Patent US6744133B2 successfully refreshed from Google Patents.
Patent US4544631A successfully refreshed from Google Patents.
US4544631A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Process and reagent for the determination of α-amylase
Assignee: Roche Diagnostics GmbH
•
Filing: 1981-10-16
•
Granted: 1985-10-01
•
Expires: 2001-10-16
Abstract
Abstract alpha -Amylase is determined by the enzymatic splitting of an alpha -amylase substrate and measurement of a fission product, wherein there is used as a substrate a maltoheptaose compound of the formula <IMAGE> wherein R is a glucoside, phenylglucoside, mononitrophenylglucoside, dinitrophenylglucoside, sorbitol or gluconic acid group. Reagents comprising such a substrate and a system for the determination of a fission product formed from the amylase substrate by alpha -amylase, are also provided.
Inventors
- 👤 Elli Rauscher
- 👤 Ulrich Neumann
- 👤 August W. Wahlefeld
- 👤 Alexander Hagen
- 👤 Wolfgang Gruber
- 👤 Joachim Ziegenhorn
- 👤 Eugen Schaich
- 👤 Ulfert Deneke
- 👤 Gerhard Michal
- 👤 Gunter Weimann
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 12:53
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/40; C12Q2334/00; C12Q2334/10; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/81
Claims Analysis
Total Claims: 21
Claim 1
Independent Claim
64 words
In a process for the determination of alpha-amylase by the enzymatic splitting of an alpha-amylase substrate and measurement of a fission product, the improvement comprising using, as a substrate, an enzymatically produced maltoheptaose compound of the formula ##STR9## in which R is a phenylglucoside, mononitrophenyl-glucoside, dinitrophenylglucoside, and wherein the sugar moiety of the R radical is substituted in the alpha-configuration, in combination with alpha-glucosidase.
Claim 2
Dependent Claim
64 words
In a process for the determination of alpha-amylase by the enzymatic splitting of an alpha-amylase substrate and measurement of a fission product, the improvement comprising using, as a substrate, an enzymatically produced maltoheptaose compound of the formula ##STR9## in which R is a phenylglucoside, mononitrophenyl-glucoside, dinitrophenylglucoside, and wherein the sugar moiety of the R radical is substituted in the alpha-configuration, in combination with alpha-glucosidase.
Claim 3
Dependent Claim
20 words
Process as claimed in claim 1, wherein said reaction is carried out at a pH value from 5 to 9.
Claim 4
Dependent Claim
17 words
Process as claimed in claim 1, wherein 0.1 to 250 mMol/l. of said maltoheptaose compound are used.
Claim 5
Dependent Claim
17 words
Process as claimed in claim 3, wherein 0.5 to 100 m/Mol.l. of the maltoheptaose compound are used.
Claim 6
Dependent Claim
12 words
Process as claimed in claim 1, wherein R is a phenylglucoside group.
Claim 7
Dependent Claim
29 words
Process as claimed in claim 5, wherein the phenol fission product is determined with 3-methyl-6-sulphonylbenzthiazolone-hydrazone-(2) in the presence of monophenol oxidase, as a measure of the initial α-amylase content.
Claim 8
Dependent Claim
42 words
Composition for use in determination of the presence or lack thereof of alpha amylase comprising an enzymatically produced maltoheptaose compound of claim 1 and a substance which determines the presence of fission products formed by action of alpha amylase on said maltoheptaose.
Claim 9
Dependent Claim
27 words
Composition as claimed in claim 7, consisting essentially of alpha-glucosidase, hexokinase, glucose-6-phosphate dehydrogenase, NAD, ATP, magnesium ions, NaCl, phosphate buffer and an enzymatically produced maltoheptaose as substrate.
Claim 10
Dependent Claim
77 words
Reagent as claimed in claim 8 comprising: 5×10 3 to 3×10 4 U/l. Alpha-glucosidase, 10 3 to 5×10 4 U/l. hexokinase, 10 3 to 5×10 4 U/l. glucose-6-phosphate dehydrogenase, 0.5 to 8 mMol/l. NAD, 0.5 to 5 mMol/l. ATP, 1 to 3 mMol/l. magnesium ions, 25 to 100 mMol/l. sodium chloride or potassium chloride, 25 to 100 mMol/l. buffer (pH 6.2 to 7.8) and 5 to 100 mMol/l. an enzymatically produced maltoheptaose in dry or dissolved form.
Claim 11
Dependent Claim
21 words
Composition as claimed in claim 7, further comprising alpha glucosidase, sodium chloride or potassium chloride, buffer, and an enzymatically produced maltoheptaose.
Claim 12
Dependent Claim
43 words
Composition as claimed in claim 10, comprising: 10 2 to 5×10 6 U/l. α-glucosidase, 1 to 100 mMol/l. sodium chloride or potassium chloride, 10 to 250 mMol/l. buffer (pH 5 to 9), and 0.1 to 250 mMol/l of an enzymatically produced maltoheptaose compound.
Claim 13
Dependent Claim
18 words
Reagent as claimed in claim 10, which additionally contains at least one of β-glucosidase, phenol oxidase and 3-methyl-6-sulphonyl-benzthiazolone-hydrazone-(2).
Claim 14
Dependent Claim
19 words
Reagent as claimed in claim 10, which additionally contains sorbitol dehydrogenase or gluconate kinase and 6-phosphogluconate dehydrogenase, and NAD.
Claim 15
Dependent Claim
16 words
Reagent as claimed in claim 13, which contains in addition a tetrazolium salt, diaphorase or PMS.
Claim 16
Dependent Claim
18 words
Reagent as claimed in claim 13, wherein the reagents are impregnated or incorporated into a sheet-like carrier material.
Claim 17
Dependent Claim
11 words
Reagent as claimed in claim 7, also containing a surface-active agent.
Claim 18
Dependent Claim
26 words
Reagent as claimed in claim 7, consisting essentially of maltase phosphorylase, alpha-phosphoglucomutase, glucose-6-phosphate dehydrogenase, glucose-1, 6-diphosphate, NAD, buffer, and as a substrate an enzymatically produced maltoheptaose.
Claim 19
Dependent Claim
11 words
Composition as in claim 7, wherein said substance is maltose phosphorylase.
Claim 20
Dependent Claim
65 words
In a process for the determination of alpha-amylase by the enzymatic splitting of an alpha-amylase substrate and measurement of a fission product, the improvement comprising using a substrate consisting essentially of an alpha substituted maltoheptaose compound of the formula ##STR10## wherein R is a phenylglucoside, mononitrophenyl-glucoside, dinitrophenylglucose, and wherein the sugar moiety of the R radical is substituted in the alpha-configuration, in combination with alpha-glucosidase.
Claim 21
Dependent Claim
65 words
In a process for the determination of alpha-amylase by the enzymatic splitting of an alpha-amylase substrate and measurement of a fission product, the improvement comprising using a substrate consisting essentially of an alpha substituted maltoheptaose compound of the formula ##STR10## wherein R is a phenylglucoside, mononitrophenyl-glucoside, dinitrophenylglucose, and wherein the sugar moiety of the R radical is substituted in the alpha-configuration, in combination with alpha-glucosidase.
References Cited (Backward Citations)
US3886045A
Cited
US4000042A
Cited
US3867259A
Cited
US4101381A
Cited
US4036697A
Cited
US4059407A
Cited
US4081326A
Cited
US4071407A
Cited
US4102747A
Cited
US4237221A
Cited
US4233403A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-09-30 | 1989-04-01 | 1989-10-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-30 | 1993-04-01 | 1993-10-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-09-30 | 1997-04-01 | 1997-10-01 | $7700.00 | Window Closed / Lapsed |