US4550080A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process for the preparation of a plasminogen activator
Assignee: Asahi Kasei Corp
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Filing: 1984-06-05
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Granted: 1985-10-29
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Expires: 2004-06-05
Abstract
Abstract A process for the preparation of a plasminogen activator is described, which comprises bringing normal diploid cells derived from human bodies capable of producing the plasminogen activator into contact with a solution containing an enzymatically-decomposed animal meat peptone, the plasminogen activator having the following properties: (a) molecular weight: 63,000±10,000; (b) isoelectric point: 7.0 to 8.5; (c) affinity to fibrin: present; (d) affinity to Concanavalin A: present; (e) optimum pH: 7 to 9.5; and (f) no reactivity with anti-urokinase specific antibody. The plasminogen activator can be obtained in a high yield.
Inventors
- 👤 Akio Hasegawa
- 👤 Hiroshige Kojima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 21:23
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
78 words
A process for the preparation of a plasminogen activator, which comprises bringing normal diploid cells derived from human bodies capable of producing the plasminogen activator into contact with a solution containing an enzymatically-decomposed animal meat peptone, the plasminogen activator having the following properties: (a) molecular weight: 63,000±10,000; (b) isoelectric point: 7.0 to 8.5; (c) affinity to fibrin: present; (d) affinity to Concanavalin A: present; (e) optimum pH: 7 to 9.5; and (f) no reactivity with anti-urokinase specific antibody.
Claim 2
Dependent Claim
78 words
A process for the preparation of a plasminogen activator, which comprises bringing normal diploid cells derived from human bodies capable of producing the plasminogen activator into contact with a solution containing an enzymatically-decomposed animal meat peptone, the plasminogen activator having the following properties: (a) molecular weight: 63,000±10,000; (b) isoelectric point: 7.0 to 8.5; (c) affinity to fibrin: present; (d) affinity to Concanavalin A: present; (e) optimum pH: 7 to 9.5; and (f) no reactivity with anti-urokinase specific antibody.
Claim 3
Dependent Claim
25 words
A process as in claim 1, wherein the peptone concentration of said solution containing the enzymatically-decomposed animal meat peptone is from 0.1 to 4% (wt/v).
Claim 4
Dependent Claim
20 words
A process as in claim 1, wherein said normal diploid cells are cells derived from human lung or human foreskin.
Claim 5
Dependent Claim
25 words
A process as in claim 1, wherein the peptone concentration of said solution containing the enzymatically-decomposed animal meat peptone is from 0.1 to 2% (wt/v).
Claim 6
Dependent Claim
20 words
A process as in claim 2, wherein said normal diploid cells are cells derived from human lung or human foreskin.
Claim 7
Dependent Claim
20 words
A process as in claim 4 wherein said normal diploid cells are cells derived from human lung or human foreskin.
References Cited (Backward Citations)
US3904480A
Cited
US4190708A
Cited
US4232124A
Cited
US4317882A
Cited
GB2104081A
Cited
EP0100982A2
Cited
US4505893A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-10-28 | 1989-04-29 | 1989-10-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-10-28 | 1993-04-29 | 1993-10-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-10-28 | 1997-04-29 | 1997-10-29 | $7700.00 | Window Closed / Lapsed |