Patent US4540667A successfully refreshed from Google Patents.
US4540667A
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Fluorescent siderophore genes
Assignee: University of California
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Filing: 1982-10-12
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Granted: 1985-09-10
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Expires: 2002-10-12
Abstract
Abstract DNA sequences encoding for plant growth promotant activity have been isolated and introduced into microorganisms. The modified organisms are able to confer plant growth promotant activity analogous to that of the DNA source host. Such modified hosts find use in promoting the growth of root crops by innoculating the rhizosphere with such microorganisms. E. coli HB101 (pS FL-1) was deposited at the A.T.C.C. on Oct. 8, 1982, and granted accession no. 39206.
Inventors
- 👤 Cindy Orser
- 👤 Joyce Loper
- 👤 Nickolas Panopoulos
- 👤 Steven Lindow
- 👤 Milton N. Schroth
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 21:28
CPC Classifications
C; C12; C12N; C12N15/00; A; A01; A01N; A01N63/00; A01N63/20; A01N63/27; C12N1/00; C12N1/20; C12N1/205; C12P; C12P1/00; C12P1/04; C12R; C12R2001/00; C12R2001/01; C12R2001/38; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/848; Y10S435/849; Y10S435/874; Y10S435/876; Y10S435/877
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
57 words
A rhizosphere inhabiting bacterial microorganism having enhanced ability to produce a fluorescent siderophore capable of sequestering Fe +3 as a result of in vitro introduction into said microorganism, or into a parent of said microorganism, of a DNA sequence encoding for said siderophone which is expressed by a gene present in pS FL-1 (A.T.C.C. Accession No. 39206).
Claim 2
Dependent Claim
57 words
A rhizosphere inhabiting bacterial microorganism having enhanced ability to produce a fluorescent siderophore capable of sequestering Fe +3 as a result of in vitro introduction into said microorganism, or into a parent of said microorganism, of a DNA sequence encoding for said siderophone which is expressed by a gene present in pS FL-1 (A.T.C.C. Accession No. 39206).
Claim 3
Dependent Claim
16 words
A microorganism according to claim 1, wherein said DNA sequence is incorporated on an extrachromosomal element.
Claim 4
Dependent Claim
15 words
A microorganism according to claim 1, wherein said DNA sequence is integrated into the chromosome.
Claim 5
Dependent Claim
15 words
A microorganism according to claim 1, wherein the microorganism was previously incompetent to produce siderophores.
Claim 6
Dependent Claim
15 words
A microorganism according to claim 1, wherein the microorganism was previously competent to produce siderophores.
Claim 7
Independent Claim
85 words
A method for conferring on a bacterial host the ability to produce a fluorescent siderophore capable of sequestering Fe +3 , said method comprising: screening a gene library obtained from a fluorescent strain of Pseudomonas to obtain a DNA sequence which encodes for said siderophore, wherein said siderophore is encoded for by a DNA sequence present in pS FL-1 (A.T.C.C. Accession No. 39206); incorporating said DNA sequence onto a vector capable of replication and expression in the host; and introducing the vector into the host.
Claim 8
Independent Claim
85 words
A method for conferring on a bacterial host the ability to produce a fluorescent siderophore capable of sequestering Fe +3 , said method comprising: screening a gene library obtained from a fluorescent strain of Pseudomonas to obtain a DNA sequence which encodes for said siderophore, wherein said siderophore is encoded for by a DNA sequence present in pS FL-1 (A.T.C.C. Accession No. 39206); incorporating said DNA sequence onto a vector capable of replication and expression in the host; and introducing the vector into the host.
Claim 9
Dependent Claim
15 words
A method as in claim 6, wherein the vector is incorporated into the host genome.
Claim 10
Dependent Claim
15 words
A method as in claim 6, wherein the vector replicates independently of the host genome.
Claim 11
Dependent Claim
2 words
pS FL-1.
Claim 12
Dependent Claim
2 words
pS FL-1.
References Cited (Backward Citations)
US3819482A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-09-09 | 1989-03-11 | 1989-09-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-09 | 1993-03-11 | 1993-09-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-09-09 | 1997-03-11 | 1997-09-10 | $7700.00 | Window Closed / Lapsed |