US4510084A
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US
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Method of producing an antithrombin III-heparin concentrate or antithrombin III-heparinoid concentrate
Assignee: FIRM IMMUNO AG fur CHEMISCH-MEDIZINISCHE PRODUKTE A Co OF AUSTRIA
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Filing: 1984-05-18
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Granted: 1985-04-09
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Expires: 2004-05-18
Abstract
Abstract In a method of producing an antithrombin III-heparin or an antithrombin III-heparinoid concentrate, heparin or heparinoid is added to human plasma or to antithrombin III containing plasma fractions to form an antithrombin III-heparin or antithrombin III-heparinoid complex, the complex thus formed is adsorbed on an anion exchanger, and eluted with a salt solution; then the antithrombin III-heparin or antithrombin III-heparinoid complex contained in the eluate is separated from salts and undesired proteins, and the resulting product is loyphilized.
Inventors
- 👤 Johann Eibl
- 👤 Ernst Hetzl
- 👤 Yendra Linnau
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 09:42
CPC Classifications
A; A61; A61K; A61K31/00; A61K31/70; A61K31/715; A61P; A61P7/00; A61P7/02; C; C07; C07K; C07K14/00; C07K14/81; C07K14/8107; C07K14/811; C07K14/8121; C07K14/8128; C08; C08B; C08B37/00; C08B37/006; C08B37/0063; C08B37/0075; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/829; Y10S530/83
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
105 words
In a method of producing an antithrombin III-heparin concentrate or an antithrombin III-heparinoid concentrate, which method includes adding heparin or heparinoid to human plasma or to antithrombin III-containing plasma fractions under formation of an antithrombin III-heparin complex or an antithrombin III-heparinoid complex, the improvement comprising adsorbing the antithrombin III-heparin complex or the antithrombin III-heparinoid complex formed on an anion exchanger thus forming an adsorbate, eluting said adsorbate with a salt solution thus forming an eluate, separating said antithrombin III-heparin or antithrombin III-heparinoid complex contained in said eluate from salts and undesired proteins, and lyophilizing the resulting product so as to bring it into stable form.
Claim 2
Dependent Claim
105 words
In a method of producing an antithrombin III-heparin concentrate or an antithrombin III-heparinoid concentrate, which method includes adding heparin or heparinoid to human plasma or to antithrombin III-containing plasma fractions under formation of an antithrombin III-heparin complex or an antithrombin III-heparinoid complex, the improvement comprising adsorbing the antithrombin III-heparin complex or the antithrombin III-heparinoid complex formed on an anion exchanger thus forming an adsorbate, eluting said adsorbate with a salt solution thus forming an eluate, separating said antithrombin III-heparin or antithrombin III-heparinoid complex contained in said eluate from salts and undesired proteins, and lyophilizing the resulting product so as to bring it into stable form.
Claim 3
Dependent Claim
20 words
A method as set forth in claim 1, further comprising the step of washing said adsorbate before eluting said adsorbate.
Claim 4
Dependent Claim
48 words
A method as set forth in claim 1, wherein said eluate is treated with protein precipitating agents, in a concentration sufficing to form an antithrombin III-heparin-complex-precipitate or an antithrombin III-heparinoid-complex-precipitate, said precipitate is dissolved so as to form a solution, said solution is thermally inactivated and subsequently lyophilized.
Claim 5
Dependent Claim
18 words
A method as set forth in claim 3, wherein said protein precipitating agents are comprised of ammonium sulfate.
Claim 6
Dependent Claim
55 words
A method as set forth in claim 3, wherein after thermally inactivating said solution, said solution containing said antithrombin III-heparin complex or said antithrombin III-heparinoid complex is further treated with a protein precipitating agent in a concentration sufficing to remove undesired proteins but leaving said antithrombin III-heparin complex or said antithrombin III-heparinoid complex in solution.
Claim 7
Dependent Claim
29 words
A method as set forth in claim 5, wherein said protein precipitating agent for further treating said antithrombin III-heparin complex or antithrombin III-heparinoid complex is comprised of ammonium sulfate.
Claim 8
Independent Claim
108 words
A method of producing an antithrombin III concentrate from said antithrombin III-heparin concentrate or from said antithrombin III-heparinoid concentrate produced as set forth in claims 1, wherein a purified solution enriched with said antithrombin III-heparin complex or said antithrombin III-heparinoid complex, in particular the solution purified from salts and undesired proteins as present to lyophilizing, is treated with immobilized protamine so as to cleave said antithrombin III-heparin complex or said antithrombin III-heparinoid complex and bind said heparin or heparinoid to said immobilized protamine, a supernatant containing said antithrombin III forming, and wherein said supernatant containing said antithrombin III is lyophilized so as to bring it into stable form.
Claim 9
Dependent Claim
108 words
A method of producing an antithrombin III concentrate from said antithrombin III-heparin concentrate or from said antithrombin III-heparinoid concentrate produced as set forth in claim 2, wherein a purified solution enriched with said antithrombin III-heparin complex or said antithrombin III-heparinoid complex, in particular the solution purified from salts and undesired proteins as present to lyophilizing, is treated with immobilized protamine so as to cleave said antithrombin III-heparin complex or said antithrombin III-heparinoid complex and bind said heparin or heparinoid to said immobilized protamine, a supernatant containing said antithrombin III forming, and wherein said supernatant containing said antithrombin III is lyophilized so as to bring it into stable form.
Claim 10
Dependent Claim
108 words
A method of producing an antithrombin III concentrate from said antithrombin III-heparin concentrate or from said antithrombin III-heparinoid concentrate produced as set forth in claim 3, wherein a purified solution enriched with said antithrombin III-heparin complex or said antithrombin III-heparinoid complex, in particular the solution purified from salts and undesired proteins as present to lyophilizing, is treated with immobilized protamine so as to cleave said antithrombin III-heparin complex or said antithrombin III-heparinoid complex and bind said heparin or heparinoid to said immobilized protamine, a supernatant containing said antithrombin III forming, and wherein said supernatant containing said antithrombin III is lyophilized so as to bring it into stable form.
Claim 11
Dependent Claim
108 words
A method of producing an antithrombin III concentrate from said antithrombin III-heparin concentrate or from said antithrombin III-heparinoid concentrate produced as set forth in claim 4, wherein a purified solution enriched with said antithrombin III-heparin complex or said antithrombin III-heparinoid complex, in particular the solution purified from salts and undesired proteins as present to lyophilizing, is treated with immobilized protamine so as to cleave said antithrombin III-heparin complex or said antithrombin III-heparinoid complex and bind said heparin or heparinoid to said immobilized protamine, a supernatant containing said antithrombin III forming, and wherein said supernatant containing said antithrombin III is lyophilized so as to bring it into stable form.
Claim 12
Dependent Claim
108 words
A method of producing an antithrombin III concentrate from said antithrombin III-heparin concentrate or from said antithrombin III-heparinoid concentrate produced as set forth in claim 5, wherein a purified solution enriched with said antithrombin III-heparin complex or said antithrombin III-heparinoid complex, in particular the solution purified from salts and undesired proteins as present to lyophilizing, is treated with immobilized protamine so as to cleave said antithrombin III-heparin complex or said antithrombin III-heparinoid complex and bind said heparin or heparinoid to said immobilized protamine, a supernatant containing said antithrombin III forming, and wherein said supernatant containing said antithrombin III is lyophilized so as to bring it into stable form.
Claim 13
Dependent Claim
108 words
A method of producing an antithrombin III concentrate from said antithrombin III-heparin concentrate or from said antithrombin III-heparinoid concentrate produced as set forth in claim 6, wherein a purified solution enriched with said antithrombin III-heparin complex or said antithrombin III-heparinoid complex, in particular the solution purified from salts and undesired proteins as present to lyophilizing, is treated with immobilized protamine so as to cleave said antithrombin III-heparin complex or said antithrombin III-heparinoid complex and bind said heparin or heparinoid to said immobilized protamine, a supernatant containing said antithrombin III forming, and wherein said supernatant containing said antithrombin III is lyophilized so as to bring it into stable form.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-04-08 | 1988-10-08 | 1989-04-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-04-08 | 1992-10-08 | 1993-04-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-04-08 | 1996-10-08 | 1997-04-09 | $7700.00 | Window Closed / Lapsed |