US3881992A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Optical rate measurement method
Assignee: Individual
•
Filing: 1973-07-02
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Granted: 1975-05-06
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Expires: 1993-07-02
Abstract
Abstract A method for clinical analysis is provided by combining a reagent with a sample composition and causing an enzymatic reaction thereof, projecting a light beam at the composition as the reaction progresses, providing a first electrical signal which is proportional to the absorbance of light by the composition, providing a second electrical signal which is substantially proportional to the first derivative of the first absorbance signal, continuously averaging the second signal for a predetermined interval of time, and coupling the second signal to an indicating means for providing a continuous visible display representative of the rate of reaction.
Inventors
- 👤 Wilson Ralston
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 19:21
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/25; G01N21/27; G01N21/272; Y; Y10; Y10S; Y10S435/00; Y10S435/808
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
191 words
A METHOD FOR KINETIC ENZYME ANALYSIS FOR RAPIDLY PROVIDING AN INDICATION OF THE LINEARITY OF ENZYME REACTION COMPRISING: COMBINING A REAGENT WITH A SAMPLE COMPOSITION FOR CAUSING AN ENZYME CATALYZED REACTION THEREOF, SAID REACTION EXHIBITING A LAG PHASE, AN INTERMEDIATE REACTION PHASE AND A TERMINATING SUBSTRATE EXHAUSTION PHASE, PROJECTING A LIGHT BEAM AT THE COMPOSITION AS THE REACTION PROGRESSES, DETECTING THE ABSORBANCE OF SAID LIGHT BEAM AND PROVIDING A FIRST ELECTRICAL SIGNAL WHICH IS PROPORTIONAL TO THE ABSORBANCE OF THE LIGHT BY THE COMPOSITION UNDERGOING REACTION, APPLYING SAID FIRST SIGNAL TO SIGNAL FILTERING AND DIFFERENTIATING MEANS FOR PROVIDING A SECOND ELECTRICAL SIGNAL WHICH IS SUBSTANTIALLY PROPORTIONAL TO THE FIRST DERIVATIVE OF THE FIRST ABSORBANCE SIGNAL, CAUSING REACTIVE ELEMENTS OF A SIGNAL FILTERING AND DIFFERENTIATING MEANS TO EQUILIBRATE TO A ZERO RATE CONDITION PRIOR TO ANALYSIS, CONTINUOUSLY AVERAGING THE SECOND SIGNAL FOR A PREDETERMINED INTERVAL OF TIME FROM SAID ZERO RATE CONDITION IN ORDER TO SUPPRESS UNDESIRED RANDOMLY OCCURING NOISE COMPONENTS, AND, APPLYING THE SECOND SIGNAL TO AN INDICATING MEANS AND PROVIDING A CONTINUOUS VISUAL DISPLAY WHICH, AFTER SAID INTERVAL OF TIME, IS REPRESENTATIVE OF THE RATE OF CHANGE OF THE REACTION.
Claim 2
Independent Claim
191 words
A METHOD FOR KINETIC ENZYME ANALYSIS FOR RAPIDLY PROVIDING AN INDICATION OF THE LINEARITY OF ENZYME REACTION COMPRISING: COMBINING A REAGENT WITH A SAMPLE COMPOSITION FOR CAUSING AN ENZYME CATALYZED REACTION THEREOF, SAID REACTION EXHIBITING A LAG PHASE, AN INTERMEDIATE REACTION PHASE AND A TERMINATING SUBSTRATE EXHAUSTION PHASE, PROJECTING A LIGHT BEAM AT THE COMPOSITION AS THE REACTION PROGRESSES, DETECTING THE ABSORBANCE OF SAID LIGHT BEAM AND PROVIDING A FIRST ELECTRICAL SIGNAL WHICH IS PROPORTIONAL TO THE ABSORBANCE OF THE LIGHT BY THE COMPOSITION UNDERGOING REACTION, APPLYING SAID FIRST SIGNAL TO SIGNAL FILTERING AND DIFFERENTIATING MEANS FOR PROVIDING A SECOND ELECTRICAL SIGNAL WHICH IS SUBSTANTIALLY PROPORTIONAL TO THE FIRST DERIVATIVE OF THE FIRST ABSORBANCE SIGNAL, CAUSING REACTIVE ELEMENTS OF A SIGNAL FILTERING AND DIFFERENTIATING MEANS TO EQUILIBRATE TO A ZERO RATE CONDITION PRIOR TO ANALYSIS, CONTINUOUSLY AVERAGING THE SECOND SIGNAL FOR A PREDETERMINED INTERVAL OF TIME FROM SAID ZERO RATE CONDITION IN ORDER TO SUPPRESS UNDESIRED RANDOMLY OCCURING NOISE COMPONENTS, AND, APPLYING THE SECOND SIGNAL TO AN INDICATING MEANS AND PROVIDING A CONTINUOUS VISUAL DISPLAY WHICH, AFTER SAID INTERVAL OF TIME, IS REPRESENTATIVE OF THE RATE OF CHANGE OF THE REACTION.
Claim 3
Dependent Claim
44 words
The method of claim 1 wherein said second electrical signal is provided and averaged by circuit means including an active filter having reactive components and including the step of equilizing the reactive elements to a zero rate condition prior to initiation of the analysis.
Claim 4
Dependent Claim
37 words
The method of claim 2 including the step of actuating a controlling means for equalizing said reactive elements and for releasing said controlling means for allowing said second signal to build up to said true rate indication.
Claim 5
Dependent Claim
17 words
The method of claim 1 including the step of damping said second electrical signal near critical damping.
Claim 6
Dependent Claim
19 words
The method of claim 1 wherein said second electrical signal is damped with a damping factor of about 0.85-0.90.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-05-05 | 1978-11-04 | 1979-05-06 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-05-05 | 1982-11-04 | 1983-05-06 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-05-05 | 1986-11-04 | 1987-05-06 | $7700.00 | Window Closed / Lapsed |