US4071407A
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Novel maltase enzyme produced by a new yeast strain
Assignee: UAB RESEARCH FOUNDATION NON-PROFIT CORP
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Filing: 1976-11-16
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Granted: 1978-01-31
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Expires: 1996-11-16
Abstract
Abstract A maltase enzyme is prepared by growing a strain of Saccharomyces, ATCC 20 488, on a medium containing maltose or malt extract and has been found to be useful in assays for amylase.
Inventors
- 👤 Leo M. Hall
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 00:27
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2408; C12N9/2411; C08; C08B; C08B30/00; C08B30/12; C08B30/18; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; C12Q1/34; C12Q1/40; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/814; Y10S435/815; Y10S435/8215; Y10S435/911; Y10S435/94
Claims Analysis
Total Claims: 16
Claim 1
Independent Claim
62 words
Maltase enzyme characterized by hydrolyzing maltotetraose only about 1/100 as fast as maltose and maltopentaose only about 1/500 as fast as maltose and prepared by growing a strain of Saccharomyces, ATCC 20,488, in a growth medium containing at least one of maltose, maltotriose or malt extract derived from a plant source, releasing the enzyme from the yeast cells, and recovering the enzyme.
Claim 2
Dependent Claim
62 words
Maltase enzyme characterized by hydrolyzing maltotetraose only about 1/100 as fast as maltose and maltopentaose only about 1/500 as fast as maltose and prepared by growing a strain of Saccharomyces, ATCC 20,488, in a growth medium containing at least one of maltose, maltotriose or malt extract derived from a plant source, releasing the enzyme from the yeast cells, and recovering the enzyme.
Claim 3
Dependent Claim
30 words
The enzyme of claim 1, which is free of nucleic acids, α-and β-amylase, ethanol, ethylacetate, salts, peptides, alcohol dehydrogenase, aldehyde dehydrogenase, hexokinase, glucose-6-phosphate dehydrogenase, protease, NADH oxidase, and other proteins.
Claim 4
Dependent Claim
39 words
A process for prepaing a novel maltase enzyme which comprises growing a novel strain of Saccharomyces, ATCC 20 488, in a growth medium which comprises at least one of maltose, maltotriose or malt extract derived from a plant source.
Claim 5
Dependent Claim
39 words
A process for prepaing a novel maltase enzyme which comprises growing a novel strain of Saccharomyces, ATCC 20 488, in a growth medium which comprises at least one of maltose, maltotriose or malt extract derived from a plant source.
Claim 6
Dependent Claim
18 words
The process of claim 3 wherein the growth medium contains a malt extract derived from a plant source.
Claim 7
Dependent Claim
17 words
The process of claim 3 wherein the growth medium contains a yeast extract, tryptone, and ammonium phosphate.
Claim 8
Dependent Claim
15 words
The process of claim 3, wherein the enzyme is recovered by centrifuging a growing medium.
Claim 9
Dependent Claim
28 words
The process of claim 3, wherein from 2.5 gms/l to 75 gms/l of at least one maltose, maltotriose or malt extract derived from a plant source is present.
Claim 10
Dependent Claim
56 words
The process of claim 7, wherein the growing medium also contains about: 2 to 10 gms/l of tryptone; 1.0 to 4.0 gms/l of (NH 4 ) 2 SO 4 ; 1 to 10 gms/l of KH 2 PO 4 ; 0.10 to 0.40 gms/l of CaCl 2 and 0.10 to 0.40 gms/l of MgSO 4 .
Claim 11
Dependent Claim
15 words
The process of claim 3, wherein an anti-foaming agent is added to the growth medium.
Claim 12
Dependent Claim
29 words
The process of claim 8 whrein from 50 to 60 gms/l of at least one of maltose, maltotriose or a malt extract derived from a plant source is present.
Claim 13
Dependent Claim
20 words
The process of claim 10 wherein a malt extract derived from a plant source is present in the growth medium.
Claim 14
Dependent Claim
53 words
In a method of assaying for the enzyme amylase in a sample by: a. introducing the sample into an assay solution comprising an oligosaccharide and maltase; and b. measuring the rate at which glucose is formed in the assay solution; the improvement which comprises using as the maltase, the maltase of claim 1.
Claim 15
Dependent Claim
20 words
The method of claim 12, wherein the assay solution also comprises hexokinase, glucose-6-phosphate dehydrogenase, adenosine triphosphate, and nicotinamide adenine diniicleotide.
Claim 16
Dependent Claim
27 words
The method of claim 13, wherein the change in absorbance at 340 nm of the sample containing assay solution is taken as the measurement of amylase activity.
References Cited (Backward Citations)
US4000042A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1981-01-30 | 1981-08-01 | 1982-01-31 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1985-01-30 | 1985-08-01 | 1986-01-31 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1989-01-30 | 1989-08-01 | 1990-01-31 | $7700.00 | Window Closed / Lapsed |