US4190708A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of urokinase
Assignee: Monsanto Co
•
Filing: 1978-05-24
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Granted: 1980-02-26
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Expires: 1998-05-24
Abstract
Abstract The yield of urokinase produced by cell culture of kidney cells is improved by incorporating in the cell culture medium an elevated level of phenylalanine.
Inventors
- 👤 Mau-Jung Kuo
- 👤 Margaret J. Reents
- 👤 Joseph Feder
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 22:30
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456; C12N9/6462; C12Y; C12Y304/00; C12Y304/21; C12Y304/21073
Claims Analysis
Total Claims: 5
Claim 1
Independent Claim
51 words
In a process for the production of urokinase by the culture of live kidney cells in an aqueous nutrient medium, the improvement which comprises adding phenylalanine in an elevated amount of from about 0.3 to about 0.7 weight percent to the said nutrient medium to thereby increase the yield of urokinase.
Claim 2
Dependent Claim
51 words
In a process for the production of urokinase by the culture of live kidney cells in an aqueous nutrient medium, the improvement which comprises adding phenylalanine in an elevated amount of from about 0.3 to about 0.7 weight percent to the said nutrient medium to thereby increase the yield of urokinase.
Claim 3
Dependent Claim
19 words
The method of claim 1 in which the phenylalanine is used in an amount of about 0.5 weight percent.
Claim 4
Dependent Claim
16 words
The method of claim 1 in which the cells are normal primary human embryonic kidney cells.
Claim 5
Dependent Claim
19 words
The method of claim 3 in which the medium comprises lactalbumin hydrolysate, human serum albumin, glucose and sodium bicarbonate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-02-25 | 1983-08-27 | 1984-02-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-02-25 | 1987-08-27 | 1988-02-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-02-25 | 1991-08-27 | 1992-02-26 | $7700.00 | Window Closed / Lapsed |