US3819482A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of preparing high yields of double-stranded ribonucleic acid
Assignee: ETTEN J VAN
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Filing: 1972-05-08
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Granted: 1974-06-25
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Expires: 1992-05-08
Abstract
Abstract An unusual bacteriophage phi 6, ATCC No. 21781-B, containing a lipid coat and double-stranded ribonucleic acid has been isolated for the plant pathogenic bacterium Pseudomonas phaseolicola ATCC No. 21781. High yields of double-stranded ribonucleic acid may be obtained rapidly and expediently by cultivating a host bacterium for the bacteriophage, infecting the host bacteria with the bacteriophage to provide a lysate and recovering double-stranded ribonucleic acid from the separated bacteriophage. The intact or whole bacteriophage, the bacteriophage with its lipid coat removed, and the isolated nucleic acid have all been found to be effective interferon inducers.
Inventors
- 👤 A Vidaver
- 👤 Etten J Van
- 👤 J Semancik
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 04:05
CPC Classifications
C; C07; C07H; C07H21/00; C12; C12N; C12N7/00; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; C12N2795/00; C12N2795/00011; C12N2795/14011; C12N2795/14211; C12N2795/14251; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/822; Y10S435/874
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
19 words
The method of claim 1 wherein the double-stranded-containing bacteriophage is phage phi 6 having the ATCC reference No. 21781-B.
Claim 2
Dependent Claim
21 words
The method of claim 2 wherein the host bacteria are selected from strains of Pseudomonas phaseolicola, Pseudomonas glycinea and Pseudomonas syringae.
Claim 3
Dependent Claim
17 words
The method of claim 3 wherein the host bacteria are Pseudomonas phaseolicola, having ATCC reference No. 21781.
Claim 4
Dependent Claim
33 words
The method of claim 3 wherein the phage phi 6 is added when the Pseudomonas phaseolicola count is in the range of about 2 -10 X 108 cells per ml of nutrient medium.
Claim 5
Dependent Claim
44 words
The method of claim 2 wherein the recovery of said double-stranded ribonucleic acid comprises adding a deproteinizing solvent to an aqueous dispersion of said separated phage phi 6 to extract protein, centrifuging the mixture and recovering the resulting aqueous phase containing double-stranded ribonucleic acid.
Claim 6
Dependent Claim
31 words
The method of claim 6 wherein prior to said phenol extraction liquid is removed from said phage phi 6 by extraction with a lipid solvent or by treatment with lipid-removing enzymes.
Claim 7
Dependent Claim
16 words
The process of claim 6 wherein the deproteinizing solvent is phenol or phenol sodium dodecyl sulfate.
References Cited (Backward Citations)
US3583893A
Cited
US3582469A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-24 | 1977-12-24 | 1978-06-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-24 | 1981-12-24 | 1982-06-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-24 | 1985-12-24 | 1986-06-25 | $7700.00 | Window Closed / Lapsed |