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US4500451A
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New protein PP13 extracted from human placental tissues and use thereof
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1983-08-18
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Granted: 1985-02-19
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Expires: 2003-08-18
Abstract
Abstract Protein PP13 is isolated from human placental tissues using a variety of methods, e.g., immunoadsorption. The protein has utility in production of an antiserum which has diagnostic applications. The placental specific proteins (e.g., PP13) are frequently detected in increasing amounts in women as pregnancy advances, and in patients with tumors especially of the tryphoblastic and embryonal types.
Inventors
- 👤 Hans Bohn
- 👤 Walter Kraus
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-04 14:29
CPC Classifications
C; C07; C07K; C07K14/00; C07K14/435; C07K14/46; C07K14/47; C07K14/4701; C07K14/4715; C07K16/00; C07K16/18; G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/68; G01N33/689; Y; Y10; Y10S; Y10S530/00; Y10S530/827; Y10S530/85; Y10S530/851
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
97 words
A new placental protein PP 13 extracted from human placental tissue characterized by the following: (a) an electrophoretic mobility in the same range as albumin, (b) an isoelectric point of 4.75±0.15, (c) a sedimentation coefficient S 20 0 .sub.,w of 3.1±0.15, (d) a molecular weight determined by ultracentrifugation of 30,000±5,000, (e) a molecular weight determined in a polyacrylamide gel containing sodium dodecyl sulfate (SDS) of 29,000±3,000, (f) an extinction coefficient E 1cm 1% (280 nm) of 9.8±0.3 and (g) a carbohydrate content of 1% (0.1±0.05% mannose, 0.2±0.1% galactose, 0.1±0.05% xylose, 0.2±0.1% glucosamine, and neuraminic acid not detected).
Claim 2
Dependent Claim
97 words
A new placental protein PP 13 extracted from human placental tissue characterized by the following: (a) an electrophoretic mobility in the same range as albumin, (b) an isoelectric point of 4.75±0.15, (c) a sedimentation coefficient S 20 0 .sub.,w of 3.1±0.15, (d) a molecular weight determined by ultracentrifugation of 30,000±5,000, (e) a molecular weight determined in a polyacrylamide gel containing sodium dodecyl sulfate (SDS) of 29,000±3,000, (f) an extinction coefficient E 1cm 1% (280 nm) of 9.8±0.3 and (g) a carbohydrate content of 1% (0.1±0.05% mannose, 0.2±0.1% galactose, 0.1±0.05% xylose, 0.2±0.1% glucosamine, and neuraminic acid not detected).
Claim 3
Dependent Claim
23 words
An antiserum specific to PP 13 obtained by immunizing an animal with protein PP 13 and recovering serum containing antibodies to said protein.
Claim 4
Dependent Claim
23 words
An antiserum specific to PP 13 obtained by immunizing an animal with protein PP 13 and recovering serum containing antibodies to said protein.
Claim 5
Dependent Claim
40 words
A process for purifying protein PP 13 comprising: (1) binding an antiserum specific to protein PP 13 to a substrate; (2) introducing a sample containing PP 13 onto said substrate; and (3) eluting the protein PP 13 from said substrate.
Claim 6
Dependent Claim
40 words
A process for purifying protein PP 13 comprising: (1) binding an antiserum specific to protein PP 13 to a substrate; (2) introducing a sample containing PP 13 onto said substrate; and (3) eluting the protein PP 13 from said substrate.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1988-02-19 | 1988-08-20 | 1989-02-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1992-02-19 | 1992-08-20 | 1993-02-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1996-02-19 | 1996-08-20 | 1997-02-19 | $7700.00 | Window Closed / Lapsed |