Patent US4521334A successfully refreshed from Google Patents.
US4521334A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Synthetic polypeptide fragments
Assignee: University of Tennessee Research Foundation
•
Filing: 1983-06-10
•
Granted: 1985-06-04
•
Expires: 2003-06-10
Abstract
Abstract Peptide fragments of streptococcal M protein named CB3, CB4, and CB5 and related synthetic peptides have been identified. When linked to a protein carrier which is polylysine the conjugates of CB3, CB4, CB5, and S-CB3 are capable of evoking type specific opsonic antibodies against streptococci.
Inventors
- 👤 Edwin H. Beachey
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-09 03:47
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/02; A61K39/09; A61K39/092; Y; Y10; Y10S; Y10S530/00; Y10S530/806
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
102 words
A polypeptide which elicits opsonic antibodies to Streptococcus pyogenes and which is not serologically cross-reactive with tissue antigens of the heart, which polypeptide comprises the amino acid sequence Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-A-Ala-B-C-Ala-D-Leu-E-F-Ala-Leu-Glu-G-H-I wherein A is Glu or Ala B is Glu or Arg C is Lys or Gln D is Asp or Glu E is Glu or Asp F is Lys or Asp G is Gly or Leu H is Ala or Glu I is Hse, Met, Gly-Ala-Hse, or Gly-Ala-Met, with the proviso that the polypeptide is not any one of the four following sequences: Ans-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys-Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Ala-Ala-Arg-Lys-Ala-Asp-Leu-Glu-Lys-Ala-Leu-Glu-Gly-Ala-I, or Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys-Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Glu-Ala-Arg-Gln-Ala-Glu-Leu-Glu-Lys-Ala-Leu-Glu-Gly-Ala-I, wherein I is Met or Hse.
Claim 2
Dependent Claim
102 words
A polypeptide which elicits opsonic antibodies to Streptococcus pyogenes and which is not serologically cross-reactive with tissue antigens of the heart, which polypeptide comprises the amino acid sequence Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-A-Ala-B-C-Ala-D-Leu-E-F-Ala-Leu-Glu-G-H-I wherein A is Glu or Ala B is Glu or Arg C is Lys or Gln D is Asp or Glu E is Glu or Asp F is Lys or Asp G is Gly or Leu H is Ala or Glu I is Hse, Met, Gly-Ala-Hse, or Gly-Ala-Met, with the proviso that the polypeptide is not any one of the four following sequences: Ans-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys-Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Ala-Ala-Arg-Lys-Ala-Asp-Leu-Glu-Lys-Ala-Leu-Glu-Gly-Ala-I, or Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys-Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Glu-Ala-Arg-Gln-Ala-Glu-Leu-Glu-Lys-Ala-Leu-Glu-Gly-Ala-I, wherein I is Met or Hse.
Claim 3
Dependent Claim
12 words
The polypeptide of claim 1 wherein the amino acid sequence is Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys-Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Glu-Ala-Glu-Lys-Ala-Asp-Leu-Glu-Lys-Ala-Leu-Glu-Gly-Ala-Hse.
Claim 4
Dependent Claim
12 words
The polypeptide of claim 1 wherein the amino acid sequence is Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys-Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Glu-Ala-Arg-Gln-Ala-Asp-Leu-Glu-Lys-Ala-Leu-Glu-Gly-Ala-Hse.
Claim 5
Dependent Claim
12 words
The polypeptide of claim 1 wherein the amino acid sequence is Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys-Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Glu-Ala-Arg-Lys-Ala-Asp-Leu-Asp-Asp-Ala-Leu-Glu-Leu-Glu-Gly-Ala-Hse.
Claim 6
Dependent Claim
12 words
The polypeptide of claim 1 wherein the amino acid sequence is Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys-Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Glu-Ala-Gln-Lys-Ala-Asp-Leu-Glu-Lys-Ala-Leu-Glu-Gly-Ala-Met.
Claim 7
Dependent Claim
33 words
A synthetic polypeptide which is able to elicit opsonic antibodies to Streptococcus pyogenes and which is not serologically cross-reactive with tissue antigens of the heart which has the following amino acid sequence Ala-Val-Thr-Lys-Gly-Thr-Ile-Asn-Asp-Pro-Gln-Ala-Ala-Lys-Glu-Ala-Leu-Asp-Lys-Tyr.
Claim 8
Dependent Claim
33 words
A synthetic polypeptide which is able to elicit opsonic antibodies to Streptococcus pyogenes and which is not serologically cross-reactive with tissue antigens of the heart which has the following amino acid sequence Ala-Val-Thr-Lys-Gly-Thr-Ile-Asn-Asp-Pro-Gln-Ala-Ala-Lys-Glu-Ala-Leu-Asp-Lys-Tyr.
Claim 9
Dependent Claim
68 words
A polypeptide which elicits opsonic antibodies to Streptococcus pyogenes and which is not serologically cross-reactive with tissue antigens of the heart, which polypeptide comprises the amino acid sequence Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Glu-Ala-B-C-Ala-Asp-Leu-E-F-Ala-Leu-Glu-G-H-I wherein B is Glu or Arg C is Lys or Gln E is Glu or Asp F is Lys or Asp G is Gly or Leu H is Ala or Glu I is Hse, Met, Gly-Ala-Hse, or Gly-Ala-Met.
Claim 10
Dependent Claim
68 words
A polypeptide which elicits opsonic antibodies to Streptococcus pyogenes and which is not serologically cross-reactive with tissue antigens of the heart, which polypeptide comprises the amino acid sequence Asn-Phe-Ser-Thr-Ala-Asp-Ser-Ala-Lys Ile-Lys-Thr-Leu-Glu-Ala-Glu-Lys-Ala-Ala-Leu-Glu-Ala-B-C-Ala-Asp-Leu-E-F-Ala-Leu-Glu-G-H-I wherein B is Glu or Arg C is Lys or Gln E is Glu or Asp F is Lys or Asp G is Gly or Leu H is Ala or Glu I is Hse, Met, Gly-Ala-Hse, or Gly-Ala-Met.
References Cited (Backward Citations)
US4284537A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-06-03 | 1988-12-03 | 1989-06-04 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-06-03 | 1992-12-03 | 1993-06-04 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-06-03 | 1996-12-03 | 1997-06-04 | $7700.00 | Window Closed / Lapsed |