Patent US4539295A successfully refreshed from Google Patents.
US4539295A
Expired / Lapsed
US
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Binary kinetic assay method and apparatus
Assignee: Beckman Coulter Inc
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Filing: 1983-06-30
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Granted: 1985-09-03
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Expires: 2003-06-30
Abstract
Abstract A kinetic assay method and apparatus for performing such method wherein a first sample containing predetermined concentrations of binary analytes is reacted with the reagent to form a reaction product. Reaction rates at first and second times are measured for the first reaction and a first ratio between such measured reaction rates is determined. A second reaction with a second sample and the reagent is also performed wherein the second sample contains predetermined concentrations of the binary analytes. Reaction rates at the first and second times are measured for the second reaction and a second ratio between such rates is determined. A third reaction with a third sample and the reagent is performed, the sample including unknown relative concentrations of the first and second analytes. Reaction rates are again measured at the first and second times and a third ratio between such measured reaction rates is determined. By comparing the first and third ratios, an indication can be provided to show that the reaction is not influenced by an interfering analyte. The third ratio may be compared to a range of ratios defined by the first and second ratios to determine the relative contribution of at least one of the analytes to a reaction product formed during the third reaction. The relative contribution of a selected one of the analytes may be used to determine a concentration of such analyte corrected for the influence or bias of the other analyte in the reaction.
Inventors
- 👤 William M. Blough, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 23:42
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/70; C; C12; C12Q; C12Q1/00; G01N21/00; G01N21/17; G01N21/25; G01N21/27; G01N21/272
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
158 words
A method for providing an indication that a reaction between a sample containing at least a first analyte and a reagent is influenced by the presence of at least a second analyte in the sample also reacting with the reagent but at a rate different from the first analyte comprising the steps of: performing a first reaction with only the first analyte and the reagent, measuring first and second reaction rates at respective first and second times during the first reaction, and forming a first ratio between the first and second reaction rates; performing a second reaction with the sample and the reagent, measuring third and fourth reaction rates at the first and second times, respectively, during the second reaction, and forming a second ratio between the third and fourth reaction rates; and comparing the first ratio and the second ratio and providing the indication if the second ratio is in a predetermined relationship with the first ratio.
Claim 2
Independent Claim
158 words
A method for providing an indication that a reaction between a sample containing at least a first analyte and a reagent is influenced by the presence of at least a second analyte in the sample also reacting with the reagent but at a rate different from the first analyte comprising the steps of: performing a first reaction with only the first analyte and the reagent, measuring first and second reaction rates at respective first and second times during the first reaction, and forming a first ratio between the first and second reaction rates; performing a second reaction with the sample and the reagent, measuring third and fourth reaction rates at the first and second times, respectively, during the second reaction, and forming a second ratio between the third and fourth reaction rates; and comparing the first ratio and the second ratio and providing the indication if the second ratio is in a predetermined relationship with the first ratio.
Claim 3
Dependent Claim
47 words
A method as in claim 1 wherein the step of performing the first reaction includes selecting the first and second times such that one of such times corresponds substantially to a time that the reaction rate for the first analyte reaches a peak during the first reaction.
Claim 4
Dependent Claim
33 words
A method as in claim 1 wherein the step of comparing the first and second ratios includes providing the indication if the second ratio differs from the first ratio by a predetermined amount.
Claim 5
Dependent Claim
36 words
A method as in claim 1 wherein the step of comparing the first and second ratios includes providing the indication if the second ratio is within a predetermined range of ratios related to the first ratio.
Claim 6
Independent Claim
194 words
A method for providing an indication that a reaction between a sample containing at least a first analyte and a reagent is influenced by the presence of at least a second analyte in the sample also reacting with the reagent but at a rate different from the first analyte comprising the steps of: performing a first reaction with only the first analyte and the reagent, selecting first and second times for reaction rate measurements such that one of such times corresponds substantially to a time that the reaction rate of the first analyte reaches a peak during the first reaction, measuring first and second reaction rates at the first and second times, respectively, during the first reaction and forming a first ratio between the first and second reaction rates; performing a second reaction with the sample and the reagent, measuring third and fourth reaction rates at the first and second times, respectively, during the second reaction, and forming a second ratio between the third and fourth reaction rates; and comparing the first ratio and the second ratio and providing the indication if the second ratio differs from the first ratio by a predetermined amount.
Claim 7
Independent Claim
194 words
A method for providing an indication that a reaction between a sample containing at least a first analyte and a reagent is influenced by the presence of at least a second analyte in the sample also reacting with the reagent but at a rate different from the first analyte comprising the steps of: performing a first reaction with only the first analyte and the reagent, selecting first and second times for reaction rate measurements such that one of such times corresponds substantially to a time that the reaction rate of the first analyte reaches a peak during the first reaction, measuring first and second reaction rates at the first and second times, respectively, during the first reaction and forming a first ratio between the first and second reaction rates; performing a second reaction with the sample and the reagent, measuring third and fourth reaction rates at the first and second times, respectively, during the second reaction, and forming a second ratio between the third and fourth reaction rates; and comparing the first ratio and the second ratio and providing the indication if the second ratio differs from the first ratio by a predetermined amount.
Claim 8
Independent Claim
168 words
An apparatus for providing an indication that a reaction between a sample containing at least a first analyte and a reagent is influenced by the presence of at least a second analyte in the sample also reacting with the reagent but at a rate different from the first analyte comprising: means for performing a first reaction with only a first analyte and a reagent; means for measuring first and second reaction rates at respective first and second times during the first reaction; means for forming a first ratio between the first and second reaction rates; means for performing a second reaction with a sample and the reagent; means for measuring third and fourth reaction rates at the first and second times, respectively, during the second reaction; means for forming a second ratio between the third and fourth reaction rates; means for comparing the first ratio and the second ratio; and means for providing the indication if the second ratio is in a predetermined relationship with the first ratio.
Claim 9
Independent Claim
168 words
An apparatus for providing an indication that a reaction between a sample containing at least a first analyte and a reagent is influenced by the presence of at least a second analyte in the sample also reacting with the reagent but at a rate different from the first analyte comprising: means for performing a first reaction with only a first analyte and a reagent; means for measuring first and second reaction rates at respective first and second times during the first reaction; means for forming a first ratio between the first and second reaction rates; means for performing a second reaction with a sample and the reagent; means for measuring third and fourth reaction rates at the first and second times, respectively, during the second reaction; means for forming a second ratio between the third and fourth reaction rates; means for comparing the first ratio and the second ratio; and means for providing the indication if the second ratio is in a predetermined relationship with the first ratio.
Claim 10
Dependent Claim
44 words
An apparatus as in claim 6 wherein the apparatus includes means for fixing the first and second times such that one of such times corresponds substantially to a time that the reaction rate for the first analyte reaches a peak during the first reaction.
Claim 11
Dependent Claim
28 words
An apparatus as in claim 6 wherein the apparatus includes means for providing the indication if the second ratio differs from the first ratio by a predetermined amount.
Claim 12
Dependent Claim
32 words
An apparatus as in claim 6 wherein the apparatus includes means for providing the indication if the second ratio is within a predetermined range of rate ratios related to the first ratio.
Claim 13
Independent Claim
206 words
An apparatus for providing an indication that a reaction between a sample containing at least a first analyte and a reagent is influenced by the presence of at least a second analyte in the sample also reacting with the reagent but at a rate different from the first analyte comprising: means for performing a first reaction with only a first analyte and a reagent; means for fixing first and second times for reaction rate measurements such that one of such times corresponds substantially to a time that the reaction rate of the first analyte reaches a peak during the first reaction; means for measuring first and second reaction rates at the first and second times, respectively, during the first reaction; means for forming a first ratio between the first and second reaction rates; means for performing a second reaction with a sample and the reagent; means for measuring third and fourth reaction rates at the first and second times, respectively, during the second reaction; means for forming a second ratio between the third and fourth reaction rates; means for comparing the first ratio and the second ratio; and means for providing the indication if the second ratio differs from the first ratio by a predetermined amount.
Claim 14
Independent Claim
206 words
An apparatus for providing an indication that a reaction between a sample containing at least a first analyte and a reagent is influenced by the presence of at least a second analyte in the sample also reacting with the reagent but at a rate different from the first analyte comprising: means for performing a first reaction with only a first analyte and a reagent; means for fixing first and second times for reaction rate measurements such that one of such times corresponds substantially to a time that the reaction rate of the first analyte reaches a peak during the first reaction; means for measuring first and second reaction rates at the first and second times, respectively, during the first reaction; means for forming a first ratio between the first and second reaction rates; means for performing a second reaction with a sample and the reagent; means for measuring third and fourth reaction rates at the first and second times, respectively, during the second reaction; means for forming a second ratio between the third and fourth reaction rates; means for comparing the first ratio and the second ratio; and means for providing the indication if the second ratio differs from the first ratio by a predetermined amount.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-09-02 | 1989-03-04 | 1989-09-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-09-02 | 1993-03-04 | 1993-09-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-09-02 | 1997-03-04 | 1997-09-03 | $7700.00 | Window Closed / Lapsed |