Patent US4522922A successfully refreshed from Google Patents.
US4522922A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Soluble assay
Assignee: Individual
•
Filing: 1982-04-16
•
Granted: 1985-06-11
•
Expires: 2002-04-16
Abstract
Abstract Ligand having at least two determinant or binding sites is contacted with a labeled first binder, an unlabeled second binder different than the first binder and a precipitating binder specific for the second binder, with such contacting precipitating a complex of the ligand and the first and second binders to thereby separate bound labeled binder from unbound labeled binder. The bound and/or unbound labeled binder is determined as a measure of the ligand.
Inventors
- 👤 Jose Carro
- 👤 Frank de Velasco
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 06:38
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/536; G01N33/537; G01N33/539; G01N33/541; Y; Y10; Y10S; Y10S435/00; Y10S435/975; Y10S436/00; Y10S436/804; Y10S436/807; Y10S436/808; Y10S436/809; Y10S436/811; Y10S436/815; Y10S436/817; Y10S436/82; Y10S436/828
Claims Analysis
Total Claims: 29
Claim 1
Independent Claim
192 words
A process for the assay of a ligand having at least two determinant sites, comprising: contacting in solution a sample containing said ligand with a first unsupported binder, in labeled form, which is specific for the ligand, said first binder being a member selected from the group consisting of a first antibody and a first naturally occurring binder, a second unsupported binder, which is unlabeled, and which is specific for the ligand, said second binder being a member selected from the group consisting of a second antibody and a second naturally occurring binder, said first and second antibody being raised in different animal species, and said first and second naturally occurring binders being different substances, and an unsupported soluble precipitating binder specific for said second binder, said contacting binding a portion of the first binder and at least a portion of the second binder to the ligand and precipitating a complex comprising the precipitating binder and ligand bound to both the first binder and second binder; and determining the amount of first binder in at least one of the solution and the complex as a measure of the ligand in the sample.
Claim 2
Dependent Claim
192 words
A process for the assay of a ligand having at least two determinant sites, comprising: contacting in solution a sample containing said ligand with a first unsupported binder, in labeled form, which is specific for the ligand, said first binder being a member selected from the group consisting of a first antibody and a first naturally occurring binder, a second unsupported binder, which is unlabeled, and which is specific for the ligand, said second binder being a member selected from the group consisting of a second antibody and a second naturally occurring binder, said first and second antibody being raised in different animal species, and said first and second naturally occurring binders being different substances, and an unsupported soluble precipitating binder specific for said second binder, said contacting binding a portion of the first binder and at least a portion of the second binder to the ligand and precipitating a complex comprising the precipitating binder and ligand bound to both the first binder and second binder; and determining the amount of first binder in at least one of the solution and the complex as a measure of the ligand in the sample.
Claim 3
Dependent Claim
14 words
The process of claim 1 wherein the first and second binders are each antibodies.
Claim 4
Dependent Claim
11 words
The process of claim 2 wherein the ligand is an antigen.
Claim 5
Dependent Claim
34 words
The process of claim 3 wherein the ligand is selected from the group consisting of HCG, insulin, human thyroid stimulating hormone, ferritin, hepatitis associated antigens A and B, alpha-fetoprotein, thyroid stimulating hormone and CEA.
Claim 6
Dependent Claim
23 words
The process of claim 2 wherein the precipitating binder is a precipitating antibody which reacts with the antigenic sites of the second binder.
Claim 7
Dependent Claim
14 words
The process of claim 5 wherein the first antibody is labeled with a radioisotope.
Claim 8
Dependent Claim
13 words
The process of claim 6 wherein the radioisotope is a radioisotope of iodine.
Claim 9
Dependent Claim
10 words
The process of claim 7 wherein the ligand is TSH.
Claim 10
Dependent Claim
10 words
The process of claim 7 wherein the ligand is ferritin.
Claim 11
Dependent Claim
12 words
The process of claim 7 wherein the ligand is hepatitis associated antigen.
Claim 12
Dependent Claim
17 words
The process of claim 1 wherein the first and second binders are sequentially contacted with the sample.
Claim 13
Dependent Claim
17 words
The process of claim 1 wherein the first and second binders are simultaneously contacted with the sample.
Claim 14
Dependent Claim
18 words
The process of claim 1 wherein the first and precipitating binders are simultaneously contacted with the second binder.
Claim 15
Dependent Claim
123 words
A reagent kit for the assay of a ligand having at least two determinant sites, comprising: in reagent containers, an unsupported first binder, in labeled form, specific for said ligand, said first binder being a member selected from the group consisting of a first antibody and a first naturally occurring binder; an unsupported second binder which is unlabeled and which is specific for the ligand to be assayed, said second binder being a member selected from the group consisting of a second antibody and a second naturally occurring binder, said first and second anitbody being antibodies raised in different animal species, and said first and second naturally occurring binders being different substances; and an unsupported soluble precipitating binder specific for said second binder.
Claim 16
Dependent Claim
123 words
A reagent kit for the assay of a ligand having at least two determinant sites, comprising: in reagent containers, an unsupported first binder, in labeled form, specific for said ligand, said first binder being a member selected from the group consisting of a first antibody and a first naturally occurring binder; an unsupported second binder which is unlabeled and which is specific for the ligand to be assayed, said second binder being a member selected from the group consisting of a second antibody and a second naturally occurring binder, said first and second anitbody being antibodies raised in different animal species, and said first and second naturally occurring binders being different substances; and an unsupported soluble precipitating binder specific for said second binder.
Claim 17
Dependent Claim
15 words
The reagent kit of claim 14 wherein the first and second binders are each antibodies.
Claim 18
Dependent Claim
24 words
The reagant kit of claim 15 wherein the precipitating binder is a precipitating antibody which reacts with the antigenic sites of the second binder.
Claim 19
Dependent Claim
15 words
The reagant kit of claim 16 wherein the first antibody is labeled with a radioisotope.
Claim 20
Dependent Claim
14 words
The reagant kit of claim 17 wherein the radioisotope is a radioisotope of iodine.
Claim 21
Dependent Claim
11 words
The reagant kit of claim 18 wherein the ligand is TSH.
Claim 22
Dependent Claim
11 words
The reagent kit of claim 18 wherein the ligand is ferritin.
Claim 23
Dependent Claim
13 words
The reagent kit of claim 18 wherein the ligand is hepatitis associated antigen.
Claim 24
Dependent Claim
18 words
The reagent kit of claim 14 wherein the first and second binders are in a single reagent container.
Claim 25
Dependent Claim
17 words
The reagent kit of claim 14 wherein the first and second binders are in separate reagent containers.
Claim 26
Dependent Claim
119 words
An assay for human ferritin, comprising: contacting a serum sample containing human ferritin with a tracer comprising unsupported ferritin antibody labeled with a radioactive isotope, unsupported unlabeled ferritin antiserum, said labeled antibody and unlabeled antiserum being raised in different animal species, and an unsupported soluble precipitating antibody specific for the unlabeled ferritin antiserum, said contacting binding a portion of the tracer and at least a portion of the ferritin antiserum to the ferritin in the sample and precipitating a complex comprising the precipitating antibody and ferritin bound to both the tracer and unlabeled ferritin antiserum; and determining the amount of tracer in at least one of the solution and the complex as a measure of ferritin in the serum.
Claim 27
Dependent Claim
119 words
An assay for human ferritin, comprising: contacting a serum sample containing human ferritin with a tracer comprising unsupported ferritin antibody labeled with a radioactive isotope, unsupported unlabeled ferritin antiserum, said labeled antibody and unlabeled antiserum being raised in different animal species, and an unsupported soluble precipitating antibody specific for the unlabeled ferritin antiserum, said contacting binding a portion of the tracer and at least a portion of the ferritin antiserum to the ferritin in the sample and precipitating a complex comprising the precipitating antibody and ferritin bound to both the tracer and unlabeled ferritin antiserum; and determining the amount of tracer in at least one of the solution and the complex as a measure of ferritin in the serum.
Claim 28
Dependent Claim
19 words
The process of claim 24 wherein the solution and complex are separated from each other prior to said determining.
Claim 29
Dependent Claim
30 words
The assay of claim 25 wherein the ferritin antibody is raised in rabbit, the ferritin antiserum is raised in goat, and the precipitating antibody is rabbit antiserum to goat IgG.
References Cited (Backward Citations)
US3555143A
Cited
US3935074A
Cited
US4034474A
Cited
US4199559A
Cited
US4343896A
Cited
US4248965A
Cited
US4098876A
Cited
US4088746A
Cited
US4130462A
Cited
US4278652A
Cited
US4297494A
Cited
US4244940A
Cited
US4298592A
Cited
US4281061A
Cited
US4353982A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-06-10 | 1988-12-10 | 1989-06-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-06-10 | 1992-12-10 | 1993-06-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-06-10 | 1996-12-10 | 1997-06-11 | $7700.00 | Window Closed / Lapsed |