US4232124A
Expired / Lapsed
US
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Method of producing plasminogen activator
Assignee: Hem Research Inc
•
Filing: 1979-04-23
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Granted: 1980-11-04
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Expires: 1999-04-23
Abstract
Abstract Production of plasminogen activator from human diploid lung cells through cultivation in an aqueous nutrient medium containing an inducer of activator production. Lactalbumin hydrolysate is an active inducer, and is used in 0.1-1.0% w/v concentration in the medium.
Inventors
- 👤 George F. Mann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 14:19
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6456
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
57 words
A method for producing plasminogen activator which comprises culturing human diploid lung cells in vitro in an aqueous nutrient maintenance medium free of serum containing therein an inducer which enhances production of plasminogen activator by the cells until plasminogen activator content in the nutrient medium exceeds 100 CTA units/ml and thereafter separating nutrient medium from the cells.
Claim 2
Independent Claim
57 words
A method for producing plasminogen activator which comprises culturing human diploid lung cells in vitro in an aqueous nutrient maintenance medium free of serum containing therein an inducer which enhances production of plasminogen activator by the cells until plasminogen activator content in the nutrient medium exceeds 100 CTA units/ml and thereafter separating nutrient medium from the cells.
Claim 3
Independent Claim
47 words
A method for producing plasminogen activator which comprises culturing human diploid lung cells in vitro in an aqueous nutrient medium containing therein an inducer comprising lactalbumin hydrolysate until plasminogen activator content in the nutrient medium exceeds 100 CTA units/ml and thereafter separating nutrient medium from the cells.
Claim 4
Independent Claim
47 words
A method for producing plasminogen activator which comprises culturing human diploid lung cells in vitro in an aqueous nutrient medium containing therein an inducer comprising lactalbumin hydrolysate until plasminogen activator content in the nutrient medium exceeds 100 CTA units/ml and thereafter separating nutrient medium from the cells.
Claim 5
Independent Claim
63 words
A method for producing plasminogen activator which comprises culturing human embryo diploid lung cells in vitro in an aqueous nutrient medium containing therein an inducer comprising lactalbumin hydrolysate in from 0.1-1.0% w/v of the nutrient medium until plasminogen activator content in the nutrient medium exceeds 100 CTA units/ml, thereafter separating nutrient medium from the cells and then recovering the activator from the medium.
Claim 6
Independent Claim
63 words
A method for producing plasminogen activator which comprises culturing human embryo diploid lung cells in vitro in an aqueous nutrient medium containing therein an inducer comprising lactalbumin hydrolysate in from 0.1-1.0% w/v of the nutrient medium until plasminogen activator content in the nutrient medium exceeds 100 CTA units/ml, thereafter separating nutrient medium from the cells and then recovering the activator from the medium.
Claim 7
Dependent Claim
17 words
The method of claim 2 wherein the lactalbumin hydrolysate is from 0.1-1.0% w/v of the nutrient medium.
Claim 8
Dependent Claim
15 words
The method of claim 1 wherein the plasminogen activator is thereafter recovered from the medium.
Claim 9
Dependent Claim
23 words
The method of claim 3 wherein the medium is changed periodically, whereby multiple harvests of activator are obtained from the same cell culture.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1983-11-04 | 1984-05-05 | 1984-11-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1987-11-04 | 1988-05-05 | 1988-11-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1991-11-04 | 1992-05-05 | 1992-11-04 | $7700.00 | Window Closed / Lapsed |