c) mixing the said pooled serum and pooled extracts under conditions permitting the formation of a first antigen complex, (d) separating the first complex from the mixed pools, (e) dissociating the first complex, (f) mixing the dissociated first complex with excess antigen specific IgG or antigen specific IgE, (g) forming a second antigen immunoglobulin complex and (h) separating the second antigen complex from free specific immunoglobulin.
Claim 7
Dependent Claim
66 words
(c) mixing the said pooled serum and pooled extracts under conditions permitting the formation of a first antigen complex, (d) separating the first complex from the mixed pools, (e) dissociating the first complex, (f) mixing the dissociated first complex with excess antigen specific IgG or antigen specific IgE, (g) forming a second antigen immunoglobulin complex and (h) separating the second antigen complex from free specific immunoglobulin.
Claim 8
Dependent Claim
39 words
The method of claim 3 wherein the first antigen complex and second antigen complex separation steps comprise precipitation, and wherein the steps of dissociating the first antigen complex and forming the second antigen complex are effected by pH adjustment.
Claim 9
Dependent Claim
18 words
The method of claim 4 wherein the precipitation is effected by employing polyethylene glycol as a precipitating agent.
Claim 10
Dependent Claim
34 words
The method of claim 5 wherein the dissociation is effected by adjusting the pH to about 2.5 and the formation of the second antigen complex is effected by adjusting the pH to about 7.6.
Claim 11
Dependent Claim
32 words
The method of claim 6, wherein the ratio of added antigen specific IgG or IgE to IgE or IgG, respectively, of the dissociated first complex in step (f) is at least 5:1.
Claim 12
Dependent Claim
11 words
The method of claim 7 wherein the ratio is about 10:1.
Claim 13
Dependent Claim
37 words
The method of claim 8 wherein the pooled sera are dialyzed gamma-globulin fraction pooled sera and a quantity of pooled extract having a protein content about 1-5 mg is mixed with each 60 ml of pooled serum.
Claim 14
Dependent Claim
24 words
The method of claim 3 for obtaining purified specific IgE wherein the excess specific IgG or IgE in step (f) is antigen specific IgG.
Claim 15
Dependent Claim
24 words
The method of claim 3 for obtaining purified specific IgG wherein the excess specific IgG or IgE in step (f) is antigen specific IgE.
Claim 16
Dependent Claim
37 words
The product of the process of claim 2 comprising the free first and second antibodies which are specific IgG and specific IgE and in which said immunoglobulins are present in a ratio of less than about 200:1.
Claim 17
Dependent Claim
13 words
The product of claim 12 having a ratio of less than about 100:1.
Claim 18
Dependent Claim
14 words
The product of claim 13 in which the ratio is of IgE to IgG.
Claim 19
Dependent Claim
11 words
The product of claim 14 having a ratio of about 10:1.
Claim 20
Dependent Claim
38 words
The product of the process of claim 3 comprising free first and second immunoglobulins which are specific IgG and specific IgE and in which said IgG and IgE are present in a ratio of less than about 200:1.
Claim 21
Dependent Claim
14 words
The product of claim 16 in which the ratio is of IgE to IgG.
Claim 22
Dependent Claim
11 words
The product of claim 17 having a ratio of about 10:1.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1989-01-13 | 1989-07-15 | 1990-01-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1993-01-13 | 1993-07-15 | 1994-01-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1997-01-13 | 1997-07-15 | 1998-01-14 | $7700.00 | Window Closed / Lapsed |