Reaction detector
Abstract
Abstract A REACTION DETECTOR MEASURES THE RATE OF REACTION IN A SUBSTANCE UNDERGOING A REACTION, SUCH AS AN ENZYMECATALYZED CHEMICAL REACTION, BY MEASURING THE RATE OF CHANGE OF ABSORBANCE IN THE SUBSTANCE DURING THE REACTION. THIS IS ACCOMPLISHED BY COMPARING THE INTENSITIES OF LIGHT PENETRATING THROUGH A REFERENCE AND THROUGH THE SAMPLE SUBSTANCE UNDERGOING THE REACTION. THE DIFFERENCE BETWEEN THE TWO INTENSITIES IS MEASURED AND THIS DIFFERENCE IS UTILIZED TO MAKE THE AMPLITUDE OF A SIGNAL DERIVED FROM THE REFERENCE SUBSTANTIALLY EQUAL TO THE AMPLITUDE OF THE SIGNAL DERIVED FROM THE SAMPLE SUBSTANCE. THE REFERENCE SIGNAL IS KEPT CONSTANT TO ESTABLISH A REFERENCE LEVEL WHEREAS THE SAMPLE SIGNAL IS MEASURED A PREDETERMINED TIME LATER AND SUCH A MEASUREMENT PROVIDES DIRECTLY THE REACTION RATE. THIS REACTION RATE IS THEN AUTOMATICALLY TRANSFORMED INTO UNITS SPECIFYING THE CONCENTRATION OF ENZYMES IN THE SAMPLE SUBSTANCE. D R A W I N G
Inventors
- 👤 H Marshall
- 👤 J Smeaton
Bibliographic Details
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